{"id":21328,"date":"2019-07-15T16:18:22","date_gmt":"2019-07-15T08:18:22","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21328"},"modified":"2022-07-20T11:13:09","modified_gmt":"2022-07-20T03:13:09","slug":"active-leukemia-inhibitory-factor-lif%e6%b4%bb%e6%80%a7%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-%e8%b2%a8%e8%99%9fapa085mu01","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=21328","title":{"rendered":"LIF\u6d3b\u6027\u91cd\u7d44\u86cb\u767d |Active Leukemia Inhibitory Factor\u8ca8\u865fAPA085Mu01"},"content":{"rendered":"

\u9644<\/em><\/strong>Protein marker <\/em><\/strong>\u6216<\/em><\/strong> positive control <\/em><\/strong><\/p>\n

Active Leukemia Inhibitory Factor (LIF)<\/strong>\u6d3b\u6027<\/strong>\u91cd\u7d44\u86cb\u767d <\/strong>|<\/strong>\u8ca8\u865f<\/strong>APA085Mu01<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Cloud clone\u63d0\u4f9b\u539f\u6838\u53ca\u771f\u6838\u751f\u7269\u8868\u73fe\u4e4b\u91cd\u7d44\u86cb\u767d\u7cfb\u5217\u5546\u54c1\uff1b\u5176\u4e2dActive Leukemia Inhibitory Factor (LIF)\u6d3b\u6027\u91cd\u7d44\u86cb\u767d\uff0c\u70ba\u6d3b\u6027\u86cb\u767d\uff0cOrganism Species <\/strong>Mus musculus (Mouse)\u3002Isoelectric Point 9.1\uff0c\u7d14\u5ea6> 95%\uff0c\u63d0\u4f9b\u51cd\u4e7e\u578b\u5f0f\u5305\u88dd\u3002\u53ef\u61c9\u7528\u65bcCell culture; Activity Assays\u7b49\u4e0d\u540c\u7814\u7a76\u3002<\/p>\n

\"APA085Mu01.png\"<\/p>\n

\u86cb\u767d\u8cea\u4fdd\u5b58\u578b\u5f0f<\/strong><\/p>\n

\u86cb\u767d\u8cea\u6eb6PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300\u3002\u70ba\u51b7\u51cd\u4e7e\u71e5\u5305\u88dd\u3002\u4f7f\u7528\u524d10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg\/mL\u907f\u514dvortex. \u77ed\u671f\u4fdd\u5b58Store at 2-8\u00b0C for one month.\u5206\u88dd-80\u00b0C\u9577\u671f12 \u500b\u6708\u3002\u8acb\u907f\u514d\u53cd\u8986\u51b7\u51cd\u89e3\u51cd\u3002<\/p>\n

\"Active<\/p>\n

LIF\u6d3b\u6027\u6e2c\u8a66<\/p>\n

\u767d\u8840\u75c5\u6291\u5236\u56e0\u5b50\uff08Leukemia inhibitory factor , LIF\uff09\u662f\u767d\u7d30\u80de\u4ecb\u7d206\u985e\u7d30\u80de\u56e0\u5b50(interleukin 6 class cytokine)\uff0c\u5176\u901a\u904e\u6291\u5236\u5206\u5316(inhibiting differentiation)\u4f86\u5f71\u97ff\u7d30\u80de\u751f\u9577\u3002 LIF\u4f5c\u70ba\u7d30\u80de\u56e0\u5b50\u9084\u5177\u6709\u53e6\u5916\u7684\u529f\u80fd\uff0c\u5305\u62ec\uff1a\u4e0d\u540c\u985e\u578b\u9776\u7d30\u80de\u7684\u751f\u9577\u4fc3\u9032\u548c\u7d30\u80de\u5206\u5316\uff0c\u5c0d\u9aa8\u4ee3\u8b1d(bone metabolism)\uff0c\u60e1\u75c5\u8cea(cachexia)\uff0c\u795e\u7d93\u767c\u80b2(neural development)\uff0c\u80da\u80ce\u5f62\u6210(embryogenesis)\u548c\u708e\u75c7(inflammation)\u7684\u5f71\u97ff\u3002\u6b64\u5916\uff0c\u83cc\u843d\u523a\u6fc0\u56e0\u5b50\u53d7\u9ad4\uff0c\u7c92\u7d30\u80de\uff08Colony Stimulating Factor Receptor, Granulocyte, \u00a0GCSFR\uff09\u5df2\u88ab\u9451\u5b9a\u70baLIF\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u56e0\u6b64\u9032\u884c\u7d50\u5408ELISA\u6e2c\u5b9a\u4ee5\u6aa2\u6e2c\u91cd\u7d44\u5c0f\u9f20LIF\u548c\u91cd\u7d44\u5c0f\u9f20GCSFR\u7684\u76f8\u4e92\u4f5c\u7528\u3002<\/p>\n

