{"id":22406,"date":"2019-10-28T15:47:55","date_gmt":"2019-10-28T07:47:55","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22406"},"modified":"2025-03-21T14:33:19","modified_gmt":"2025-03-21T06:33:19","slug":"%e4%ba%ba%e5%88%9d%e4%bb%a3%e8%83%8e%e7%9b%a4%e5%be%ae%e8%a1%80%e7%ae%a1%e5%85%a7%e7%9a%ae%e7%b4%b0%e8%83%9e-human-primary-placental-microvascular-endothelial-cells-%e8%b2%a8%e8%99%9ft5449","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=22406","title":{"rendered":"\u4eba\u521d\u4ee3\u80ce\u76e4\u5fae\u8840\u7ba1\u5167\u76ae\u7d30\u80de | Human Primary Placental Microvascular Endothelial Cells \u8ca8\u865fT5449"},"content":{"rendered":"

A<\/strong>bmgood <\/strong>\u6b63\u5e38\u521d\u4ee3\u7d30\u80de\u7cfb\u5217\u5546\u54c1<\/strong> (Primary cell)<\/strong>\u00a0<\/strong><\/p>\n

\u4eba\u521d\u4ee3\u80ce\u76e4\u5fae\u8840\u7ba1\u5167\u76ae\u7d30\u80de\u00a0\u00a0|\u00a0\u8ca8\u865f\u00a0 T5449 \u00a05×105<\/sup>\u00a0cells \/ 1.0 ml<\/a><\/span><\/p>\n

Human Primary Placental Microvascular Endothelial Cells<\/p>\n

\u521d\u4ee3\u7d30\u80de (Primary Cell) \u662f\u6307\u76f4\u63a5\u5f9e\u6d3b\u9ad4\u7d44\u7e54\u4e2d\u5206\u96e2\u51fa\u7684\u7d30\u80de\uff0c\u5177\u6709\u6709\u9650\u7684\u7d30\u80de\u58fd\u547d\u7121\u6cd5\u7121\u9650\u5236\u5730\u7e7c\u4ee3\u57f9\u990a\uff1b\u8207\u4e00\u822c\u5b78\u8853\u4e0a\u5e38\u7528\u7684\u7d30\u80de\u682a (Cell Line)\u4e0d\u540c\uff0c\u5177\u6709\u6709\u9650\u7684\u7d30\u80de\u58fd\u547d\u7121\u6cd5\u7121\u9650\u5236\u5730\u7e7c\u4ee3\u57f9\u990a\uff0c\u4f46\u537b\u5177\u6709\u6700\u63a5\u8fd1\u6d3b\u9ad4\u72c0\u614b\u7684\u7d30\u80de\u578b\u614b\u8207\u529f\u80fd\u7279\u6027\u3002\u00a0<\/strong><\/p>\n

Human Primary Placental Microvascular Endothelial Cells<\/strong>\u7d30\u80deAdherent | \u4f86\u6e90Placenta (Homo sapiens) | T5449 \u3002\u7d30\u80deMarker: CD31, VE-Cadherin\u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a><\/span>)\uff0c\u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2Prigrow X series (\u8ca8\u865fTM104<\/a><\/span>)\u3002\u7d30\u80de\u5fc5\u9808\u5728\u660e\u81a0\u5305\u88ab\u7684\u5e73\u677f\u4e0a\u751f\u9577\u3002\u660e\u81a0\u5857\u6599\u6eb6\u6db2\uff080.1\uff05\uff09\u53ef\u4ee5\u5f9eAbm TM063\u7372\u5f97\u3002\u7d30\u80de\u53ea\u80fd\u4ee51\uff1a2\u7684split ratio\u6700\u591a2\u4ee3\u3002\u5efa\u8b70\u6bcf3\u5929\u9032\u884c\u4e00\u6b21medium\u66f4\u63db\u4ee5\u4fdd\u6301\u6700\u4f73\u6210\u9577\u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C.<\/p>\n

