{"id":21987,"date":"2019-09-18T17:39:29","date_gmt":"2019-09-18T09:39:29","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21987"},"modified":"2022-07-20T10:46:17","modified_gmt":"2022-07-20T02:46:17","slug":"mouse-anti-his-tag-hrp-%e7%be%8e%e5%9c%8b%e6%8a%97%e9%ab%94%e7%94%9f%e7%94%a2%e5%95%86southern-biotech%e8%b2%a8%e8%99%9f4603-05","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=21987","title":{"rendered":"Mouse Anti-His-Tag-HRP |\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u8ca8\u865f4603-05"},"content":{"rendered":"

Mouse Anti-His-Tag-HRP\u6297\u9ad4\u00a0|\u00a0<\/strong>\u8ca8\u865f<\/strong><\/a><\/span>4603-05\u00a0\u00a0<\/a><\/span>\u00a0\u00a0<\/strong>\u898f\u683c 1<\/strong>mL<\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\uff0c\u76ee\u524d\u9664\u4e86\u4e00\u822c\u5e38\u898b\u7684\u591a\u682a\u8207\u55ae\u682a\u4e8c\u7d1a\u6297\u9ad4\u5916\uff0c\u9084\u5e36\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u3001\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001Low Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\uff0c\u4ee5\u53caF(ab)\u548cF(ab\u2019)2\u7b49\u6297\u9ad4\u7247\u6bb5\u3002\u8af8\u5982\u6b64\u985e\u591a\u6a23\u5316\u7522\u54c1\uff0c\u5354\u52a9\u5404\u7a2e\u5be6\u9a57\u9032\u884c\u3002<\/p>\n

\"\"<\/p>\n

ELISA plate was coated with N-terminal 6X His-tag conjugated to BSA and BSA. His-tag was detected with serially diluted Mouse Anti-His-Tag-HRP (SB Cat. No. 4603-05).<\/p>\n

\u8a31\u591a\u8cea\u9ad4DNA\u542b\u6709\u7de8\u78bc\u516d\u500b\u7d44\u6c28\u9178\uff08His\uff09\u6b98\u57fa\u7d44\u6210\u7684N-\u672b\u7aef\u6a19\u7c64\u7684DNA\uff0c\u53ca\u64f4\u5c55\u7684extended multiple cloning sites\u591a\u514b\u9686\u4f4d\u9ede\u3002 \u8868\u9054\u7684\u91cd\u7d44\u86cb\u767d\u4e0a\u76846xHis\u6a19\u7c64\u5141\u8a31\u6709\u6548\u5076\u806f(coupling to) \u81f3Ni ++\u89aa\u548c\u6a39\u8102(Ni++ affinity resins)\u4e26\u901a\u904e\u55ae\u6b65\u8272\u8b5c\u6cd5(single step chromatography)\u7d14\u5316\u3002\u8207\u5176\u4ed6\u86cb\u767d\u8cea\u6a19\u7c64(protein tags)\u7684\u60c5\u6cc1\u4e00\u6a23\uff0c\u8a72\u591a\u7d44\u6c28\u9178\u6a19\u7c64(polyhistidine tag)\u53ef\u4ee5\u5728\u9176\uff08\u4f8b\u5982\u51dd\u8840\u9176(thrombin)\u548c\u8178\u6fc0\u9176(enterokinases)\uff09\u8b58\u5225\u7684\u4f4d\u9ede\u8655\u88ab\u5207\u5272\u4ee5\u5206\u96e2\u76ee\u7684\u86cb\u767d\u8cea\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\nSB194b<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nMouse (BALB\/c) IgG1<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nMouse IgG1<\/sub>-HRP<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nSynthetic 6X His-tag peptides<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nHis-tag
\nReacts with N-terminal, C-terminal, and internal repeating histidine residues of 6 or more<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nHRP (Horseradish Peroxidase)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\n50% Glycerol\/50% Phosphate buffered saline, pH 7.4<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