\"\"<\/p>\n

\u5c07LIF\u5728\u542b\u67090.01\uff05BSA\uff08pH7.4\uff09\u7684PBS\u4e2d\u9023\u7e8c\u7a00\u91cb\u3002\u7136\u5f8c\u5c07100\u03bcL\u7684\u91cd\u8907\u6a23\u54c1\u8f49\u79fb\u5230GCSFR\u5305\u88ab\u7684\u5fae\u91cf\u6ef4\u5b9a\u5b54\u4e2d\uff0c\u4e26\u572837\u2103\u4e0b\u5b75\u80b22\u5c0f\u6642\u3002\u7528PBST\u6d17\u6ecc\u5b54\u4e26\u7528\u6297LIF pAb\u5b75\u80b21\u5c0f\u6642\uff0c\u7136\u5f8c\u5438\u51fa\u4e26\u6d17\u6ecc3\u6b21\u3002\u8207HRP\u6a19\u8a18\u7684\u7b2c\u4e8c\u6297\u9ad4\u6eab\u80b2\u5f8c\uff0c\u5438\u51fa\u5b54\u4e26\u6d17\u6ecc3\u6b21\u3002\u52a0\u5165\u5e95\u7269\u6eb6\u6db2\uff0c\u5c07\u5b54\u572837\u2103\u4e0b\u5b75\u80b215-25\u5206\u9418\u3002\u6700\u5f8c\uff0c\u5411\u5b54\u4e2d\u52a0\u516550\u03bcL\u7d42\u6b62\u6eb6\u6db2\uff0c\u7acb\u5373\u8b80\u53d6450nm\u3002 LIF\u548cGCSFR\u7684\u7d50\u5408\u6d3b\u6027\u5982\u57161\u6240\u793a\uff0c\u9019\u7a2e\u4f5c\u7528\u5448\u5291\u91cf\u4f9d\u8cf4\u6027\u3002 LIF\u8207GCSFR\u7684\u7d50\u5408\u6d3b\u6027\u3002<\/p>\n

LIF<\/strong>\u767d\u8840\u75c5\u6291\u5236\u56e0\u5b50<\/strong>\u6d3b\u6027<\/strong>\u91cd\u7d44\u86cb\u767d<\/strong>\u76f8\u95dc\u4e4b\u6297\u9ad4<\/strong> | <\/strong>\u86cb\u767d\u8cea<\/strong> \u7522\u54c1<\/strong><\/p>\n\n\n\n\n\n\n
Catalog No.<\/td>\nRelated products for research use of Mus musculus (Mouse) Organism species<\/td>\nApplications (RESEARCH USE ONLY!)<\/td>\n<\/tr>\n
APA085Mu01<\/td>\nActive Leukemia Inhibitory Factor (LIF)<\/strong><\/a><\/td>\nCell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n
RPA085Mu01<\/td>\nRecombinant Leukemia Inhibitory Factor (LIF)<\/strong><\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
PAA085Mu01<\/td>\nPolyclonal Antibody to Leukemia Inhibitory Factor (LIF)<\/strong><\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u9644Protein marker \u6216 positive control Active Leukemia Inhi […]<\/p>\n","protected":false},"author":3,"featured_media":16383,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[461],"tags":[2073],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21328"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21328"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21328\/revisions"}],"predecessor-version":[{"id":21331,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21328\/revisions\/21331"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16383"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21328"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21328"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21328"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}