Human Primary Placental Microvascular Endothelial Cells<\/strong>\u5f9e\u6b63\u5e38\u4eba\u809d\u7d44\u7e54\u4e2d\u5206\u96e2\u51fa\u4eba\u539f\u4ee3\u6b63\u5e38\u80ce\u76e4\u5fae\u8840\u7ba1\u5167\u76ae\u7d30\u80de\u3002\u9019\u4e9b\u7d30\u80de\u986f\u793a\u51fa\u5177\u6709\u6697\u6838\u7684\u9d5d\u5375\u77f3\u72c0\u5f62\u614b\u3002\u5b83\u5011\u8868\u73fe\u51faCD31\u548c\/\u6216VE-\u9223\u9ecf\u8457\u86cb\u767d\u5167\u76ae\u7d30\u80de\u6a19\u8a8c\u7269(VE-Cadherin endothelial cell markers) \u7684\u8868\u9054\u3002\u4e00\u7a2e\u6709\u50f9\u503c\u7684\u5de5\u5177\uff0c\u53ef\u7528\u65bc\u7d30\u80de\u8207\u7d30\u80de\u7684\u7c98\u9644\u6e2c\u5b9a(cell adhesion assays)\uff0c\u6216\u7528\u65bc\u4e0b\u6e38\u904e\u7a0b\uff0c\u4f8b\u5982\u7528\u65bc\u7814\u7a76\u61c9\u7528\u7684RT-PCR\u6216Western blotting\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Organism<\/strong><\/td>\nHomo sapiens<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nPlacenta<\/td>\n<\/tr>\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nCobble-stone<\/td>\n<\/tr>\n
Isolation<\/strong><\/td>\nIsolated from normal human placental tissue<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nHuman Primary Normal Placental Microvascular Endothelial Cells are isolated from normal human liver tissue. The cells exhibit a cobblestone-like morphology with dark nuclei. They exhibit expression of CD31, and\/or VE-Cadherin endothelial cell markers. A valuable tool for cell to cell adhesion assays, or for downstream processes such as RT-PCR or Western blotting for research applications.<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Markers<\/strong><\/td>\nCD31, VE-Cadherin<\/td>\n<\/tr>\n
Propagation Requirements<\/strong><\/td>\nUse of PriCoat\u2122 T25 Flasks (G299<\/a>) or Applied Cell Extracellular Matrix (G422<\/a>) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions.<\/strong>\u00a0The base medium for this cell line is Prigrow X series medium available at\u00a0abm<\/strong>, Cat. No.\u00a0TM104<\/a>. Cells must be grown on gelatin coated plates. Gelatin Coating Solution (0.1%) is available at\u00a0abm<\/strong>, TM063. Cells can only propagate for a maximum of 2 passages at split ratio of 1:2. Recommend to conduct media change every 3rd day to maintain optimal growth.
\nCarbon dioxide (CO2<\/sub>): 5%, Temperature: 37.0\u00b0C.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

HOT!!<\/strong>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a>PriCoat\u2122 T25 Flasks | type I collagen coating | <\/a>I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix | <\/a>Prigrow III Medium\u521d\u4ee3\u7d30\u80de\u57f9\u990a\u6db2<\/a><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood \u6b63\u5e38\u521d\u4ee3\u7d30\u80de\u7cfb\u5217\u5546\u54c1 (Primary cell)\u00a0 \u4eba\u521d\u4ee3\u80ce\u76e4\u5fae\u8840\u7ba1\u5167\u76ae\u7d30\u80de\u00a0\u00a0|\u00a0\u8ca8\u865f\u00a0 […]<\/p>\n","protected":false},"author":3,"featured_media":11653,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[494],"tags":[1849],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22406"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22406"}],"version-history":[{"count":5,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22406\/revisions"}],"predecessor-version":[{"id":50808,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22406\/revisions\/50808"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/11653"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22406"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22406"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22406"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}