{"id":21579,"date":"2019-08-02T14:58:53","date_gmt":"2019-08-02T06:58:53","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21579"},"modified":"2022-07-20T11:00:18","modified_gmt":"2022-07-20T03:00:18","slug":"ldha-%e4%ba%ba%e9%a1%9e%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-recombinant-protein-%e8%b2%a8%e8%99%9f6374-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=21579","title":{"rendered":"LDHA-\u4eba\u985e\u91cd\u7d44\u86cb\u767d Recombinant Protein | \u8ca8\u865f6374-100"},"content":{"rendered":"

LDHA-\u4eba\u985e\u91cd\u7d44\u86cb\u767d Recombinant Protein | <\/strong>\u8ca8\u865f<\/strong>6374-100<\/a><\/span><\/p>\n

Catalyzes the final step of anaerobic glycolysis<\/em>\u00a0<\/em><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u5728\u7121\u6c27\u7cd6\u9175\u89e3(anaerobic glycolysis)\u7684\u6700\u5f8c\u6b65\u9a5f\u4e2d\uff0cLDHA\u50ac\u5316L-\u4e73\u9178(L-lactate)\u548cNAD\u8f49\u5316\u4e19\u916e\u9178(pyruvate)\u548cNADH\u3002 LDHA\u4e3b\u8981\u4f4d\u65bc\u808c\u8089\u7d44\u7e54(muscle tissue)\u4e2d\uff0c\u662f\u4e73\u9178\u812b\u6c2b\u9176\u5bb6\u65cf(lactate dehydrogenase family)\u7684\u4e00\u90e8\u5206\u3002 LDHA\u7684\u7a81\u8b8a(Mutations)\u8207\u52de\u529b\u6027\u808c\u7d05\u86cb\u767d\u5c3f(exertional myoglobinuria)\u6709\u95dc\u3002 LDH1\u5728\u539f\u767c\u6027\u8840\u5c0f\u677f\u589e\u591a\u75c7(thrombocythemia)\u4e2d\u6e1b\u5c11\u3002 LDHA\u901a\u904e\u96cc\u6fc0\u7d20(estrogen)\u4f5c\u7528\u7684\u975e\u57fa\u56e0\u7d44(non-genomic pathway)\u9014\u5f91\u8a98\u5c0e\u3002 LDH-A\u6d3b\u6027\u7684\u964d\u4f4e\u5c0e\u81f4\u7dda\u7c92\u9ad4\u547c\u5438\u7684\u523a\u6fc0\u548c\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u7684\u964d\u4f4e\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\n6374-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 \u03bcg<\/td>\n<\/tr>\n
Alternate Name<\/strong><\/td>\nL-lactate dehydrogenase A chain, GSD11, LDH1, LDHM, PIG19.<\/td>\n<\/tr>\n
Gene Symbol<\/strong><\/td>\nLDHA<\/td>\n<\/tr>\n
Gene ID<\/strong><\/td>\n3939<\/td>\n<\/tr>\n
Accession #<\/strong><\/td>\nP00338<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nE. Coli<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Physical Form Description<\/strong><\/td>\n0.5 mg\/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl and 20% glycerol.<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n38.8 kDa (352 aa, 1-332 aa + His-Tag)<\/td>\n<\/tr>\n
Purity by SDS-PAGE<\/strong><\/td>\n\u226595%<\/td>\n<\/tr>\n
Biological Activity<\/strong><\/td>\nSpecific activity is >20 units\/mg.<\/td>\n<\/tr>\n
Amino Acid Sequence<\/strong><\/td>\nMGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LK<\/td>\n<\/tr>\n
Handling<\/strong><\/td>\nCentrifuge the vial prior to opening.<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737bLi, Hongde et al. (2016) Drosophila larvae synthesize the putative oncometabolite L-2-hydroxyglutarate during normal developmental growth,\u00a0Proc Natl Acad Sci U S A. 2017 Feb 7;114(6):1353-1358.<\/a><\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0Finnpipette F3 \u5fae\u91cf\u5206\u6ce8\u5668<\/a>\u00a0|\u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668<\/strong> Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0Genereach\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/a>\u00a0 |\u00a0\u00a0<\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n

\u00a0<\/strong><\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

LDHA-\u4eba\u985e\u91cd\u7d44\u86cb\u767d Recombinant Protein | \u8ca8\u865f6374-100 Catalyzes […]<\/p>\n","protected":false},"author":3,"featured_media":21580,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[461],"tags":[1992],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21579"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21579"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21579\/revisions"}],"predecessor-version":[{"id":21581,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21579\/revisions\/21581"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/21580"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21579"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21579"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21579"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}