{"id":19779,"date":"2019-05-09T15:53:02","date_gmt":"2019-05-09T07:53:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19779"},"modified":"2022-07-20T13:50:49","modified_gmt":"2022-07-20T05:50:49","slug":"ldl%e6%94%9d%e5%8f%96%e5%88%86%e6%9e%90%e8%a9%a6%e5%8a%91%e7%9b%92-ldl-uptake-assay-kit-%e8%b2%a8%e8%99%9fk436-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=19779","title":{"rendered":"LDL\u651d\u53d6\u5206\u6790\u8a66\u5291\u76d2 | LDL Uptake Assay Kit \u8ca8\u865fK436-100"},"content":{"rendered":"

LDL<\/strong>\u651d\u53d6\u5206\u6790\u8a66\u5291\u76d2\uff08\u7d30\u80de\uff09 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K436-100<\/a><\/span><\/p>\n

LDL Uptake Assay Kit (Cell-Based)<\/em><\/strong>\u00a0<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\uff08Low-density lipoprotein , LDL\uff09\u662f\u4e00\u7a2e\u5927\u7403\u5f62\u5927\u5206\u5b50\uff0c\u53ef\u4f5c\u70ba\u201cshuttle\u7a7f\u68ad\u201d\uff0c\u901a\u904e\u8840\u6db2\u7684\u6c34\u6027\u74b0\u5883\u5c07hydrophobic molecules\u758f\u6c34\u6027\u5206\u5b50\uff08lipids\u8102\u8cea\u548ccholesterol\u81bd\u56fa\u9187\uff09\u8f38\u9001\u5230\u5168\u8eab\u3002 LDL\u9846\u7c92\u7531\u81bd\u56fa\u9187\u916f (cholesterol esters) \u7684\u758f\u6c34\u6838\u5fc3\uff08\u8207hydroxyl group\u7fa5\u57fa\u9023\u63a5\u7684long-chain fatty acids\u9577\u93c8\u8102\u80aa\u9178\uff09\u548c\u7531\u542b\u6709510kDa\u86cb\u767d\u8cea\u8f09\u8102\u86cb\u767dB-100\uff0c\u5404\u7a2e\u78f7\u8102 (phospholipids) \u548c\u975e\u916f\u5316\u81bd\u56fa\u9187 (non-esterified cholesterol) \u5206\u5b50\u7684\u5169\u89aa\u6bbc\u5305\u570d\u7684\u7518\u6cb9\u4e09\u916f\u7d44\u6210\u3002 LDL\u901a\u904e\u7279\u7570\u6027\u53d7\u9ad4\u4ecb\u5c0e\u7684\u5167\u541e\u4f5c\u7528\u88ab\u7d30\u80de\u5982\u809d\u7d30\u80de(hepatocytes) \uff0c\u5de8\u566c\u7d30\u80de(macrophages)\u548c\u5167\u76ae\u7d30\u80de(endothelial cells)\u651d\u53d6\u3002\u9ad8\u6c34\u5e73\u7684LDL\u8207\u52d5\u8108\u708e\u75c7(arterial inflammation)\u6709\u95dc\uff0c\u5c0e\u81f4\u5fc3\u8840\u7ba1\u75be\u75c5\uff08CVD\uff09\u5982\u52d5\u8108\u7ca5\u6a23\u786c\u5316(arterial inflammation)\uff0c\u7f3a\u8840\u6027\u4e2d\u98a8(ischemic stroke) \u548c\u5fc3\u808c\u6897\u585e(myocardial infarction)\u3002 LDL\u9846\u7c92\u53ef\u4ee5\u901a\u904e\u767c\u708e\u7684\u8840\u7ba1\u5167\u76ae(vascular endothelial) \u7dca\u5bc6\u9023\u63a5\u9077\u79fb\uff0c\u5728\u90a3\u88e1\u5b83\u5011\u88ab\u6c27\u5316\u4e26\u5438\u5f15\u5faa\u74b0\u7684\u55ae\u6838\u7d30\u80de (monocytes) \u3002\u55ae\u6838\u7d30\u80de(Monocytes differentiate)\u5206\u5316\u6210\u6d3b\u5316\u7684\u5de8\u566c\u7d30\u80de(macrophages)\uff0c\u5176\u9032\u4e00\u6b65\u7a4d\u7d2fLDL\u4e26\u8b8a\u6210\u6ce1\u6cab\u7d30\u80de(foam cells)\uff0c\u5c0e\u81f4\u52d5\u8108\u58c1\u589e\u539a\/\u7622\u75d5\u5f62\u6210(arterial wall thickening\/scaring)\u548c\u7e96\u7dad\u6027\u8102\u80aa\u6591\u584a\u7684\u5f62\u6210(fibrous fatty plaques)\u3002\u76ee\u524d\u7684CVD\u9810\u9632\u7642\u6cd5 (CVD preventative therapies) \u5305\u62ec\u7528\u4ed6\u6c40\u985e\u85e5\u7269(statins)\u964d\u4f4eLDL\uff0c\u5176\u901a\u904e\u6291\u5236HMG-CoA\u9084\u539f\u9176\u4f86\u9810\u9632\u5167\u6e90\u6027\u81bd\u56fa\u9187 (endogenous cholesterol) \u7684\u751f\u7269\u5408\u6210\u3002\u7136\u800c\uff0c\u9084\u7814\u7a76\u4e86\u901a\u904e\u589e\u52a0\u809d\u81dfLDL\u518d\u651d\u53d6\u4f86\u6291\u5236\u5de8\u566c\u7d30\u80de\u651d\u53d6LDL\u6216\u589e\u5f37LDL\u6e05\u9664\u7684\u85e5\u7269\u3002 BioVision\u7684LDL\u651d\u53d6\u5206\u6790\u8a66\u5291\u76d2\u53ef\u6e2c\u91cf\u5404\u7a2e\u7d30\u80de\u985e\u578b\u7684\u53d7\u9ad4\u4ecb\u5c0e\u7684LDL\u651d\u53d6\u3002\u8a72\u8a66\u5291\u76d2\u5305\u542bfluorescent human LDL\uff0c\u5177\u660e\u4eae\u9ad8\u5ea6\u5149\u7a69\u5b9a\u7684\u87a2\u5149\u5718\u5171\u50f9\u6a19\u8a18\uff0c\u7576\u88ab\u7d30\u80de\u651d\u53d6\u6642\u53ef\u4ee5\u6aa2\u6e2c\u5230\uff08Ex \/ Em = 488 \/ 523nm\uff09\u3002\u672a\u6a19\u8a18\u7684human LDL\u5305\u542b\u5728\u8a66\u5291\u76d2\u4e2d\uff0c\u7528\u65bc\u9a57\u8b49\u7279\u7570\u6027\u6a19\u8a18\u7684LDL\u651d\u53d6\u3002\u8a72\u8a66\u5291\u76d2\u5305\u542b\u4e00\u6574\u5957\u8db3\u4ee5\u9032\u884c100\u6b21\u53cd\u61c9\u7684\u8a66\u5291\u548c96\u5b54polylysine\u5857\u5c64\u7684\u9ed1\u8272\u7d30\u80de\u57f9\u990a\u677f\uff0c\u5177\u6709\u8584\u7684\u986f\u5fae\u93e1\u7d1a\u900f\u660e\u5e95\u90e8\u4efd\u3002<\/p>\n

