{"id":20588,"date":"2019-06-03T16:36:08","date_gmt":"2019-06-03T08:36:08","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=20588"},"modified":"2022-07-20T11:44:20","modified_gmt":"2022-07-20T03:44:20","slug":"active-fibroblast-growth-factor-2-basic-fgf2%e6%b4%bb%e6%80%a7%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-%e8%b2%a8%e8%99%9fapa551hu01","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=20588","title":{"rendered":"FGF2\u6d3b\u6027\u91cd\u7d44\u86cb\u767d |Active Fibroblast Growth Factor 2, Basic\u8ca8\u865fAPA551Hu01"},"content":{"rendered":"

\u9644<\/em><\/strong>Protein marker <\/em><\/strong>\u6216<\/em><\/strong> positive control <\/em><\/strong><\/p>\n

Active Fibroblast Growth Factor 2, Basic (FGF2)<\/strong>\u6d3b\u6027<\/strong>\u91cd\u7d44\u86cb\u767d <\/strong>|<\/strong>\u8ca8\u865f<\/strong>APA551Hu01<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Cloud clone\u63d0\u4f9b\u539f\u6838\u53ca\u771f\u6838\u751f\u7269\u8868\u73fe\u4e4b\u91cd\u7d44\u86cb\u767d\u7cfb\u5217\u5546\u54c1\uff1b\u5176\u4e2dActive Fibroblast Growth Factor 2, Basic (FGF2)\u6d3b\u6027\u91cd\u7d44\u86cb\u767d\uff0c\u70ba\u6d3b\u6027\u86cb\u767d\uff0cOrganism Species <\/strong>Homo sapiens (Human)\u3002Isoelectric Point 9.6\uff0c\u7d14\u5ea6> 95%\uff0c\u63d0\u4f9b\u51cd\u4e7e\u578b\u5f0f\u5305\u88dd\u3002\u53ef\u61c9\u7528\u65bcCell culture; Activity Assays\u7b49\u4e0d\u540c\u7814\u7a76\u3002\u6210\u7e96\u7dad\u7d30\u80de\u751f\u9577\u56e0\u5b50\uff08Basic fibroblast growth factor , FGF2\uff09\uff0c\u4e5f\u7a31\u70babFGF\uff0cFGF-\u03b2\u662f\u53c3\u8207\u7d30\u80de\u589e\u6b96 ( cell proliferation)\uff0c\u751f\u9577(growth)\u548c\u5206\u5316 (differentiation) \u8abf\u7bc0\u7684\u5927\u7d50\u69cb\u76f8\u95dc\u809d\u7d20\u7d50\u5408\u86cb\u767d\uff08FGF\uff09\u5bb6\u65cf\u7684\u6210\u54e1\u3002\u5b83\u6d89\u53ca\u8a31\u591a\u751f\u7269\u904e\u7a0b\uff0c\u5305\u62ec\u8840\u7ba1\u751f\u6210 (angiogenesis)\uff0c\u80da\u80ce\u767c\u80b2 (embryonic development)\u548c\u50b7\u53e3\u7652\u5408(wound healing)\u3002\u53e6\u5916\uff0cFGF2\u662f\u4eba\u80da\u80ce\u5e79\u7d30\u80de (human embryonic stem cell)\u57f9\u990a\u57fa\u7684\u95dc\u9375\u7d44\u5206\u3002\u6b64\u5916\uff0cCaspase 1\uff08CASP1\uff09\u5df2\u88ab\u9451\u5b9a\u70baFGF2\u7684\u76f8\u4e92\u4f5c\u7528\u3002<\/p>\n

\"\"\"\"<\/p>\n

\u86cb\u767d\u8cea\u4fdd\u5b58\u578b\u5f0f<\/strong><\/p>\n

\u86cb\u767d\u8cea\u6eb6\u65bc20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.\u70ba\u51b7\u51cd\u4e7e\u71e5\u5305\u88dd\u3002\u4f7f\u7528\u524dReconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg\/mL.\u907f\u514dvortex. \u77ed\u671f\u4fdd\u5b58Store at 2-8\u00b0C for one month.\u5206\u88dd-80\u00b0C\u9577\u671f12 \u500b\u6708\u3002\u8acb\u907f\u514d\u53cd\u8986\u51b7\u51cd\u89e3\u51cd\u3002<\/p>\n

