{"id":22262,"date":"2019-10-03T15:04:37","date_gmt":"2019-10-03T07:04:37","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22262"},"modified":"2022-07-20T10:41:04","modified_gmt":"2022-07-20T02:41:04","slug":"mouse-anti-human-ige-fc-fitc%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f9250-02","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=22262","title":{"rendered":"Mouse Anti-Human IgE Fc-FITC\u6297\u9ad4 | \u8ca8\u865f9250-02"},"content":{"rendered":"

Mouse Anti-Human IgE Fc-FITC\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong>9250-02<\/strong><\/a>\u00a0\u00a0<\/span>\u898f\u683c<\/strong>1.0 ml \u00a0(0.5 mg\/mL)<\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\uff0c\u76ee\u524d\u9664\u4e86\u4e00\u822c\u5e38\u898b\u7684\u591a\u682a\u8207\u55ae\u682a\u4e8c\u7d1a\u6297\u9ad4\u5916\uff0c\u9084\u5e36\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u3001\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001Low Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\uff0c\u4ee5\u53caF(ab)\u548cF(ab\u2019)2\u7b49\u6297\u9ad4\u7247\u6bb5\u3002\u8af8\u5982\u6b64\u985e\u591a\u6a23\u5316\u7522\u54c1\uff0c\u5354\u52a9\u5404\u7a2e\u5be6\u9a57\u9032\u884c\u3002<\/p>\n

\u4e8c\u7d1a\u6297\u9ad4 (Secondary antibodies) \u61c9\u7528:<\/p>\n

\u4e8c\u6297(Secondary antibodies)\u662f\u91dd\u5c0d\u514d\u75ab\u7403\u86cb\u767d\uff08immunoglobulin , Ig\uff09\u5206\u5b50\u7684\u591a\u514b\u9686(polyclonal) \u548c\u55ae\u514b\u9686\u6297\u9ad4(monoclonal antibodies)\u3002\u5b83\u5011\u901a\u5e38\u8207\u6297\u539f\u7279\u7570\u6027\u4e00\u6297(specific primary antibody) \u4e00\u8d77\u4f7f\u7528\uff0c\u4e26\u4e14\u901a\u5e38\u88ab\u6a19\u5b9a\u9175\u7d20\u6216\u87a2\u5149\u7269\u8cea\u4ee5\u5e6b\u52a9\u6aa2\u6e2c\u3002\u4e8c\u6297\u53ef\u7528\u65bc\u591a\u7a2e\u61c9\u7528\uff0c\u4f8b\u5982ELISA\u3001\u514d\u75ab\u5370\u8de1(Immunoblotting)\u3001\u6d41\u5f0f\u7d30\u80de\u5100(Flow Cytometry)\u3001\u514d\u75ab\u7d44\u7e54\u67d3\u8272(Immunohistochemistry)\u3001\u514d\u75ab\u7d30\u80de\u5316\u5b78(Immuocytochemistry)\u3002\u4e8c\u7d1a\u6297\u9ad4\u901a\u5e38\u7528\u9176\u6216\u87a2\u5149\u67d3\u6599\u6a19\u8a18\uff0c\u9019\u4f7f\u5f97\u80fd\u5920\u901a\u904e\u6bd4\u8272\uff0c\u5316\u5b78\u767c\u5149\u548c\u87a2\u5149\u6280\u8853\u9032\u884c\u6aa2\u6e2c\u3002\u4f8b\u5982\uff0c\u9176\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\uff08enzymes horseradish peroxidase , HRP\uff09\u548c\u9e7c\u6027\u78f7\u9178\u9176\uff08alkaline phosphatase , AP\uff09\u901a\u904e\u6bd4\u8272\u548c\u5316\u5b78\u767c\u5149\u5e73\u53f0\u5728ELISA\u548c\u86cb\u767d\u8cea\u5370\u8de1\u61c9\u7528\u4e2d\u662f\u6709\u50f9\u503c\u7684\u3002\u87a2\u5149\u86cb\u767d\u8af8\u5982\u87a2\u5149\u7d20\uff08fluorescein , FITC\uff09\uff0c\u85fb\u7d05\u86cb\u767d\uff08phycoerythrin , PE\uff09\u548c\u82b1\u9752\uff08cyanine , CY\uff09\u7684\u87a2\u5149\u67d3\u6599\u5728\u87a2\u5149\u7684\u61c9\u7528\u4e2d\u662f\u5fc5\u4e0d\u53ef\u5c11\u7684\uff0c\u5305\u62ec\u6d41\u5f0f\u7d30\u80de\u8853\uff0c\u514d\u75ab\u87a2\u5149\u986f\u5fae\u8853\u548c\u591a\u91cd\u6e2c\u5b9a\u3002\u4e8c\u6297\u5141\u8a31\u6a19\u8a18\u548c\u6aa2\u6e2c\u7684\u9748\u6d3b\u6027\u4ee5\u53ca\u901a\u904e\u4fe1\u865f\u653e\u5927\uff0c\u53ef\u589e\u52a0\u7684\u9748\u654f\u5ea6\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0Mouse Anti-Human IgE Fc-FITC\u6297\u9ad4\uff0c\u5177FITC\u6a19\u8a8c\u3002\u514d\u75ab\u6e90\u70baPS human IgE myeloma protein\u91dd\u5c0dHuman IgE Fc\u5177\u7279\u7570\u6027\u3002Isotype\u70baMouse IgG2a<\/sub>\u03ba\u3002Isotype Control Mouse IgG2a<\/sub>-FITC<\/a><\/span>\u3002 \u7de9\u885d\u6db2\u914d\u65b9\u5728Phosphate buffered saline \u542b\u6709 0.1% sodium azide\u9632\u8150\u5291\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\nHP6029<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nMouse IgG2a<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nMouse IgG2a<\/sub>-FITC<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nPS human IgE myeloma protein<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nHuman IgE Fc<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nFITC (Fluorescein)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nPhosphate buffered saline containing 0.1% sodium azide<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n0.5 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C; Avoid exposure to light
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

