{"id":21407,"date":"2019-07-19T16:30:55","date_gmt":"2019-07-19T08:30:55","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21407"},"modified":"2022-07-20T11:06:49","modified_gmt":"2022-07-20T03:06:49","slug":"xod%e9%bb%83%e5%98%8c%e5%91%a4%e6%b0%a7%e5%8c%96%e9%85%b6%e6%b4%bb%e6%80%a7%e6%af%94%e8%89%b2%e8%9e%a2%e5%85%89%e6%aa%a2%e6%b8%ac-%e8%b2%a8%e8%99%9fk710-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=21407","title":{"rendered":"XOD\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6d3b\u6027\u6bd4\u8272\/\u87a2\u5149\u6aa2\u6e2c | \u8ca8\u865fK710-100"},"content":{"rendered":"

\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6d3b\u6027\u6bd4\u8272\/\u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K710-100<\/a><\/span><\/p>\n

Xanthine Oxidase Activity Colorimetric\/Fluorometric Assay Kit<\/em><\/strong>\u00a0<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u9ec3\u560c\u5464\u6c27\u5316\u9176\uff08Xanthine oxidase , XO\uff0cEC 1.17.3.2\uff09\u5728\u5065\u5eb7\u500b\u9ad4\u7684\u809d\u81df(liver)\u548c\u7a7a\u8178(jejunum)\u4e2d\u4ee5\u53ef\u89c0\u91cf\u5b58\u5728\u3002 \u7136\u800c\uff0c\u5728\u5404\u7a2e\u809d\u81df\u75be\u75c5(liver disorders)\u4e2d\uff0cXO\u88ab\u91cb\u653e\u5230\u5faa\u74b0\u4e2d\u3002 \u56e0\u6b64\uff0c\u8840\u6e05XO\u6c34\u5e73\u7684\u6e2c\u5b9a\u53ef\u4f5c\u70ba\u6025\u6027\u809d\u640d\u50b7\u7684\u654f\u611f\u6307\u6a19(acute liver damage)\uff0c\u4f8b\u5982\u9ec3\u75b8(jaundice)\u3002 BioVision\u958b\u767c\u4e86\u4e00\u7a2e\u7c21\u4fbf\u9748\u654f\u7684\u5206\u6790\u65b9\u6cd5\uff0c\u7528\u65bc\u6e2c\u5b9a\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684XO\u9ec3\u560c\u5464\u6c27\u5316\u9176\u3002 \u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cXO\u9ec3\u560c\u5464\u6c27\u5316\u9176\u5c07\u9ec3\u560c\u5464\u6c27\u5316\u6210\u904e\u6c27\u5316\u6c2b\uff08H 2 O 2\uff09\uff0c\u5176\u8207OxiRed TM<\/sup>\u63a2\u91dd\u6309\u5316\u5b78\u8a08\u91cf\u53cd\u61c9\u4ee5\u7522\u751f\u984f\u8272\uff08\u5728\u03bb= 570nm\u8655\uff09\u548c\u87a2\u5149\uff08\u5728Ex \/ Em = 535 \/ 587nm\u8655\uff09\u3002 \u7531\u65bc\u984f\u8272\u6216\u87a2\u5149\u5f37\u5ea6\u8207XO\u542b\u91cf\u6210\u6bd4\u4f8b\uff0c\u56e0\u6b64\u53ef\u4ee5\u7cbe\u78ba\u5730\u6e2c\u91cfXO\u6d3b\u6027\u3002 \u8a72\u8a66\u5291\u76d2\u5728100\u03bcl\u53cd\u61c9\u9ad4\u7a4d\u4e2d\u6aa2\u6e2c\u52301-100 mU\u9ec3\u560c\u5464\u6c27\u5316\u9176\u3002<\/p>\n

\"\"<\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

\n
Ha, Y. et. al. Sigma Receptor 1 Modulates Endoplasmic Reticulum Stress in Retinal Neurons.\u00a0Invest. Ophthalmol. Vis. Sci., Jan 2011; 52: 527 – 540.<\/a><\/div>\n<\/div>\n
\n
Tual-Chalot, S. et al. Circulating Microparticles from Pulmonary Hypertensive rats Induce Endothelial Dysfunction.\u00a0Am. J. Respir. Crit. Care Med.<\/em>, 2010; 182: 261-268.<\/div>\n<\/div>\n
\n
Tual-Chalot, S. et al. Circulating Microparticles from Pulmonary Hypertensive Rats Induce Endothelial Dysfunction. Am. J. Respir. Crit. Care Med. 2010 10.1164\/rccm.200909-1347OC<\/div>\n<\/div>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0Finnpipette F3 \u5fae\u91cf\u5206\u6ce8\u5668<\/a>\u00a0|\u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668<\/strong> Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0Genereach\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/a>\u00a0 |\u00a0\u00a0<\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK710-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (570 nm) or Fluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe kit detects 1-100 mU xanthine oxidase in 100 \u00b5l reaction volume.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ less than 40 minutes
\n\u2022 Fast and convenient<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 XO Assay Buffer
\n\u2022 OxiRed\u2122 Probe
\n\u2022 DMSO (anhydrous)
\n\u2022 XO Enzyme Mix
\n\u2022 XO Substrate Mix
\n\u2022 XO Positive Control (~200mU)
\n\u2022 H\u2082O\u2082 Standard (0.88 M)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6d3b\u6027\u6bd4\u8272\/\u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fK710-100 Xanthine Oxidase Activi […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2047],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21407"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21407"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21407\/revisions"}],"predecessor-version":[{"id":21409,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21407\/revisions\/21409"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21407"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21407"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21407"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}