{"id":19843,"date":"2019-05-17T10:48:06","date_gmt":"2019-05-17T02:48:06","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19843"},"modified":"2022-07-20T13:48:56","modified_gmt":"2022-07-20T05:48:56","slug":"link-fast5%e5%88%86%e9%90%98dna%e9%80%a3%e6%8e%a5%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk902-50","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=19843","title":{"rendered":"Link-FAST\u21225\u5206\u9418DNA\u9023\u63a5\u8a66\u5291\u76d2 | \u8ca8\u865fK902-50"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=19843\">Link-FAST\u21225\u5206\u9418DNA\u9023\u63a5\u8a66\u5291\u76d2 | \u8ca8\u865fK902-50<\/a><\/span><\/p>\n<p><em>Link-FAST\u2122 5 Minutes DNA Ligation Kit<\/em><em>\u00a0<\/em><\/p>\n<p><strong>\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>Link-FAST\u21225\u5206\u9418DNA\u9023\u63a5\u8a66\u5291\u76d2 (5 minutes DNA Ligation Kit, K902) \u53ef\u5728\u5ba4\u6eab\u4e0b\u50c55\u5206\u9418\u5167\u5feb\u901f\u65b9\u4fbf\u5730\u9023\u63a5\u7c98\u7aef(sticky-end)\u6216\u920d\u7aef(blunt-end ) DNA\u7247\u6bb5(DNA fragments)\u3002 \u63d0\u4f9b\u4e86\u9023\u63a5\u6240\u9700\u7684\u6240\u6709\u5fc5\u9700\u8a66\u5291 ( Lipase Assay Buffer\u3001OxiRed Probe\u3001Dimethylsulfoxide (DMSO)\u3001 Enzyme Mix (lyophilized)\u3001Lipase Substrate\u3001 Glycerol Standard (100mM)\u3001 Lipase Positive Control\u3002 \u7121\u9700\u88fd\u5099\u7de9\u885d\u6db2\u6216\u6dfb\u52a0ATP\u6216Mg\u00b2\u1da7\u3002 \u8a72\u8a66\u5291\u76d2\u53ef\u7528\u65bc\u5728\u8cea\u9ad4\u8f09\u9ad4(plasmid vectors)\u6216\u566c\u83cc\u9ad4\u8f09\u9ad4 (phage vectors) r cloning\uff0clinker ligation\uff0c\u7dda\u6027DNA\u7684recirculation\u7b49\u3002\u53ef\u901a\u904e\u74ca\u8102\u7cd6\u51dd\u81a0\u96fb\u6cf3\u5bb9\u6613\u5730\u5206\u6790Ligated DNA\u3002<\/p>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Cat # +Size<\/strong><\/td>\n<td>K902-50<\/td>\n<\/tr>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>50 ligations<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>The 5 minutes DNA Ligation Kit provides unique formulations of reagents for ligation of sticky-end or blunt-end DNA in just 5 minutes.<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>\u2022 All necessary reagents are provided for cloning in plasmid vectors\/phage vectors\/linker ligation\/recirculation of linear DNA and more .<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 DNA Dilution Buffer<br \/>\n\u2022 2X T4 DNA Ligation Buffer<br \/>\n\u2022 T4 DNA Ligase (5 units\/\u00b5l)<\/td>\n<\/tr>\n<tr>\n<td><strong>Storage Conditions<\/strong><\/td>\n<td>-80\u00b0C<\/td>\n<\/tr>\n<tr>\n<td><strong>Shipping Conditions<\/strong><\/td>\n<td>Dry Ice<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u00a0<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Link-FAST\u21225\u5206\u9418DNA\u9023\u63a5\u8a66\u5291\u76d2 | \u8ca8\u865fK902-50 Link-FAST\u2122 5 Minutes  [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[133],"tags":[2195],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19843"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19843"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19843\/revisions"}],"predecessor-version":[{"id":19844,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19843\/revisions\/19844"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19843"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19843"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19843"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}