{"id":19991,"date":"2019-05-24T16:48:52","date_gmt":"2019-05-24T08:48:52","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19991"},"modified":"2022-07-20T11:46:00","modified_gmt":"2022-07-20T03:46:00","slug":"%e8%84%82%e8%b3%aa%e9%81%8e%e6%b0%a7%e5%8c%96%e6%aa%a2%e6%b8%ac-lipid-peroxidation-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk454-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=19991","title":{"rendered":"\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c | Lipid Peroxidation Colorimetric Assay Kit\u8ca8\u865fK454-100"},"content":{"rendered":"

\u8102\u8cea\u904e\u6c27\u5316\u6bd4\u8272\u6e2c\u5b9a | <\/strong>\u8ca8\u865f<\/strong>K454-100<\/a><\/span><\/p>\n

Lipid Peroxidation Colorimetric Assay Kit<\/em><\/strong><\/p>\n

\"\"\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u8102\u8cea\u904e\u6c27\u5316(Lipid peroxidation)\u662f\u4e00\u7a2e\u5f71\u97ff\u7d30\u80de\u819c(cellular membranes) \uff0c\u8102\u86cb\u767d(lipoproteins)\u548c\u5176\u4ed6\u542b\u8102\u8cea\u5206\u5b50(lipid-containing molecules) \u7684\u6c27\u5316\u640d\u50b7(oxidative damage)\u3002\u8a72\u7cfb\u5217\u93c8\u53cd\u61c9(chain reactions)\u767c\u751f\u5728\u9ad8\u6d3b\u6027\u6c27\u7269\u8cea(reactive oxygen species)\u548c\u7d30\u80de\u819c\u4e2d\u7684\u4e0d\u98fd\u548c\u8102\u80aa\u9178(unsaturated fatty acids)\u4e4b\u9593\uff0c\u9019\u5c0e\u81f4\u7d30\u80de\u640d\u50b7(cell damage)\u3002\u6e90\u81ea\u591a\u4e0d\u98fd\u548c\u8102\u80aa\u9178(polyunsaturated fatty acids) \u7684\u8102\u8cea\u904e\u6c27\u5316\u7269(Lipid peroxides)\u662f\u4e0d\u7a69\u5b9a\u7684\u4e26\u4e14\u5206\u89e3\u5f62\u6210\u4e19\u4e8c\u919b\uff08malondialdehyde , MDA\uff09\u548c4-\u7fa5\u57fa\u70ef\u919b(4-hydroxyalkenals) \u3002 MDA\u7684\u6e2c\u91cf\u88ab\u5ee3\u6cdb\u4f7f\u7528\u4e26\u4e14\u88ab\u8a8d\u70ba\u662f\u8102\u8cea\u904e\u6c27\u5316(lipid peroxidation) \u7684\u6307\u6a19\u3002\u50b3\u7d71\u4e0a\uff0c2-\u786b\u4ee3\u5df4\u6bd4\u59a5\u9178\uff082-Thiobarbituric acid , TBA\uff09\u65b9\u6cd5\u5df2\u5ee3\u6cdb\u7528\u65bc\u6a23\u54c1\u4e2dMDA\u7684\u6bd4\u8272\u6aa2\u6e2c\u548c\u6e2c\u91cf\u3002\u7136\u800c\uff0c\u8a72\u53cd\u61c9\u662f\u76f8\u5c0d\u975e\u7279\u7570\u6027\u7684\uff0c\u56e0\u70ba\u6e38\u96e2\u7684\u548c\u86cb\u767d\u8cea\u7d50\u5408\u7684MDA\u90fd\u8207TBA\u53cd\u61c9\u3002 BioVision\u7684\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2(Lipid Peroxidation Assay Kit, K454) \u4f7f\u7528\u4e00\u5957\u5c08\u6709\u8a66\u5291\uff0c\u53ef\u6700\u5927\u9650\u5ea6\u5730\u6e1b\u5c11\u5176\u4ed6\u8102\u8cea\u904e\u6c27\u5316\u7522\u7269\uff08\u5305\u62ec4-hydroxyalkenals., 4-\u7fa5\u57fa\u70ef\u919b\uff09\u7684\u5e72\u64fe\u3002\u8a72\u539f\u7406\u57fa\u65bc\u7279\u5b9a\u986f\u8272\u8a66\u5291\u548cMDA\u572845\u2103\u4e0b\u7684\u53cd\u61c9\u3002\u8a72\u6e2c\u5b9a\u6cd5\u6613\u65bc\u5be6\u65bd\uff0c\u4e26\u7522\u751f\u5f37\u800c\u7a69\u5b9a\u7684\u6bd4\u8272\u4fe1\u865f\uff08OD 586nm\uff09\uff0c\u5176\u8207\u6a23\u54c1\u4e2dMDA\u7684\u91cf\u6210\u6bd4\u4f8b\u3002\u6211\u5011\u7684\u5206\u6790\u53ef\u4ee5\u6aa2\u6e2c\u6a23\u54c1\u4e2d\u4f4e\u81f33 nmol\u7684MDA\u3002 MDA +\u986f\u8272\u5291=\u9aee\u8272\u5718\uff08OD 586 nm\uff09\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK454-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\nBioVision\u2019s Lipid Peroxidation Assay Kit uses a proprietary set of reagents that minimizes the interference from other lipid peroxidation products including 4-hydroxyalkenals. The principle is based on the reaction between a specific chromogenic reagent and MDA at 45 \u00b0C. The assay is simple to perform, and yields a strong, yet stable colorimetric signal (OD 586 nm) that is proportional to the amount of MDA in samples. Our assay can detect as low as 3 nmol MDA in samples.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nColorimetric assay (OD 586 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nAll<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasurement of lipid peroxidation in tissues and serum samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Specific
\n\u2022 Simple procedure
\n\u2022 Can detect as low as 3 nmol MDA in samples
\n\u2022 Fast and convenient<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Diluent A
\n\u2022 Chromogenic Reagent
\n\u2022 MDA Standard
\n\u2022 Diluent B
\n\u2022 BHT<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u8102\u8cea\u904e\u6c27\u5316\u6bd4\u8272\u6e2c\u5b9a | \u8ca8\u865fK454-100 Lipid Peroxidation Colorimetric […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[183],"tags":[2167],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19991"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19991"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19991\/revisions"}],"predecessor-version":[{"id":20485,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19991\/revisions\/20485"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19991"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19991"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19991"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}