ELISA \u2013 Quality tested\u00a01-8,11<\/sup>
\nFLISA \u2013 Quality tested
\nELISpot \u2013 Reported in literature\u00a05<\/sup>
\nWestern Blot \u2013 Reported in literature\u00a09,10<\/sup>
\nPurification \u2013 Reported in literature\u00a011<\/sup><\/td>\n<\/tr>\n

Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2796717<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

SBA<\/strong>\u71b1\u92b7\u5546\u54c1<\/strong>\u00a0\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a>\u00a0|\u00a0monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0|\u00a0Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a><\/u>\u00a0|\u00a0<\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|<\/u>\u00a0<\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/u>|\u00a0<\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0| <\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a>| \u4e00\u7d1a\u6297\u9ad4<\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
\u00a0Cat. No.<\/strong><\/td>\nProduct Description<\/strong><\/td>\nClone<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n<\/thead>\n
9250-04<\/strong><\/a><\/td>\nMouse Anti-Human IgE Fc-AP<\/strong><\/td>\nHP6029<\/td>\n1.0 mL<\/td>\n<\/tr>\n
9250-08<\/strong><\/a><\/td>\nMouse Anti-Human IgE Fc-BIOT<\/strong><\/td>\nHP6029<\/td>\n0.5 mg<\/td>\n<\/tr>\n
9250-02<\/strong><\/a><\/td>\nMouse Anti-Human IgE Fc-FITC<\/strong><\/td>\nHP6029<\/td>\n0.5 mg<\/td>\n<\/tr>\n
9250-05<\/strong><\/a><\/td>\nMouse Anti-Human IgE Fc-HRP<\/strong><\/td>\nHP6029<\/td>\n1.0 mL<\/td>\n<\/tr>\n
9250-01<\/strong><\/a><\/td>\nMouse Anti-Human IgE Fc-UNLB<\/strong><\/td>\nHP6029<\/td>\n0.5 mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