\"\"<\/p>\n

ELISA\u6e2c\u5b9a\u4ee5\u6aa2\u6e2c\u91cd\u7d44\u4ebaFGF2\u8207\u91cd\u7d44\u4ebaCASP1\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u5c07FGF2\u5728\u542b\u67090.01\uff05BSA\uff08pH7.4\uff09\u7684PBS\u4e2d\u9023\u7e8c\u7a00\u91cb\u3002\u7136\u5f8c\u5c07100uL\u7684\u91cd\u8907\u6a23\u54c1\u8f49\u79fb\u5230CASP1\u5305\u88ab\u7684\u5fae\u91cf\u6ef4\u5b9a\u5b54\u4e2d\uff0c\u4e26\u572837\u2103\u4e0b\u5b75\u80b22\u5c0f\u6642\u3002\u7528PBST\u6d17\u6ecc\u5b54\u4e26\u8207\u6297FGF2 pAb\u4e00\u8d77\u6eab\u80b21\u5c0f\u6642\uff0c\u7136\u5f8c\u5438\u51fa\u4e26\u6d17\u6ecc3\u6b21\u3002\u8207HRP\u6a19\u8a18\u7684\u7b2c\u4e8c\u6297\u9ad4\u6eab\u80b2\u5f8c\uff0c\u5438\u51fa\u5b54\u4e26\u6d17\u6ecc3\u6b21\u3002\u52a0\u5165\u5e95\u7269\u6eb6\u6db2\uff0c\u5c07\u5b54\u572837\u2103\u4e0b\u5b75\u80b215-25\u5206\u9418\u3002\u6700\u5f8c\uff0c\u5411\u5b54\u4e2d\u52a0\u516550\u03bcL\u7d42\u6b62\u6eb6\u6db2\uff0c\u7acb\u5373\u8b80\u53d6450nm\u3002 FGF2\u548cCASP1\u7684\u7d50\u5408\u6d3b\u6027\u986f\u793a\u5728\u57161\u4e2d\uff0c\u4e26\u4e14\u8a72\u4f5c\u7528\u662f\u5291\u91cf\u4f9d\u8cf4\u6027\u7684<\/p>\n

FGF2<\/strong>\u6d3b\u6027<\/strong>\u91cd\u7d44\u86cb\u767d<\/strong>\u76f8\u95dc\u4e4b\u6297\u9ad4<\/strong> | <\/strong>\u86cb\u767d\u8cea<\/strong> \u7522\u54c1<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Catalog No.<\/td>\nRelated products for research use of Homo sapiens (Human) Organism species<\/td>\nApplications (RESEARCH USE ONLY!)<\/td>\n<\/tr>\n
RPA551Hu01<\/td>\nRecombinant Fibroblast Growth Factor 2, Basic (FGF2)<\/strong><\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
APA551Hu01<\/td>\nActive Fibroblast Growth Factor 2, Basic (FGF2)<\/strong><\/a><\/td>\nCell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n
CPA551Hu21<\/td>\nOVA Conjugated Fibroblast Growth Factor 2, Basic (FGF2)<\/strong><\/a><\/td>\nImmunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
PAA551Hu01<\/td>\nPolyclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2)<\/strong><\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
LAA551Hu71<\/td>\nBiotin-Linked Polyclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2)<\/strong><\/a><\/td>\nWB; IHC; ICC.<\/td>\n<\/tr>\n
LAA551Hu81<\/td>\nFITC-Linked Polyclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2)<\/strong><\/a><\/td>\nWB; IHC; ICC; IF.<\/td>\n<\/tr>\n
MAA551Hu22<\/td>\nMonoclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2)<\/strong><\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
SEA551Hu<\/td>\nELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)<\/strong><\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
CEA551Hu<\/td>\nELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)<\/strong><\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
KSA551Hu11<\/td>\nELISA Kit DIY Materials for Fibroblast Growth Factor 2, Basic (FGF2)<\/strong><\/a><\/td>\nMain materials for “Do It (ELISA Kit) Yourself”.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a06\u516c\u5347\u8ff7\u4f60\u57f9\u990a\u7bb1<\/a>\u00a0|\u00a0<\/strong>\u6ec5\u83cc\u91dc<\/strong><\/a>\u00a0 |\u00a0<\/strong>\u6c34\u6d74\u69fd<\/strong>WaterBath<\/strong><\/em><\/a>\u00a0|\u00a0<\/strong>\u96fb\u5b50\u5929\u5e73<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>pH<\/strong>\u9178\u9e7c\u5ea6\u8a08<\/strong><\/a>\u00a0|\u00a0<\/strong>\u00a0Vortex\u00a0<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>Pipet aid\u00a0<\/a>\u00a0|\u00a0ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/strong><\/strong><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u9644Protein marker \u6216 positive control Active Fibroblast Gr […]<\/p>\n","protected":false},"author":3,"featured_media":16383,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[461],"tags":[2152,2151],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/20588"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=20588"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/20588\/revisions"}],"predecessor-version":[{"id":20590,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/20588\/revisions\/20590"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16383"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=20588"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=20588"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=20588"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}