{"id":22375,"date":"2019-10-24T14:35:40","date_gmt":"2019-10-24T06:35:40","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22375"},"modified":"2022-07-20T09:32:56","modified_gmt":"2022-07-20T01:32:56","slug":"letheen-agar-%e7%93%8a%e8%84%82-%e8%b2%a8%e8%99%9flab185","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=22375","title":{"rendered":"Letheen Agar \u74ca\u8102 (\u5375\u78f7\u8102) | \u8ca8\u865fLAB185"},"content":{"rendered":"

6<\/strong>\u516c\u5347\u8ff7\u4f60\u57f9\u990a\u7bb1<\/strong><\/a> | \u83cc\u6db2\u6e2c\u91cf<\/strong><\/a>\u00a0<\/strong>(<\/strong>\u5206\u5149\u5149\u5ea6\u8a08<\/strong>)<\/strong>\u00a0<\/strong>\u00a0|\u00a0<\/u>\u6ec5\u83cc\u91dc<\/strong><\/a>\u00a0 |\u00a0<\/strong>\u6c34\u6d74\u69fd<\/strong>WaterBath<\/strong><\/em><\/a>\u00a0|\u00a0<\/strong>\u96fb\u5b50\u5929\u5e73<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>pH<\/strong>\u9178\u9e7c\u5ea6\u8a08<\/strong><\/a>\u00a0|\u00a0<\/strong>\u00a0Vortex\u00a0<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>Pipet aid\u00a0<\/strong><\/a>\u00a0|\u00a0<\/strong>Petri dishes <\/strong>\u57f9\u990a\u76bf<\/strong><\/a><\/span><\/p>\n

\u98df\u54c1\u5fae\u751f\u7269\u4e4b\u6aa2\u9a57\u65b9\u6cd5<\/p>\n

Letheen Agar\u74ca\u8102| \u8ca8\u865fLAB185<\/a>\u00a0\u00a0(\u5375\u78f7\u8102)<\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Letheen\u74ca\u8102\u00a0(\u5375\u78f7\u8102)\u7528\u65bc\u8a55\u4f30\u5b63\u92a8\u5316\u5408\u7269(ammonium compounds)\u7684\u6bba\u83cc\u6d3b\u6027(bactericidal activity)\uff0c\u4e26\u8207Letheen Broth\u4e00\u8d77\u7528\u65bc\u78ba\u5b9a\u9632\u8150\u5291 (preservatives)\u5728\u5316\u599d\u54c1\u914d\u65b9\u4e2d\u7684\u9069\u7528\u6027(suitability)\uff0c\u5982\u7f8e\u570b\u6e2c\u8a66\u8207\u6750\u6599\u5b78\u6703\uff08ASTM\uff09\u6240\u898f\u5b9a\u7684\u542b\u6c34\u5316\u599d\u54c1\u4e2d\u7684\u9632\u8150\u5291\u3002Letheen\u74ca\u8102\u662f\u80f0\u86cb\u767dp\u8461\u8404\u7cd6\u63d0\u53d6\u7269\uff08 Tryptone Glucose Extract, TGE\uff09\u74ca\u8102\u7684\u4e00\u7a2e\u6539\u826f\u5291\uff0c\u914d\u88fd\u7528\u65bc\u4e2d\u548c\u7528\u65bc\u6e2c\u8a66\u6bba\u83cc\u6d3b\u6027\u7684\u5b63\u92a8\u5316\u5408\u7269\uff0cWeber\u548cBlack\u57281948\u5e74\u9996\u6b21\u63cf\u8ff0\u4e86\u5176\u91cd\u8981\u6027\u3002Letheen Agar(\u6dfb\u52a0Tween\u00ae80) \u53ef\u4ee5\u4e2d\u548c\u915aphenols\uff0c\u516d\u6c2f\u82efhexachlorophene\uff0c\u798f\u723e\u99ac\u6797formalin\u548c\u4e59\u9187ethanol\uff08\u5728\u5375\u78f7\u8102\u5b58\u5728\u4e0b\uff09\u3002\u7576\u8207hygiene swabbing protocol\u4e00\u8d77\u4f7f\u7528\u6642\uff0cLetheen Agar\u9084\u5141\u8a31\u8a55\u4f30\u83cc\u843d\u5f62\u6210\u55ae\u4f4d\u7684\u8a08\u7b97\uff0c\u4e26\u78ba\u4fdd\u4e0d\u6703\u5f9eswabbing diluent\/medium\u5e36\u8d70\u6d88\u6bd2\u5291\u3002<\/p>\n

