{"id":21528,"date":"2019-07-29T20:14:24","date_gmt":"2019-07-29T12:14:24","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21528"},"modified":"2022-07-20T11:02:35","modified_gmt":"2022-07-20T03:02:35","slug":"%e8%9e%a2%e5%85%89%e7%b4%a0%e9%85%b6%e5%a0%b1%e5%b0%8e%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-%e8%b2%a8%e8%99%9fk969-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=21528","title":{"rendered":"\u87a2\u5149\u7d20\u9176\u5831\u5c0e\u5206\u6790\u5957\u7d44 | \u8ca8\u865fK801-200"},"content":{"rendered":"

\u87a2\u5149\u7d20\u9176\u5831\u5c0e\u5206\u6790\u5957\u7d44 | <\/strong>\u8ca8\u865f<\/strong>K801-200<\/a><\/span><\/p>\n

BioVision\u2019s Luciferase Reporter Assay Kit, K801<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong> \u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176 (Firefly luciferase) \u70ba\u5177\u9ad8\u9748\u654f\u5ea6\u4e4b\u5831\u544a\u57fa\u56e0\uff0c\u88ab\u7528\u4f5c\u7814\u7a76\u57fa\u56e0\u8abf\u63a7\u548c\u529f\u80fd\u7814\u7a76\u3002\u8207\u6a19\u6e96\u6c2f\u9ef4\u7d20\u4e59\u9170\u8f49\u79fb\u9176\uff08chloramphenicol acetyltransferase , CAT\uff09\u6e2c\u5b9a\u76f8\u6bd4\uff0c\u87a2\u5149\u7d20\u9176 (Firefly luciferase)\u51771000\u500d\u7684\u9748\u654f\u5ea6\u3002\u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176(Firefly luciferase)\u50ac\u5316\u87a2\u5149\u7d20(luciferin)\u7684\u6c27\u5316\u7fa7\u5316(oxidative carboxylation)\uff0c\u662f\u5df2\u77e5\u751f\u7269\u767c\u5149\u53cd\u61c9\u7684\u6700\u9ad8\u6548\u7387\u7684\u53cd\u61c9\u3002\u51b7\u5149\u53cd\u61c9\u53ef\u4ee5\u4f7f\u7528luminometer\u51b7\u5149\u5100\u5668\u6e2c\u91cf\u53cd\u61c9\u7684light emission\u3002 BioVision\u7684\u87a2\u5149\u7d20\u9176\u5831\u5c0e\u5206\u6790\u8a66\u5291\u76d2 (BioVision\u2019s Luciferase Reporter Assay Kit, K801) \u63d0\u4f9b\u4e86\u4e00\u7a2e\u6aa2\u6e2c\u8f49\u67d3\u7684\u771f\u6838\u7d30\u80de\u4e2d\u87a2\u5149\u7d20\u9176\u6d3b\u6027\u7684\u7c21\u55ae\u65b9\u6cd5\u3002\u70ba\u4e86\u5728\u9ad4\u5916\u6e2c\u91cf\u8868\u9054\u7684\u87a2\u5149\u7d20\u9176\uff0c\u9996\u5148\u901a\u904e\u7d30\u80de\u88c2\u89e3\u5f9e\u8f49\u67d3\u7684\u7d30\u80de\u4e2d\u63d0\u53d6\u87a2\u5149\u7d20\u9176\u3002\u7136\u5f8c\u5c07CoA\uff0cATP\uff0cMg 2+\u548c\u7de9\u885d\u6db2\u52a0\u5165\u88c2\u89e3\u7269\u4e2d\uff08\u5305\u542bCoA\u7522\u751f\u5e7e\u4e4e\u6046\u5b9a\u7684\u5149\u767c\u5c04\uff0c\u800c\u4e0d\u662f\u5178\u578b\u7684\u9583\u5149\u52d5\u529b\u5b78\uff09\u3002\u7136\u5f8c\u901a\u904e\u6ce8\u5c04\u87a2\u5149\u7d20\u5f15\u767c\u767c\u5149\u53cd\u61c9\uff0c\u4e26\u901a\u904e\u767c\u5149\u8a08\u8a18\u9304\u767c\u5c04\u7684\u5149\u3002\u901a\u904e\u5728\u512a\u5316\u7684\u7de9\u885d\u6eb6\u6db2\u4e2d\u63d0\u4f9bCoA\uff0c\u4ee5\u53caATP\uff0cMg\u00b2\uff0cBioVision\u7684\u87a2\u5149\u7d20\u9176\u5831\u5c0e\u5206\u6790\u8a66\u5291\u76d2\u53ef\u78ba\u4fdd\u6700\u5927\u9748\u654f\u5ea6\uff0c\u4e00\u81f4\u7684\u5149\u8f38\u51fa\uff0c\u4ee5\u53ca\u4f7f\u7528\u591a\u500b\u6a23\u54c1\u6642\u7684\u65b9\u4fbf\u6027\u548c\u4e00\u81f4\u6027\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/th>\nK801-200<\/td>\n<\/tr>\n
Size<\/th>\n200 assays<\/td>\n<\/tr>\n
Detection Method<\/th>\nLuminescence<\/td>\n<\/tr>\n
Species Reactivity<\/th>\nMammalian<\/td>\n<\/tr>\n
Applications<\/th>\nThis Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells.<\/td>\n<\/tr>\n
Features & Benefits<\/th>\n\u2022 The assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple samples.<\/td>\n<\/tr>\n
Kit Components<\/th>\n\u2022 Substrate A
\n\u2022 Substrate B
\n\u2022 Cell Lysis Buffer<\/td>\n<\/tr>\n
Storage Conditions<\/th>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/th>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/th>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Li, J. et al. (2017) CDC5L Promotes hTERT Expression and Colorectal Tumor Growth, Cell Physiol Biochem. 2017;41(6):2475-2488.<\/p>\n

Jonsson et al., Support of a bi-faceted role of estrogen receptor \u03b2 (ER\u03b2) in ER\u03b1-positive breast cancer cells. Endocr. Relat. Cancer, Jan 2014; 21: 143 – 160.<\/p>\n

Martha Lappas. NOD1 and NOD2 Regulate Proinflammatory and Prolabor Mediators in Human Fetal Membranes and Myometrium via Nuclear Factor-Kappa B. Biol Reprod, Jul 2013; 89: 14.<\/p>\n

Selvaraj Muthusamy et al., Estrogen receptor \u03b2 and 17\u03b2-hydroxysteroid dehydrogenase type 6, a growth regulatory pathway that is lost in prostate cancer, PNAS, Dec 2011; 108: 20090 – 20094.<\/p>\n

Veeraraghavan, J. et. al. Radiation-triggered Tumor Necrosis Factor (TNF) \u03b1-NF{kappa}B Cross-signaling Favors Survival Advantage in Human Neuroblastoma Cells. J. Biol. Chem., Jun 2011; 286: 21588 – 21600.<\/p>\n

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\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

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