References<\/p>\n

    \n
  1. Agea E, Russano A, Bistoni O, Mannucci R, Nicoletti I, Corazzi L, et al. Human CD1-restricted T cell recognition of lipids from pollens. J Exp Med. 2005;202:295-308. (ELISA)<\/li>\n
  2. Milovanovic M, Heine G, Zuberbier T, Worm M. Allergen extract-induced interleukin-10 in human memory B cells inhibits immunoglobulin E production. Clin Exp Allergy. 2009;39:671-8. (ELISA)<\/li>\n
  3. Heine G, Dahten A, Hilt K, Ernst D, Milovanovic M, Hartmann B, et al. Liver X receptors control IgE expression in B cells. J Immunol. 2009;182:5276-82. (ELISA)<\/li>\n
  4. Milovanovic M, Drozdenko G, Weise C, Babina M, Worm M. Interleukin-17A promotes IgE production in human B cells. J Invest Dermatol. 2010;130:2621-8. (ELISA)<\/li>\n
  5. Weise C, Hilt K, Milovanovic M, Ernst D, R\u00fchl R, Worm M. Inhibition of IgE production by docosahexaenoic acid is mediated by direct interference with STAT6 and NF\u03baB pathway in human B cells. J Nutr Biochem. 2011;22:269-75. (ELISA, ELISPOT)<\/li>\n
  6. Sabbatini P, Tsuji T, Ferran L, Ritter E, Sedrak C, Tuballes K, et al. Phase I trial of overlapping long peptides from a tumor self-antigen and poly-ICLC shows rapid induction of integrated immune response in ovarian cancer patients. Clin Cancer Res. 2012;18:6497-508. (ELISA)<\/li>\n
  7. Qian Y, Jeong JS, Abdeladhim M, Valenzuela JG, Aoki V, Hans-Filhio G, et al. IgE anti-LJM11 sand fly salivary antigen may herald the onset of Fogo selvagem in endemic Brazilian regions. J Invest Dermatol. 2015;135:913-5. (ELISA)<\/li>\n
  8. Bundhoo A, Paveglio S, Rafti E, Dhongade A, Blumberg RS, Matson AP. Evidence that FcRn mediates the transplacental passage of maternal IgE in the form of IgG anti-IgE\/IgE immune complexes. Clin Exp Allergy. 2015 Feb 5. doi: 10.1111\/cea.12508. [Epub ahead of print]. (ELISA)<\/li>\n
  9. Martos G, Pineda-Vadillo C, Miralles B, Alonso-Lebrero E, L\u00f3pez-Fandi\u00f1o R, Molina E, et al. Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion. J Agric Food Chem. 2012;60:5215-20. (WB)<\/li>\n
  10. Bened\u00e9 S, L\u00f3pez-Exp\u00f3sito I, Gim\u00e9nez G, Grishina G, Bardina L, Sampson HA, et al. In vitro digestibility of bovine \u03b2-casein with simulated and human oral and gastrointestinal fluids. Identification and IgE-reactivity of the resultant peptides. Food Chem. 2014;143:514-21. (WB)<\/li>\n
  11. Kleine-Tebbe J, Hamilton RG, Roebber M, Lichtenstein LM, MacDonald SM. Purification of immunoglobulin E (IgE) antibodies from sera with high IgE titers. J Immunol Methods. 1995;179:153-64. (Purification, ELISA)<\/li>\n<\/ol>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Mouse Anti-Human IgE Fc-FITC\u6297\u9ad4 | \u8ca8\u865f9250-02\u00a0\u00a0\u898f\u683c1.0 ml \u00a0( […]<\/p>\n","protected":false},"author":3,"featured_media":6176,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[1885],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22262"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22262"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22262\/revisions"}],"predecessor-version":[{"id":22267,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22262\/revisions\/22267"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6176"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22262"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22262"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22262"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}