Letheen\u74ca\u8102\u6210\u5206<\/p>\n

\u8461\u8404\u7cd6 (Dextrose )———————————————————- 1g \/ L<\/p>\n

Tryptone————————————————————————5g \/ L<\/p>\n

Beef extract——————————————————————–3g \/ L<\/p>\n

Lecithin ————————————————————————1g \/ L<\/p>\n

Polysorbate 80 —————————————————————7g \/ L<\/p>\n

Agar—————————————————————————15g \/ L<\/p>\n

\u57f9\u990a\u57fa\u4f7f\u7528\u65b9\u6cd5<\/strong>: <\/strong>\u5c0732\u514b<\/span>\u57f9\u990a\u57fa\u6eb6\u65bc1\u5347\u7d14\u6de8\u6c34\u4e2d\u3002\u5982\u6709\u5fc5\u8981\uff0c\u7d93\u5e38\u652a\u62cc\u52a0\u71b1\u4ee5\u5b8c\u5168\u6eb6\u89e3Medium\u3002\u5728121\u2103\u4e0b\u9ad8\u58d3\u6ec5\u83cc15\u5206\u9418\u3002\u7b49\u51b7\u5374\u5f8c\uff0c\u6700\u7d42pH\uff1a: 7.0 \u00b1 0.2, 25\u2103\u5206\u88dd\u65bcPetri dish\u57f9\u990a\u76bf\u4e2d\u3002<\/p>\n

\u6587\u737bReferences<\/p>\n

References American Society for Testing Materials. (1998). Standard Test Method for Preservatives in Water-Containing Cosmetics. E640-78.<\/p>\n

Annual Book of ASTM Standards, Philadelphia, PA. Association of Analytical Chemists. (1995). Official methods of analysis, 16th edition, section 6.Association of Official Analytical Chemists, Washington, D.C.<\/p>\n

Roberts, D., Hooper, W., and Greenwood, M. (1995). Methods for the examination of food for micro-organisms of public health significance, 2nd edition, section 5.10, Practical Food Microbiology. Butler & Tanner. ISBN 0 901144 36 3.<\/p>\n

Weber, G.R. and Black, L.A. (1948). Relative efficiencies of quaternary inhibitors. Soap and Sanit. Chem. 24: 134-139.<\/p>\n

\u66f4\u591a\u76f8\u95dc\u5546\u54c1 \u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a>| Agarose |\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a> | \u5fae\u751f\u7269\u6297\u751f\u7d20<\/a> | \u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a> |Mupid 2 DNA\u6c34\u6e96\u96fb\u6cf3\u69fd<\/a>| 3M\u5fae\u751f\u7269\u5feb\u901f\u6aa2\u6e2c\u7247<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

6\u516c\u5347\u8ff7\u4f60\u57f9\u990a\u7bb1 | \u83cc\u6db2\u6e2c\u91cf\u00a0(\u5206\u5149\u5149\u5ea6\u8a08)\u00a0\u00a0|\u00a0\u6ec5\u83cc\u91dc\u00a0 |\u00a0\u6c34\u6d74\u69fdWaterBath\u00a0|\u00a0\u96fb\u5b50\u5929\u5e73\u00a0| […]<\/p>\n","protected":false},"author":3,"featured_media":7149,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[475],"tags":[1857],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22375"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22375"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22375\/revisions"}],"predecessor-version":[{"id":22382,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22375\/revisions\/22382"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/7149"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22375"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22375"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22375"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}