{"id":23056,"date":"2020-01-14T14:30:07","date_gmt":"2020-01-14T06:30:07","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=23056"},"modified":"2022-07-19T17:39:54","modified_gmt":"2022-07-19T09:39:54","slug":"cnr1-orf-vector-human-porf-%e8%b2%a8%e8%99%9forf017546","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=23056","title":{"rendered":"CNR1 ORF Vector (Human) (pORF) | \u8ca8\u865fORF017546"},"content":{"rendered":"

\u7522\u54c1\u540d\u7a31\u00a0\u00a0<\/span><\/strong>CNR1 ORF Vector (Human) (pORF) | <\/strong>\u8ca8\u865f<\/strong>ORF017546<\/span> (1.0 \u00b5g DNA)<\/p>\n

Abmgood\u63d0\u4f9b\u4e00\u7cfb\u5217\u5e8f\u5217\u9a57\u8b49\u958b\u653e\u95b1\u8b80\u6846\uff08ORF\uff09\u5eab\uff0c\u5305\u62ec\u4eba(human)\uff0c\u5c0f\u9f20(mouse)\u548c\u5927\u9f20(rat)\uff0c\u53efinsertion\u81f3\u60a8\u9078\u64c7\u7684\u86cb\u767d\u8cea\u8868\u9054\u7cfb\u7d71(protein expression systems.)\u3002\u53bb\u96645’UTR\u548c3’UTR\uff0cORF\u63d2\u5165\u517c\u5bb9\u7684\u8868\u9054\u8f09\u9ad4(compatible expression vector) \u5f8c\u5373\u53ef\u5feb\u901f\u53ef\u9760\u5730\u8868\u9054\u86cb\u767d\u8cea\u3002\u4e5f\u53ef\u7528\u4f5cPCR\u64f4\u589e (PCR amplification experiments) \u5be6\u9a57\u4e2d\u967d\u6027\u5c0d\u7167(positive control)\u7684\u6a21\u677f(template)\u3002Abmgood\u63d0\u4f9b\u7684ORF\u5305\u542b\u4e00\u500b\u7a81\u8b8a\u7684\u7d42\u6b62\u5bc6\u78bc\u5b50(mutated stop codon)\uff0c\u5982\u679c\u53d7\u9ad4\u8f09\u9ad4\u4e2d\u5b58\u5728C\u672b\u7aef\u6a19\u7c64\u6216\u5831\u544a\u57fa\u56e0\uff0c\u5247\u5141\u8a31\u5176\u647b\u5165\u3002 abm\u7684ORF\u4e5f\u53ef\u4ee5\u4ee5\u5373\u7528\u578b\u86cb\u767d\u8868\u9054\u8f09\u9ad4(protein expression vectors<\/a>)\uff0c\u6162\u75c5\u6bd2\u8f09\u9ad4(lentiviral vectors<\/a>)\u6216\u5305\u88dd\u7684\u6162\u75c5\u6bd2(\u00a0lentivirus<\/a>)\u548c\u817a\u75c5\u6bd2(\u00a0adenovirus\u00a0<\/a>)\u4e4b\u67b6\u69cb\u597d\u4e4b\u8f09\u9ad4\u6216\u75c5\u6bd2\u6db2\u5f62\u5f0f\u63d0\u4f9b\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39: CNR1 ORF\u8f09\u9ad4\uff0c\u57fa\u56e0Accession Number: NM_001160226.1<\/a>\uff0cCNR1\u57fa\u56e0\u9577\u5ea6\u70ba1419bp (Human)\uff0c\u67b6\u69cb\u65bcpORF<\/a>\u8f09\u9ad4\u3002\u5728AflII\u548cEcoRV\u5207\u5272\u4f4d\u9ede\u4e4b\u9593\u4fdd\u7559\u4e86\u8a72\u57fa\u56e0\uff08\u9650\u5236\u6027\u9176\u5207\u65b9\u6cd5\uff09\u3002Inserts\u4e0d\u5305\u542b\u4efb\u4f55\u5167\u90e8AflII\u548cEcoRV cut site\uff0c\u53ef\u4ee5AflII\u548cEcoRV\u5207\u9664\u3002\u5728\u63d2\u5165\u7247\u6bb5\u5305\u542b\u9019\u4e9b\u5167\u90e8\u5207\u5272\u4f4d\u9ede\u7684\u60c5\u6cc1\u4e0b\uff0c\u53ef\u4ee5PCR\u64f4\u589e\u5e36\u6709\u512a\u9078\u9650\u5236\u6027\u9176\u5207\u4f4d\u9ede\u7684\u63d2\u5165\u7247\u6bb5\uff0c\u4ee5\u9032\u884csubcloning\u3002\u7be9\u9078marker: (\u7d30\u83ccSpectinomycin)\u3002<\/p>\n\n\n\n\n\n
ector Name<\/td>\npORF<\/td>\n<\/tr>\n
Antibiotic Information<\/td>\nBacterial: Spectinomycin<\/td>\n<\/tr>\n
Sequencing Primers<\/td>\nM13 FP: 5′-CCCAGTCACGACGTTGTAAAACG-3′
\nM13 RP: 5′-CAGGAAACAGCTATGAC-3′<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Insert<\/strong><\/td>\nCNR1<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nThe CNR1 ORF Vector holds the gene (cloned by a restriction enzyme-independent method) between an AflII and EcoRV cut site. Inserts are flanked by and can be excised using AflII and EcoRV as long as inserts do not contain any internal AflII and EcoRV sites. In the case that the insert contains these internal cut sites, the end user can PCR amplify the insert with preferred restriction sites for subcloning.<\/td>\n<\/tr>\n
Accession Number<\/strong><\/td>\nNM_001160226.1<\/a><\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npORF<\/a><\/td>\n<\/tr>\n
Vector Size<\/strong><\/td>\n2596bp<\/td>\n<\/tr>\n
Insert Size<\/strong><\/td>\n1419bp<\/td>\n<\/tr>\n
Species<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Selection Marker<\/strong><\/td>\nBacterial: Spectinomycin<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nClear Liquid<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Stability<\/strong><\/td>\n1 year when stored at -20\u00b0C or lower in a non-frost free freezer.<\/td>\n<\/tr>\n
Caution<\/strong><\/td>\nCNR1 ORF Vector (Human) (pORF) is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/p>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u7522\u54c1\u540d\u7a31\u00a0\u00a0CNR1 ORF Vector (Human) (pORF) | \u8ca8\u865fORF017546 (1.0 […]<\/p>\n","protected":false},"author":3,"featured_media":14324,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[88],"tags":[1732],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23056"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=23056"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23056\/revisions"}],"predecessor-version":[{"id":23057,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23056\/revisions\/23057"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/14324"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=23056"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=23056"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=23056"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}