{"id":22878,"date":"2020-01-07T12:03:19","date_gmt":"2020-01-07T04:03:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22878"},"modified":"2022-07-19T17:44:01","modified_gmt":"2022-07-19T09:44:01","slug":"minute%e6%a4%8d%e7%89%a9%e5%be%ae%e7%b2%92%e9%ab%94%e8%86%9c%e6%8f%90%e5%8f%96%e8%a9%a6%e5%8a%91-invent%e8%b2%a8%e8%99%9fmm-018","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=22878","title":{"rendered":"Minute\u2122\u690d\u7269\u5fae\u7c92\u9ad4\u819c\u63d0\u53d6\u8a66\u5291| Invent\u8ca8\u865fMM-018"},"content":{"rendered":"

\u7522\u54c1\u540d\u7a31<\/strong>:<\/strong> Minute\u2122\u690d\u7269\u5fae\u7c92\u9ad4\u819c\u63d0\u53d6\u8a66\u5291\u76d2\uff0850\u500b\u5206\u6790\uff09| Invent<\/strong>\u8ca8\u865f<\/strong>MM-018<\/a><\/span><\/p>\n

Minute\u2122 Plant Microsomal Membrane Extraction Kit (50 Preps)<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u5f9e\u690d\u7269\u7d44\u7e54(plant tissues)\u4e2d\u5206\u96e2\u5fae\u7c92\u9ad4\u819c(microsomal membranes)\u662f\u4e00\u7a2e\u5e38\u898b\u65b9\u6cd5\u3002\u690d\u7269\u7d30\u80de\u88c2\u89e3\u7269\u7684\u5fae\u7c92\u9ad4\u90e8\u5206(Microsomal fraction)\u662f\u8a31\u591a\u690d\u7269\u7814\u7a76\u9805\u76ee\u4e2d\u95dc\u6ce8\u7684\u7126\u9ede\uff0c\u4e26\u4e14\u64da\u4fe1\u5bcc\u542b\u7d30\u80de\u8cea\u819c(plasma membranes)\uff0c\u5167\u8cea\u7db2(endoplasmic reticulum)\uff0c\u9ad8\u723e\u57fa\u9ad4(Golgi apparatus)\uff0c\u6db2\u6ce1\u819c(vacuolar membranes)\u548c\u819c\u7cfb\u7d71(membrane system)\u7684\u5176\u4ed6\u7d44\u4ef6\u3002\u7528\u65bc\u5fae\u7c92\u9ad4\u7d1a\u5206\u5206\u96e2\u7684\u50b3\u7d71\u65b9\u6cd5(Traditional methods)\u5305\u62ec\u5dee\u5206\u6c89\u6fb1\u6cd5(differential pelleting)\uff0c\u5176\u4e2d\u819c\u7d1a\u5206\u9700\u8981\u96e2\u5fc3\u6b65\u9a5f\u3002\u5fae\u7c92\u9ad4\u7d1a\u5206\u7684\u5206\u96e2(microsomal fraction isolation)\u901a\u5e38\u9700\u8981\u5927\u91cf\u7684\u8d77\u59cb\u539f\u6599\uff0c\u4e26\u4e14\u5177\u6709\u7e41\u7463\u7684\u8d85\u901f\u96e2\u5fc3\u6b65\u9a5f\u3002 MM-018\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\uff0c\u5feb\u901f\u3001user-friendly\u7684\u65b9\u6cd5\uff0c\u53ef\u4f7f\u7528\u5c11\u91cf\u8d77\u59cb\u539f\u6599\u4f86\u63d0\u53d6\u5fae\u7c92\u9ad4\u819c (microsomal membrane extraction)\u3002\u5728\u64cd\u4f5c\u904e\u7a0b\u4e2d\uff0c\u5c07\u6c34\u6eb6\u6027\u80de\u8cea\u86cb\u767d(water-soluble cytosolic proteins)\u53bb\u9664\uff0c\u4e26\u5728\u4f4e\u901f\u5fae\u91cf\u96e2\u5fc3\u6a5f\u4e2d\u7528\u512a\u5316\u7684\u7de9\u885d\u6db2\u63d0\u53d6\u6c34\u4e0d\u6eb6\u6027\u5fae\u7c92\u9ad4\u90e8\u4efd\uff0c\u5c24\u5176\u662f\u8cea\u819c\u90e8\u4efd(plasma membrane fraction)\u3002Minute\u2122\u690d\u7269\u5fae\u7c92\u9ad4\u819c\u63d0\u53d6\u8a66\u5291\u76d2 (\u8ca8\u865f<\/strong>MM-018)\uff0c\u7121\u9700\u8d85\u901f\u96e2\u5fc3(without ultracentrifugation)\u5373\u53ef\u5728\u7d04\u4e00\u5c0f\u6642\u5167\u5f9e\u690d\u7269\u7d44\u7e54\u4e2d\u5206\u96e2\u51fa\u5929\u7136\u5fae\u7c92\u9ad4\u86cb\u767d\u3002\u86cb\u767d\u8cea\u7522\u91cf\u70ba100-200 \u00b5g \/\u6a23\u54c1\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n
\u00a0Minute\u2122\u690d\u7269\u5fae\u7c92\u9ad4\u819c\u63d0\u53d6\u8a66\u5291\u76d2<\/td>\nQuantity<\/strong><\/td>\n<\/tr>\n
Buffer A<\/td>\n25 ml<\/td>\n<\/tr>\n
Buffer B<\/td>\n10 ml<\/td>\n<\/tr>\n
Filter Cartridges<\/td>\n50 units<\/td>\n<\/tr>\n
2.0 ml Collection Tubes with Caps<\/td>\n50 units<\/td>\n<\/tr>\n
Plastic Rods<\/td>\n2 units<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Minute\u2122<\/strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300e<\/strong>Column Filter<\/strong>\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong>\u00a0<\/strong>\u4e00<\/strong> \u5206\u9418\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<\/strong>vortex<\/em><\/strong>\u3001<\/em><\/strong>transfer<\/em><\/strong>\u3001<\/em><\/strong>spin <\/em><\/strong>\u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n

