{"id":22021,"date":"2019-09-23T10:10:09","date_gmt":"2019-09-23T02:10:09","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=22021"},"modified":"2022-07-20T10:44:59","modified_gmt":"2022-07-20T02:44:59","slug":"luciferase-firefly-lentiviral-vector-cmv%e6%85%a2%e7%97%85%e6%af%92%e8%a1%a8%e7%8f%be%e8%bc%89%e9%ab%94-%e8%b2%a8%e8%99%9flv010162","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=22021","title":{"rendered":"LUCIFERASE (FIREFLY) Lentiviral Vector (CMV)\u6162\u75c5\u6bd2\u8868\u73fe\u8f09\u9ad4 | \u8ca8\u865fLV010162"},"content":{"rendered":"

LUCIFERASE (FIREFLY) Lentiviral Vector (CMV)<\/strong>\u6162\u75c5\u6bd2\u8868\u73fe\u8f09\u9ad4 | \u8ca8\u865fLV010162<\/a><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0<\/strong>:<\/strong> pLenti-III-mKate2-2A-luc (CMV promoter) (<\/u>Gene Insert <\/strong>Luciferase (Firefly) \u67b6\u69cb\u65bcpLenti-III-CMV<\/a><\/span>\u8868\u73fe\u8f09\u9ad4\u4e0a\uff0c\u5177\u6709Luciferase\u57fa\u56e0\uff0c\u69cb\u6210pLenti-III-mKate2-2A-luc (CMV promoter)\u3002\u6162\u75c5\u6bd2\u8f09\u9ad4\uff0c\u53ef\u7d50\u5408packaging mix (\u6539\u826f\u5f0f\u00a0<\/strong>\u75c5\u6bd2\u5305\u88dd\u7cfb\u7d71\u00a0<\/strong><\/a><\/span>LV003)<\/span> <\/strong>\u4ee5\u5728\u591a\u7a2e\u5bbf\u4e3b\u7d30\u80de\u6216\u52d5\u7269\u6a21\u578b\u4e2d\u904e\u8868\u9054\u3002 \u8a72\u69cb\u5efa\u9ad4\u542b\u6709\u7528\u65bc\u76e3\u6e2c\u8f49\u67d3\u6548\u7387\u7684Luciferase\u57fa\u56e0\uff0c\u53ef\u4ee5Monitor\u8f49\u67d3\u6548\u7387\u3002\u53ef\u76f4\u63a5\u4ee5\u975e\u75c5\u6bd2\u8cea\u7c92\u8f49\u67d3 (non-viral plasmid transfection) \u7acb\u5373\u8868\u9054\uff0c\u6216\u7d93\u7531\u5305\u88dd\u6210\u6162\u75c5\u6bd2\u9846\u7c92\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Description<\/strong><\/td>\nmKate2 linked to a firefly Luciferase reporter by a 2A peptide, expression driven by CMV promoter.<\/td>\n<\/tr>\n
Product Application<\/strong><\/td>\n\n

\u00b7\u00a0 Direct non-viral plasmid transfection for immediate expression<\/p>\n

\u00b7\u00a0 Package into Lentiviral particles for high efficiency and stably integrated expression<\/td>\n<\/tr>\n

Gene Insert<\/strong><\/td>\nLuciferase (Firefly)<\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npLenti-III-CMV<\/a><\/td>\n<\/tr>\n
Vector Size<\/strong><\/td>\n10506 bp<\/td>\n<\/tr>\n
Insert Size<\/strong><\/td>\n0 bp<\/td>\n<\/tr>\n
Reporter\/Tag<\/strong><\/td>\nNo Tags<\/td>\n<\/tr>\n
Promoter<\/strong><\/td>\nCMV<\/td>\n<\/tr>\n
Selection Marker<\/strong><\/td>\nBacterial: Kanamycin; Mammalian: Puromycin<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nPlasmid<\/td>\n<\/tr>\n
Viral Packaging<\/strong><\/td>\nabm<\/strong>\u00a0recommends using Cat#\u00a0G074, Lentifectin\u2122<\/a>\u00a0in conjunction with one of the following packaging mixes. The suggested packaging mix for this vector is Cat#\u00a0LV003, 2nd Generation Packaging Mix<\/a>. Alternatively, you can also use Cat#\u00a0LV053, 3rd Generation Packaging Mix<\/a><\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nClear Liquid<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Stability<\/strong><\/td>\n1 year when stored at -20\u00b0C or lower in a non-frost free freezer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/p>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

LUCIFERASE (FIREFLY) Lentiviral Vector (CMV)\u6162\u75c5\u6bd2\u8868\u73fe\u8f09\u9ad4 | \u8ca8 […]<\/p>\n","protected":false},"author":3,"featured_media":2639,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1909],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22021"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22021"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22021\/revisions"}],"predecessor-version":[{"id":22025,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/22021\/revisions\/22025"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/2639"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22021"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22021"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22021"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}