{"id":21001,"date":"2019-06-17T16:27:35","date_gmt":"2019-06-17T08:27:35","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=21001"},"modified":"2022-07-20T11:34:24","modified_gmt":"2022-07-20T03:34:24","slug":"ponceau-s-%e9%ba%97%e6%98%a5%e7%b4%85-%e8%9b%8b%e7%99%bd%e8%b3%aa%e6%9f%93%e5%8a%91-ponceau-s-solution-expedeon%e8%b2%a8%e8%99%9f5999-1l","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=21001","title":{"rendered":"Ponceau S  (\u9e97\u6625\u7d05) \u86cb\u767d\u8cea\u67d3\u5291 | Ponceau S Solution Expedeon\u8ca8\u865f5999-1L\/ab270042"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=21001\">Ponceau S\u86cb\u767d\u8cea\u67d3\u5291 |\u8ca8\u865f5999-1L<\/a>\/ab270042<\/span><\/p>\n<p>\u9e97\u6625\u7d05Ponceau S-blotting membranes\u6548\u7387\u53ef\u8996\u5316\u67d3\u5291<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.expedeon.com\/wp-content\/uploads\/2018\/07\/Ponceau-S-20118.jpg\" alt=\"\" width=\"307\" height=\"392\" \/><\/p>\n<p><strong>\u7522\u54c1\u654d\u8ff0: <\/strong>Ponceau S\u6eb6\u6db2\u5373\u7528\u578b\u53ef\u9006\u67d3\u8272\u5291\u3002\u5feb\u901f\u6aa2\u6e2c\u785d\u9178\u7e96\u7dad\u7d20(nitrocellulose)\u3001PVDF\u819c\u3001cellulose acetate membranes\u4e0a\u86cb\u767d\u8cea\u7684\u67d3\u8272\u8a66\u5291\u3002\u64cd\u4f5c\u7c21\u55ae\uff0c\u5728\u4f7f\u7528Ponceau S\u6eb6\u6db2\u53cd\u61c95\u5206\u9418\u5167\u9054\u5230\u6700\u9ad8\u67d3\u8272\u5f37\u5ea6\uff0c\u5373\u53ef\u5075\u6e2c\u8f49\u6f2c\u819c(blotting membrane)\u4e0a\u7684\u86cb\u767d\u689d\u5e36 (\u7d05\u8272\u689d\u5e36)\uff0c<strong>\u00a0<\/strong>Ponceau S\u6eb6\u6db2\u53ef\u78ba\u8a8d\u86cb\u767d\u8cea\u7d93\u904e\u8f49\u6f2c\u5f8c\u7684\u72c0\u6cc1\u3002\u86cb\u767d\u8cea\u67d3\u5291\u4e4b\u67d3\u8272\uff0c\u53ef\u65bc\u4e00\u6297\u96dc\u4ea4\u524d\u9032\u884c\uff0c\u78ba\u8a8d\u86cb\u767d\u81a0\u9ad4\u662f\u5426\u5b8c\u5168\u8f49\u6f2c\u6210\u529f\uff0c\u6216\u8f49\u6f2c\u5931\u6557\uff1b\u5373\u53ef\u4e0d\u7528\u6d6a\u8cbb\u9032\u884c\u897f\u65b9\u58a8\u9ede\u6cd5\u5206\u6790\u6642\u6240\u9700\u7684\u8a66\u5291\u8207\u6297\u9ad4\u3002Ponceau S\u6eb6\u6db2\u539f\u7406\u70ba\u8ca0\u67d3\u8272(Negative stain)\u8207\u86cb\u767d\u8cea\u7684\u5e36\u6b63\u96fb(positively charged)\u80fa\u57fa(amino groups)\u7d50\u5408\uff1b\u9084\u975e\u5171\u50f9\u7d50\u5408\u86cb\u767d\u8cea\u4e2d\u7684\u975e\u6975\u6027\u5340\u57df\u3002\u67d3\u8272\u53cd\u61c9\u70ba\u53ef\u9006\u6027(reversible)\uff0c\u86cb\u767d\u8cea\u53ef\u4ee5\u9032\u4e00\u6b65\u9032\u884c\u514d\u75ab\u5b78\u6aa2\u6e2c( immunological detection)\u3002 Ponceau S\u5141\u8a31\u7528\u6236\u5728Western blotting\u904e\u7a0b\u4e2d\uff0cmembrane blocking \u53ca protein detection\u524d\uff0c\u78ba\u8a8d\u86cb\u767d\u8cea\u8f49\u79fb\u81f3blotting membranes\u7684\u6548\u7387\u3002\u6b64\u7522\u54c1\u8207\u5927\u90e8\u4efdblocking agents\u5177\u76f8\u5bb9\u6027\uff1b\u4f8b\u5982 non-fat dry milk (3-5%)\u3001casein (1%) \u53cafish skin gelatin (1%)\u3002\u9069\u7528\u65bcNC\u819c\u3001PVDF\u819c\u53cacellulose acetate membranes\uff1b\u4f46\u4e0d\u5efa\u8b70\u4f7f\u7528\u65bcnylon membranes\u3002\u5728\u9084\u539f\u548c\u975e\u9084\u539f\u689d\u4ef6\u4e0b\u90fd\u5177\u6709\u76f8\u540c\u7684\u6aa2\u6e2c\u9748\u654f\u5ea6\u548c\u518d\u73fe\u6027\u3002Ponceau S\u6eb6\u6db2\u53ef\u4ee5\u5728\u785d\u9178\u7e96\u7dad\u7d20\u819c\u4e0a\u67d3\u8272\u5c11\u81f3250ng\u7684\u725b\u8840\u6e05\u767d\u86cb\u767d\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.expedeon.com\/wp-content\/uploads\/2018\/07\/New-Ponceau-S-Image--686x1024.jpg\" alt=\"Staining reagent for the detection of proteins on nitrocellulose and PVDF membranes.