\u6587\u737b\u5f15\u7528<\/p>\n

    \n
  1. Kim, S., Choi, Y., Kwon, C., & Yun, H. S. (2018). Endoplasmic reticulum stress\u2010induced accumulation of VAMP721\/722 requires CALRETICULIN 1 and CALRETICULIN 2 in Arabidopsis.\u00a0Journal of integrative plant biology<\/em>.<\/li>\n<\/ol>\n

    <\/strong>\u86cb\u767d\u8cea\u8403\u53d6\u71b1\u9580\u5546\u54c1 \u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44<\/a> | \u8cea\u819c\u86cb\u767d\u8403\u53d6\u5957\u7d44<\/a> |<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    Product Name<\/td>\nCatalog No.<\/td>\n<\/tr>\n
    Minute TM<\/sup> Total Protein Extraction Kit\u00a0 for Animal Cultured Cells\/Tissues (50 Tests)<\/td>\nSD-001\/SN-002<\/td>\n<\/tr>\n
    Minute TM<\/sup> Cytoplasmic and Nuclear Fractionation kit (50 Tests)<\/td>\nSC-003<\/td>\n<\/tr>\n
    Minute TM<\/sup> Detergent-Free Total Protein Extraction Kit for Animal Cells\/Tissues (50 Tests)<\/td>\nSN-006<\/td>\n<\/tr>\n
    Minute TM<\/sup> Plasma Membrane Protein Isolation Kit (50 Tests)<\/td>\nSM-005<\/td>\n<\/tr>\n
    Minute TM<\/sup> Plasma Membrane Protein Isolation Kit for Plants (50 Tests)<\/td>\nSM-005-p<\/td>\n<\/tr>\n
    Minute TM <\/sup>Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests)<\/td>\nMP-007<\/td>\n<\/tr>\n
    Minute TM <\/sup>Mitochondria Isolation Kit for Muscle Tissues\/Cultured Muscle Cells (50 Tests) NEW!<\/td>\nMM-038<\/td>\n<\/tr>\n
    Minute TM <\/sup>Total Protein Extraction Kit for Plant Tissues (50 Tests)<\/td>\nSD-008\/SN-009<\/td>\n<\/tr>\n
    Minute TM <\/sup>Detergent-Free Plant Protein Extraction Kit (50 Tests)<\/td>\nSN-010<\/td>\n<\/tr>\n
    Minute TM<\/sup> Chloroplast Isolation Kit (50 Tests)<\/td>\nCP-011<\/td>\n<\/tr>\n
    Minute TM <\/sup>Bacterial Total Protein Extraction Kit (50 Tests)<\/td>\nSB-004<\/td>\n<\/tr>\n
    Minute TM<\/sup> Single Cell Isolation Kit (50 Tests)<\/td>\nSC-012<\/td>\n<\/tr>\n
    Minute TM<\/sup> Nuclear Envelope Protein Extraction Kit (50 Tests)<\/td>\nNE-013<\/td>\n<\/tr>\n
    Minute TM<\/sup> Histon\/DNA Binding\u00a0 Protein Extraction Kit (50 Tests)<\/td>\nHP-014<\/td>\n<\/tr>\n
    Minute\u2122 Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\nYT-015<\/td>\n<\/tr>\n
    Minute\u2122 Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\nYD-016<\/td>\n<\/tr>\n
    Minute\u2122 Yeast Mitochondria Enrichment Kit (50 tests)<\/td>\nYM-017<\/td>\n<\/tr>\n
    Minute\u2122 Plant Microsomal Membrane Extraction Kit (50 Tests)<\/td>\nMM-018<\/td>\n<\/tr>\n
    Minute\u2122 Protein\/Nucleic Acid Extraction Kit from Gels (20 tests)<\/td>\nPN-019<\/td>\n<\/tr>\n
    MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests)<\/td>\nHD-021<\/td>\n<\/tr>\n
    MinuteTM Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Tests)<\/td>\nAT-022<\/td>\n<\/tr>\n
    MinuteTM Adipose Tissue Fractionation Kit (20 Tests)<\/td>\nAF-023<\/td>\n<\/tr>\n
    MinuteTM <\/sup>Detergent-Free Nuclei Isolation Kit (20 Tests)<\/td>\nNI-024<\/td>\n<\/tr>\n
    MinuteTM <\/sup>Single Nucleus Isolation Kit for Neuronal Tissues\/Cells (20 preps) NEW!<\/td>\nBN-020<\/td>\n<\/tr>\n
    MinuteTM <\/sup>Single Nucleus Isolation Kit for Tissues\/Cells (20 preps) NEW!<\/td>\nSN-047<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Nuclei and Cytosol Isolation Kit for Adipose Tissues (20 Tests)<\/td>\nAN-029<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests)<\/td>\nFE-025<\/td>\n<\/tr>\n