ELISA \u2013 Quality tested\u00a01<\/sup>
\nFLISA \u2013 Quality tested\u00a01<\/sup>
\nImmunoprecipitation \u2013 Quality tested\u00a01<\/sup>
\nWestern blot\u00a01<\/sup>
\nPurification\u00a01<\/sup>
\nFlow Cytometry\u00a01<\/sup>
\nImmunocytochemistry\u00a01<\/sup><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Human peripheral blood lymphocytes were stained with purified \/ unlabeled His-tagged recombinant Anti-Human IgM followed by Mouse Anti-His-Tag-AF488 (SB Cat. No. 4603-30).<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong>\u00a0 Mouse Anti-His-Tag-HRP\u6297\u9ad4\uff0c\u5177HRP\u6a19\u8a8c\u3002\u514d\u75ab\u6e90Synthetic 6X His-tag peptides\u91dd\u5c0dHis-tag (Reacts with N-terminal, C-terminal, and internal repeating histidine residues of 6 or more)\u5177\u7279\u7570\u6027\u3002Isotype\u70baMouse (BALB\/c) IgG1<\/sub>\u03ba\u3002Isotype Control Mouse IgG1<\/sub>-HRP<\/a>\u3002 \u7de9\u885d\u6db2\u914d\u65b9\u5728Phosphate buffered saline\u542b\u67090.1% sodium azide\u9632\u8150\u5291\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0<\/strong>Cat. No.<\/td>\nProduct Description<\/td>\nClone<\/td>\nSize<\/td>\n<\/tr>\n<\/thead>\n
4603-30<\/a><\/td>\nMouse Anti-His-Tag-Alexa Fluor\u00ae<\/sup>\u00a0488<\/td>\nSB194b<\/td>\n0.1 mg<\/td>\n<\/tr>\n
4603-31<\/a><\/td>\nMouse Anti-His-Tag-Alexa Fluor\u00ae<\/sup>\u00a0647<\/td>\nSB194b<\/td>\n0.1 mg<\/td>\n<\/tr>\n
4603-08<\/a><\/td>\nMouse Anti-His-Tag-BIOT<\/td>\nSB194b<\/td>\n0.5 mg<\/td>\n<\/tr>\n
4603-05<\/a><\/td>\nMouse Anti-His-Tag-HRP<\/td>\nSB194b<\/td>\n1.0 mL<\/td>\n<\/tr>\n
4603-25<\/a><\/td>\nMouse Anti-His-Tag-SEPH<\/td>\nSB194b<\/td>\n25 tests<\/td>\n<\/tr>\n
4603-01L<\/a><\/td>\nMouse Anti-His-Tag-UNLB<\/td>\nSB194b<\/td>\n1.0 mg<\/td>\n<\/tr>\n
4603-01M<\/a><\/td>\nMouse Anti-His-Tag-UNLB<\/td>\nSB194b<\/td>\n0.5 mg<\/td>\n<\/tr>\n
4603-01S<\/a><\/td>\nMouse Anti-His-Tag-UNLB<\/td>\nSB194b<\/td>\n0.1 mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0SBA<\/strong>\u71b1\u92b7\u5546\u54c1<\/strong><\/span>\u00a0\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a>\u00a0|\u00a0monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0|\u00a0Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a><\/u>\u00a0|\u00a0<\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|<\/u>\u00a0<\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/u>|\u00a0<\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0|\u00a0<\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a>|\u00a0\u4e00\u7d1a\u6297\u9ad4<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Mouse Anti-His-Tag-HRP\u6297\u9ad4\u00a0|\u00a0\u8ca8\u865f4603-05\u00a0\u00a0\u00a0\u00a0\u898f\u683c 1mL \u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546S […]<\/p>\n","protected":false},"author":3,"featured_media":18700,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[1921],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21987"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21987"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21987\/revisions"}],"predecessor-version":[{"id":21992,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21987\/revisions\/21992"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/18700"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21987"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21987"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21987"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}