{"id":22922,"date":"2020-01-10T15:42:11","date_gmt":"2020-01-10T07:42:11","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22922"},"modified":"2022-07-19T17:42:24","modified_gmt":"2022-07-19T09:42:24","slug":"luciferase-firefly-lentiviral-vector-cmv-%e6%85%a2%e7%97%85%e6%af%92%e8%bc%89%e9%ab%94-%e8%b2%a8%e8%99%9flv010162","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=22922","title":{"rendered":"LUCIFERASE (FIREFLY) Lentiviral Vector (CMV) \u6162\u75c5\u6bd2\u8f09\u9ad4 | \u8ca8\u865fLV010162"},"content":{"rendered":"

LUCIFERASE (FIREFLY) Lentiviral Vector (CMV)<\/strong> \u6162\u75c5\u6bd2\u8f09\u9ad4<\/strong> | <\/strong>\u8ca8\u865f<\/strong>LV010162<\/strong><\/a><\/span><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>: <\/strong>LUCIFERASE (FIREFLY) Lentiviral Vector (CMV)<\/u>\u6162\u75c5\u6bd2\u8868\u73fe\u8f09\u9ad4\u3002mKate2\u901a\u904e2A\u80bd\u8207\u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176\u5831\u544a\u57fa\u56e0\u9023\u63a5\uff0c\u8868\u9054\u7531CMV\u555f\u52d5\u5b50\u9a45\u52d5\u3002\u57fa\u56e0Luciferase (Firefly)\u67b6\u69cb\u65bcpLenti-III-CMV<\/a>\u8868\u73fe\u8f09\u9ad4\uff0c\u7531CMV\u555f\u52d5\u5b50\u9a45\u52d5\uff0c\u6c92\u6709\u6a19\u7c64Tag\u3002\u53ef\u76f4\u63a5\u4ee5\u975e\u75c5\u6bd2\u8cea\u7c92\u9032\u884c\u8f49\u67d3\u7528\u65bc\u5373\u6642\u8868\u9054\uff0c\u6216\u5305\u88dd\u6210\u6162\u75c5\u6bd2\u9846\u7c92\u7528\u65bc\u9ad8\u6548\u8f49\u5c0e\u548c\u7a69\u5b9a\u6574\u5408\u8868\u9054\u3002\u75c5\u6bd2\u5305\u88dd\u7cfb\u7d71\u5efa\u8b70\u4f7f\u7528ABMGOOD<\/strong>\u63d0\u4f9b\u4e4b\u8f49\u67d3\u8a66\u5291<\/strong> Cat#\u00a0G074, Lentifectin\u2122<\/a> <\/span>\u53ca\u512a\u5316\u4e4b\u75c5\u6bd2\u5305\u88dd\u7cfb\u5217\u7522\u54c1LV003<\/a><\/span>\u3002\u6162\u75c5\u6bd2\u5305\u88dd\u7cfb\u5217\u7522\u54c1\u53e6\u5305\u62ecLentivirus<\/u>\u6162\u75c5\u6bd2\u4f4e\u901f\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>\/\u00a0Lentivirus <\/u>\u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>\u4e26\u4ee5\u00a0Lentivirus<\/u>\u75c5\u6bd2<\/u>RT-QPCR<\/u>\u5b9a\u91cf<\/u>(\u5b9a\u91cf)<\/a>\u00a0\u7d14\u5316\u5f8c\u4e4b\u75c5\u6bd2\u529b\u50f9\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/td>\nPrint Version<\/a><\/td>\n<\/tr>\n
Description<\/strong><\/td>\nmKate2 linked to a firefly Luciferase reporter by a 2A peptide, expression driven by CMV promoter.<\/td>\n<\/tr>\n
Product Application<\/strong><\/td>\n\n

\u00b7\u00a0 Direct non-viral plasmid transfection for immediate expression<\/p>\n

\u00b7\u00a0 Package into Lentiviral particles for high efficiency transduction and stably integrated expression<\/td>\n<\/tr>\n

Gene Insert<\/strong><\/td>\nLuciferase (Firefly)<\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npLenti-III-CMV<\/a><\/td>\n<\/tr>\n
Vector Size<\/strong><\/td>\n10506 bp<\/td>\n<\/tr>\n
Insert Size<\/strong><\/td>\n0 bp<\/td>\n<\/tr>\n
Reporter\/Tag<\/strong><\/td>\nNo Tags<\/td>\n<\/tr>\n
Promoter<\/strong><\/td>\nCMV<\/td>\n<\/tr>\n
Selection Marker<\/strong><\/td>\nBacterial: Kanamycin; Mammalian: Puromycin<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nPlasmid<\/td>\n<\/tr>\n
Viral Packaging<\/strong><\/td>\nabm<\/strong>\u00a0recommends using Cat#\u00a0G074, Lentifectin\u2122<\/a>\u00a0in conjunction with one of the following packaging mixes. The suggested packaging mix for this vector is Cat#\u00a0LV003, 2nd Generation Packaging Mix<\/a>. Alternatively, you can also use Cat#\u00a0LV053, 3rd Generation Packaging Mix<\/a><\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nClear Liquid<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Stability<\/strong><\/td>\n1 year when stored at -20\u00b0C or lower in a non-frost free freezer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

LUCIFERASE (FIREFLY) Lentiviral Vector (CMV) \u6162\u75c5\u6bd2\u8f09\u9ad4 | \u8ca8\u865f […]<\/p>\n","protected":false},"author":3,"featured_media":22923,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1,3],"tags":[1741],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22922"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22922"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22922\/revisions"}],"predecessor-version":[{"id":22926,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22922\/revisions\/22926"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/22923"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22922"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22922"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22922"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}