\" width=\"222\" height=\"308\" \/><\/p>\n<p>Figure 1: Runblue 4-12% Bis-Tris gel. Lanes 1,8 Thermo Scientific Page Ruler Plus, lane 2 E.coli lysate, lane 3 Chicken lysate, lane 4 2ug BSA, lane 5 1ug BSA, lane 6 500ng BSA, lane 7 250ng BSA<\/p>\n<p>\u819c\u8f49\u79fb\u5f8c\uff0c\u5c07blotting membrane\u76f4\u63a5\u5728~15ml\u7684Ponceau S\u6eb6\u6db2\u4e2d\u53cd\u61c9\uff08PVDF\u6216\u785d\u9178\u7e96\u7dad\u7d20\u819c\uff09\uff0c\u7121\u9700\u4efb\u4f55\u6d17\u6ecc\u6b65\u9a5f\u3002 \u6240\u6709\u8f49\u79fb\u7684\u86cb\u767d\u8cea\u5728\u819c\u4e0a\u4ee5\u7d05\u8272\u689d\u5e36\u5448\u73fe\uff0c\u5728\u8207Ponceau S\u5b75\u80b2\u76845\u5206\u9418\u5167\u9054\u5230\u6700\u9ad8\u67d3\u8272\u5f37\u5ea6\u3002\u5728Ponceau S\u67d3\u8272\u5f8c\u7528\u6c34\u6c96\u6d17\u819c\u53ef\u6709\u52a9\u65bc\u6e1b\u5c11\u5370\u8de1\u819c\u4e0a\u7684\u201c\u80cc\u666f\u201d\u67d3\u8272\u3002 \u67d3\u8272\u5f37\u5ea6\u5c0d\u61c9\u65bc\u8f49\u79fb\u7684\u86cb\u767d\u8cea\u7684\u91cf\u3002Note: Ponceau S is not suitable for use with nylon membranes<\/p>\n<p>\u71b1\u9580\u5546\u54c1 <span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7539\">400nm\u4e73\u81a0\u73e0\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 (\u7d05\u8272)<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7548\">400nm\u4e73\u81a0\u73e0\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 (\u85cd\u8272) <\/a>| <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7556\">400nm\u4e73\u81a0\u73e0\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44 (\u9ed1\u8272)<\/a>\u76f8\u95dc\u5546\u54c1 <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6535\">Streptavidin-gold\u5948\u7c73\u91d1\u7c92\u5b50(40nm)<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6538\">Streptavidin-gold\u5948\u7c73\u91d1\u7c92\u5b50(20nm)<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6528\">Biotin-gold\u5948\u7c73\u91d1\u7c92\u5b50(40nm)<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=2603\">10-80nm\u5948\u7c73\u91d1\u6297\u9ad4\u6a19\u8a8c\u5957\u7d44<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=3092\">\u5948\u7c73\u91d1\u6eb6\u6db2<\/a><\/span><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ponceau S\u86cb\u767d\u8cea\u67d3\u5291 |\u8ca8\u865f5999-1L\/ab270042 \u9e97\u6625\u7d05Ponceau S-blottin [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":21002,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[97],"tags":[2119],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21001"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21001"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21001\/revisions"}],"predecessor-version":[{"id":24538,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/21001\/revisions\/24538"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/21002"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21001"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21001"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21001"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}