    MinuteTM <\/sup>Total Protein Extraction Kit for Mass Spectrometry (50 Tests)<\/td>\nMS-026<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG)<\/td>\nEI-027<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Endosome Isolation and Cell Fractionation Kit (20 Tests)<\/td>\nED-028<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG)<\/td>\nSE-030<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Cell Suspension Isolation from Fresh\/Frozen Tissues (50 tests)<\/td>\nCS-031<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tissues (20 tests)<\/td>\nNT-032<\/td>\n<\/tr>\n
    MinuteTM <\/sup>Detergent-Free Nuclear Matrix Protein Extraction Kit (20 tests)<\/td>\nNM-033<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Lysosome Isolation Kit (20 tests)<\/td>\nLY-034<\/td>\n<\/tr>\n
    MinuteTM<\/sup> High Fidelity ER Isolation Kit for Liver Tissue (20 tests)<\/td>\nER-035<\/td>\n<\/tr>\n
    MinuteTM<\/sup> ER Enrichment Kit\u00a0 (20 tests)<\/td>\nER-036<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Golgi Apparatus Enrichment Kit (20 tests)<\/td>\nGO-037<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests) NEW!<\/td>\nLR-039<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests) NEW!<\/td>\nLR-042<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Cytosolic Proteasome Enrichment Kit (20 tests) NEW!<\/td>\nPT-040<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Nuclear Proteasome Enrichment Kit (20 tests) NEW!<\/td>\nPN-041<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests) NEW!<\/td>\nPF-045<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Native Protein Precipitation Kit from Saliva (20 tests) NEW!<\/td>\nSV-043<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Organic Solvent-Free Milk Lipid Depletion Kit (20 tests) NEW!<\/td>\nML-044<\/td>\n<\/tr>\n
    MinuteTM<\/sup> Rubisco Depletion Kit (20 tests) NEW!<\/td>\nRD-046<\/td>\n<\/tr>\n
    MinuteTM <\/sup>Total Protein Extraction Kit for Skin Tissue (50 tests)<\/td>\nSA-01-SK<\/td>\n<\/tr>\n
    MinuteTM <\/sup>Total Protein Extraction Kit for Bone Tissue (50 tests)<\/td>\nSA-02-BT<\/td>\n<\/tr>\n
    MinuteTM <\/sup>Total Protein Extraction Kit for Blood Vessels (50 tests)<\/td>\nSA-03-BV<\/td>\n<\/tr>\n
    MinuteTM <\/sup>Total Protein Extraction Kit for Tendons (50 tests)<\/td>\nSA-04-TD<\/td>\n<\/tr>\n
    MinuteTM <\/sup>Total Protein Extraction Kit for Intestines and Mesentery (50 tests)<\/td>\nSA-05-IM<\/td>\n<\/tr>\n
    MinuteTM <\/sup>Total Protein Extraction Kit for Muscles (50 tests)<\/td>\nSA-06-MS<\/td>\n<\/tr>\n
    MinuteTM <\/sup>Total Protein Extraction Kit for Insects (50 tests)<\/td>\nSA-07-IS<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u7522\u54c1\u540d\u7a31: Minute\u2122\u690d\u7269\u5fae\u7c92\u9ad4\u819c\u63d0\u53d6\u8a66\u5291\u76d2\uff0850\u500b\u5206\u6790\uff09| Invent\u8ca8\u865fMM-018 Minute\u2122 […]<\/p>\n","protected":false},"author":3,"featured_media":4646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[97],"tags":[1753],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22878"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22878"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22878\/revisions"}],"predecessor-version":[{"id":22879,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22878\/revisions\/22879"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4646"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22878"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22878"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22878"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}