{"id":21605,"date":"2019-08-07T13:22:14","date_gmt":"2019-08-07T05:22:14","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21605"},"modified":"2022-07-20T10:58:45","modified_gmt":"2022-07-20T02:58:45","slug":"tp53-sgrna-crispr%e6%85%a2%e7%97%85%e6%af%92-tp53-sgrna-crispr-all-in-one-lentivirus-set%e8%b2%a8%e8%99%9fk0005315","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=21605","title":{"rendered":"TP53 sgRNA CRISPR\u6162\u75c5\u6bd2| TP53 sgRNA CRISPR All-in-One Lentivirus set\u8ca8\u865fK0005315"},"content":{"rendered":"

TP53 sgRNA CRISPR\u6162\u75c5\u6bd2<\/strong>| <\/strong>\u8ca8\u865f<\/strong><\/a><\/span>K0005315 <\/a><\/span>\u00a0<\/strong>3 x 300 \u00b5l<\/p>\n

TP53 sgRNA CRISPR All-in-One Lentivirus set (Human)<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>: <\/strong>TP53 sgRNA CRISPR All-in-One Lentivirus set (Human)<\/u>\u6162\u75c5\u6bd2, \u529b\u50f9>1×10\u2077IU\/ml\u3002TP53<\/u> (Accession Number \u00a0<\/strong>NM_000546 ; NM_001276760<\/a>) \u67b6\u69cb\u65bc
\npLenti-U6-sgRNA-SFFV-Cas9-2A-Puro<\/span><\/a> (Human)\u3002Cas9 Promoter\u555f\u52d5\u5b50: Cas9\u6838\u9178\u9176\u53d7SFFV\u555f\u52d5\u5b50\u7684\u63a7\u5236\uff0c\u9664\u4e86ES\u7d30\u80de\u6216iPS\u7d30\u80de\u5916\uff0c\u5b83\u5c0d\u7d55\u5927\u591a\u6578\u7d30\u80de\u90fd\u6709\u6548\u3002 \u53e6\u6709EF1a\u555f\u52d5\u5b50\u66ff\u63dbSFFV\u555f\u52d5\u5b50\u4f5c\u70ba\u5ba2\u88fd\u5316\u670d\u52d9\u3002\u53ef\u76f4\u63a5\u4ee5\u975e\u75c5\u6bd2\u8cea\u7c92\u9032\u884c\u8f49\u67d3\u7528\u65bc\u5373\u6642\u8868\u9054\u3002\u6162\u75c5\u6bd2\u5305\u88dd\u7cfb\u5217\u7522\u54c1\u5305\u62ecLentivirus<\/u>\u6162\u75c5\u6bd2\u4f4e\u901f\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>\/<\/span>\u00a0Lentivirus <\/u>\u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a><\/span>\u4e26\u4ee5\u00a0Lentivirus<\/u>\u75c5\u6bd2<\/u>RT-QPCR<\/u>\u5b9a\u91cf<\/u>(\u5b9a\u91cf)<\/a>\u00a0<\/span>\u7d14\u5316\u5f8c\u4e4b\u75c5\u6bd2\u529b\u50f9\u3002<\/p>\n

TP53 sgRNA CRISPR All-in-One Lentivirus set (Human)<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Accession Number<\/strong><\/td>\nNM_000546 ; NM_001276760<\/a><\/td>\n<\/tr>\n
Version<\/strong><\/td>\nNM_000546 ; NM_001276760<\/td>\n<\/tr>\n
Procedure<\/strong><\/td>\n<\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npLenti-U6-sgRNA-SFFV-Cas9-2A-Puro<\/a><\/td>\n<\/tr>\n
Cas9 Promoter<\/strong><\/td>\nCas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. If necessary, the SFFV promoter can be replaced with EF1a promoter as a custom cloning service. Please inquire for pricing.<\/td>\n<\/tr>\n
Target Sequence<\/strong><\/td>\n23 CCATTGTTCAATATCGTCCG
\n111 ACCAGCAGCTCCTACACCGG
\n195 GACGGAAACCGTAGCTGCCC<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nVirus<\/td>\n<\/tr>\n
Titer<\/strong><\/td>\n>1×10\u2077IU\/ml<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nLentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80\u00b0C in small aliquots to avoid repeated freeze-thaw cycles.<\/td>\n<\/tr>\n
Shelf Life<\/strong><\/td>\n1 year (when at -80\u00b0C or below in a non-frost free freezer)<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nShipped with dry ice<\/td>\n<\/tr>\n
Additional Materials<\/strong><\/td>\n–\u00a0CRISPR Genomic Cleavage Detection Kit (G932)<\/a>
\n–\u00a0sgRNA CRISPR\/Cas9 All-in-One Lentivirus Control (K011)<\/a><\/td>\n<\/tr>\n
Quality Control<\/strong><\/td>\nRestriction Enzyme Digest and Sequencing<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6162\u75c5\u6bd2\u6280\u8853\u4e4b\u9818\u5c0e\u8005abm\uff0c\u958b\u767c\u4e86\u4e00\u5957\u5168\u9762\u7684\u4eba\u985e\u3001\u5c0f\u9f20\u548c\u5927\u9f20\u57fa\u56e0\u6587\u5eab\uff0cClone\u5230\u6162\u75c5\u6bd2\u8f09\u9ad4\u6216\u88fd\u6210\u5373\u7528\u578b\u6162\u75c5\u6bd2\uff0c\u53ef\u7528\u65bc\u64cd\u7e31\u611f\u8208\u8da3\u57fa\u56e0\u65bc\u5bbf\u4e3b\u7d30\u80de\u8868\u9054\u3002\u70ba\u4e86\u4fc3\u9032\u6211\u5011\u7684\u6162\u75c5\u6bd2\u8f09\u9ad4\u7684\u61c9\u7528\u548c\u591a\u529f\u80fd\u6027\uff0cABM\u6162\u75c5\u6bd2\u8868\u9054\u7cfb\u7d71\u5177\u6709\u8a31\u591a\u7d44\u6210\u578b\u555f\u52d5\u5b50\uff0c\u6a19\u7c64\u5e8f\u5217\u548c\u5831\u5c0e\u57fa\u56e0\u4e4b\u7d44\u5408\u3002<\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

TP53 sgRNA CRISPR\u6162\u75c5\u6bd2| \u8ca8\u865fK0005315 \u00a03 x 300 \u00b5l TP53 sgRNA […]<\/p>\n","protected":false},"author":3,"featured_media":21606,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[522],"tags":[1983],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21605"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21605"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21605\/revisions"}],"predecessor-version":[{"id":21608,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21605\/revisions\/21608"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/21606"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21605"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21605"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21605"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}