{"id":22312,"date":"2019-10-14T11:32:49","date_gmt":"2019-10-14T03:32:49","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22312"},"modified":"2022-07-20T10:39:40","modified_gmt":"2022-07-20T02:39:40","slug":"%e4%b8%ad%e6%80%a7%e7%b4%85%e7%b4%b0%e8%83%9e%e7%b4%b0%e8%83%9e%e6%af%92%e6%80%a7%e6%b8%ac%e5%ae%9a-neutral-red-cell-cytotoxicity-assay-kit%e8%b2%a8%e8%99%9fk447-1000","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=22312","title":{"rendered":"\u4e2d\u6027\u7d05\u7d30\u80de\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a | Neutral Red Cell Cytotoxicity Assay Kit\u8ca8\u865fK447-1000"},"content":{"rendered":"

\u4e2d\u6027\u7d05\u7d30\u80de\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a | <\/strong>\u8ca8\u865f<\/strong>K447-1000<\/a><\/span><\/p>\n

Neutral Red Cell Cytotoxicity Assay Kit<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u4e2d\u6027\u7d05\u7d30\u80de\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a(Neutral red cell cytotoxicity assay)\u662f\u7528\u65bc\u6aa2\u6e2c\u7d30\u80de\u6d3b\u529b(cell viability)\u6216\u85e5\u7269\u7d30\u80de\u6bd2\u6027(drug cytotoxicity)\u7684\u5e38\u7528\u65b9\u6cd5\u4e4b\u4e00\u3002 \u8a72\u6e2c\u5b9a\u7684\u539f\u7406\u662f\u57fa\u65bc\u901a\u904e\u651d\u53d6\u67d3\u6599\u4e2d\u6027\u7d05(neutral red)\u4f86\u6aa2\u6e2c\u6d3b\u7d30\u80de\u3002\u4e2d\u6027\u7d05\u662f\u4e00\u7a2e\u771f\u6838\u751f\u7269\u67d3\u6599(eurhodin dye)\uff0c\u53ef\u67d3\u8272\u6d3b\u7d30\u80de\u4e2d\u7684\u6eb6\u9176\u9ad4(lysosomes)\u3002\u6d3b\u7d30\u80de\u53ef\u4ee5\u901a\u904e\u4e3b\u52d5\u8f49\u904b\u5438\u6536\u4e2d\u6027\u7d05\uff0c\u4e26\u5c07\u67d3\u6599\u647b\u5165\u5176\u6eb6\u9176\u9ad4(lysosomes)\u4e2d\uff0c\u4f46\u975e\u6d3b\u7d30\u80de\u4e0d\u80fd\u5438\u6536\u8a72\u751f\u8272\u5718(chromophore)\u3002\u56e0\u6b64\uff0c\u5728\u6d17\u6ecc\u5f8c\uff0c\u6d3b\u7d30\u80de\u53ef\u4ee5\u5728\u9178\u5316\u63d0\u53d6\u7684\u689d\u4ef6\u4e0b\u91cb\u653e\u647b\u5165\u7684\u67d3\u6599\u3002\u91cb\u653e\u7684\u67d3\u6599\u91cf\u53ef\u7528\u65bc\u78ba\u5b9a\u6d3b\u7d30\u80de\u7e3d\u6578\u6216\u85e5\u7269\u7d30\u80de\u6bd2\u6027\u3002\u4e2d\u6027\u7d05\u5438\u6536\u6e2c\u5b9a\u6cd5\u63d0\u4f9b\u4e86\u6d3b\u7d30\u80de\u6578\u91cf\u7684\u5b9a\u91cf\u6e2c\u91cf\uff0c\u53ef\u4ee5\u5728OD 540 nm\u8655\u6e2c\u91cf\u3002 BioVision\u7684\u4e2d\u6027\u7d05\u7d30\u80de\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u7c21\u55ae\uff0c\u6e96\u78ba\u4e14\u53ef\u91cd\u8907\u3002\u5b83\u9084\u5305\u62ec\u963f\u9ef4\u7d20(Doxorubicin)\uff0c\u4f5c\u70ba\u967d\u6027\u5c0d\u7167(positive control)\u3002\u8a72\u8a66\u5291\u76d2\u70ba\u9ad4\u5916\u7d30\u80de\u6bd2\u6027\u7814\u7a76\u4ee5\u53ca\u9ad8\u901a\u91cf\u85e5\u7269\u7be9\u9078\u63d0\u4f9b\u4e86\u4e00\u7a2e\u51fa\u8272\u800c\u6709\u6548\u7684\u65b9\u6cd5\uff0c\u6bcf\u5b54\u53ef\u6aa2\u6e2c\u52305,000-50,000\u500b\u7d30\u80de\u3002<\/p>\n

\"\"\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK447-1000<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n1000 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Absorbance (540 nm)
\n\u2022 Sample type- Cell culture (adherent and non adherent
\n\u2022 Species reactivity- Mammalian
\n\u2022 Applications- This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of neutral cells to stain lysosomes of viable cells.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nDetection method- Absorbance (540 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThis assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of neutral cells to stain lysosomes of viable cells.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes less than 2 hours
\n\u2022 Fast, sensitive and convenient<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 100X Neutral Red Staining Solution
\n\u2022 10X Washing Solution
\n\u2022 2X Solubilization Solution
\n\u2022 20 mM Doxorubicin<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u4e2d\u6027\u7d05\u7d30\u80de\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a | \u8ca8\u865fK447-1000 Neutral Red Cell Cytotoxicity […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1874],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22312"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22312"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22312\/revisions"}],"predecessor-version":[{"id":22313,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22312\/revisions\/22313"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22312"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22312"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22312"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}