{"id":22671,"date":"2019-12-26T15:43:53","date_gmt":"2019-12-26T07:43:53","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22671"},"modified":"2022-07-19T17:46:33","modified_gmt":"2022-07-19T09:46:33","slug":"dhfr%e4%ba%8c%e6%b0%ab%e8%91%89%e9%85%b8%e9%82%84%e5%8e%9f%e9%85%b6%e6%b4%bb%e6%80%a7%e8%a9%a6%e5%8a%91-dihydrofolate-reductase-activity-kit%e8%b2%a8%e8%99%9fk246-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=22671","title":{"rendered":"DHFR\u4e8c\u6c2b\u8449\u9178\u9084\u539f\u9176\u6d3b\u6027\u8a66\u5291 | Dihydrofolate Reductase Activity Kit\u8ca8\u865fK246-100"},"content":{"rendered":"

DHFR\u4e8c\u6c2b\u8449\u9178\u9084\u539f\u9176\u6d3b\u6027\u8a66\u5291 | <\/strong>\u8ca8\u865f<\/strong>K246-100<\/a><\/span><\/p>\n

Dihydrofolate Reductase Activity Kit (Colorimetric)<\/p>\n

\u00a0<\/em><\/strong>\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u4e8c\u6c2b\u8449\u9178\u9084\u539f\u9176\uff08Dihydrofolate Reductase , DHFR\uff1b 5,6,7,8-\u56db\u6c2b\u8449\u9178NADP\u6c27\u5316\u9084\u539f\u9176\uff1b EC 1.5.1.3\uff09\uff0c\u662f\u4e00\u7a2e\u666e\u904d\u5b58\u5728\u7684\u9176\uff0c\u5b58\u5728\u65bc\u6240\u6709\u771f\u6838(eukaryotic cells)\u548c\u539f\u6838\u7d30\u80de(prokaryotic cells)\u4e2d\u3002\u5b83\u4f7f\u7528NADPH\u4f5c\u70ba\u8f14\u52a9\u56e0\u5b50\u50ac\u5316\u4e8c\u6c2b\u8449\u9178\uff08dihydrofolate , FH2\uff09\u9084\u539f\u70ba\u56db\u6c2b\u8449\u9178\uff08tetrahydrofolate , FH4\uff09\u3002 FH4\u5c0d\u65bc\u560c\u5464(purines)\uff0c\u80f8\u82f7\u9178(thymidylic acid)\u548c\u67d0\u4e9b\u6c28\u57fa\u9178\u5f9e\u982d\u5408\u6210\u6240\u9700\u7684\u591a\u7a2e\u9176\u81f3\u95dc\u91cd\u8981\u3002 DHFR\u9176\u6d3b\u6027\u7684\u5931\u6d3b\u5c0e\u81f4\u7d30\u80de\u5167FH4\u6c34\u5e73\u964d\u4f4e\uff0cRNA\u548cDNA\u5408\u6210\u6291\u5236\u4ee5\u53ca\u7d30\u80de\u6b7b\u4ea1\u3002\u56e0\u6b64\uff0cDHFR\u4f5c\u70ba\u85e5\u7269\u958b\u767c\u4e2d\u7684\u5206\u5b50\u9776\u6a19\u5df2\u6210\u70ba\u81f3\u95dc\u91cd\u8981\u7684\u9176\u3002 Biovision\u7684\u4e8c\u6c2b\u8449\u9178\u9084\u539f\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2(Biovision\u2019s Dihydrofolate Reductase assay kit) \u57fa\u65bcDHFR\u50ac\u5316NADPH\u6c27\u5316\u7684\u80fd\u529b\u3002\u901a\u904e\u76e3\u6e2c340nm\u8655\u5438\u5149\u5ea6\u7684\u4e0b\u964d\u4f86\u8ddf\u8e2a\u53cd\u61c9\u9032\u7a0b\u3002\u6211\u5011\u7684\u6e2c\u5b9a\u6cd5\u5df2\u7d93\u904e\u512a\u5316\uff0c\u53ef\u4ee5\u572896\u5b54\u677f\u4e2d\u9032\u884c\u3002\u8a72\u6e2c\u5b9a\u65b9\u6cd5\u7c21\u55ae\uff0c\u9748\u654f\uff0c\u4e26\u4e14\u5728\u5404\u7a2e\u6a23\u54c1\u4e2d\u5747\u53ef\u6aa2\u6e2c\u5230\u4f4e\u81f34 mU \/ ml\u7684\u6fc3\u5ea6\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK246-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (OD=340 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetection of Dihydrofolate Reductase activity<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Rapid, sensitive and convenient
\n\u2022The kit measures total Activity of DHFR Activity with limit of quantification 4 mU\/ml using recombinant DHFR<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 DHFR Assay Buffer
\n\u2022 DHFR Substrate
\n\u2022 Dihydrofolate Reductase
\n\u2022 NADPH<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-70\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nDry Ice<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0Finnpipette F3 \u5fae\u91cf\u5206\u6ce8\u5668<\/a>\u00a0|\u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668<\/strong> Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0Genereach\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/a>\u00a0 |\u00a0\u00a0<\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n

\u00a0<\/strong><\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

DHFR\u4e8c\u6c2b\u8449\u9178\u9084\u539f\u9176\u6d3b\u6027\u8a66\u5291 | \u8ca8\u865fK246-100 Dihydrofolate Reductase Ac […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1,3],"tags":[1775],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22671"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22671"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22671\/revisions"}],"predecessor-version":[{"id":22672,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22671\/revisions\/22672"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22671"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22671"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22671"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}