\"\"\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK436-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\nEasy-to-follow protocol for the measurement of LDL uptake in mammalian cell lines.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorometric (Ex\/Em= 488\/523 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nAll<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nScreening and characterization of drugs or treatment conditions that inhibit or stimulate LDL uptake,Characterization of LDL uptake in different cell types.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Measure LDL uptake in different cell types
\n\u2022 Non-Radioactive
\n\u2022 Contains fluorescent human LDL
\n\u2022 which is covalently-labeled with a bright
\n\u2022 highly photostable fluorophore
\n\u2022 Screen
\n\u2022 study or characterize stimulators or inhibitors of LDL uptake<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Flurophore-Labeled LDL (5 mg\/ml)
\n\u2022 Unlabeled LDL
\n\u2022 Polylysine-Coated Cell Culture Plate
\n\u2022 Uptake Assay Buffer<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/a><\/strong>\u00a0LDL\u651d\u53d6\u5206\u6790\u8a66\u5291\u76d2\uff08\u7d30\u80de\uff09<\/a>\u00a0| OxLDL\u00a0\u6c27\u5316\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\u6aa2\u6e2c\u00a0<\/a>\u00a0|\u00a0HDL\u9ad8\u5bc6\u5ea6\u8102\u86cb\u767d\/ LDL\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\/\u975e\u5e38\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

LDL\u651d\u53d6\u5206\u6790\u8a66\u5291\u76d2\uff08\u7d30\u80de\uff09 | \u8ca8\u865fK436-100 LDL Uptake Assay Kit (Cell- […]<\/p>\n","protected":false},"author":3,"featured_media":19780,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[124],"tags":[2207],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19779"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19779"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19779\/revisions"}],"predecessor-version":[{"id":19792,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19779\/revisions\/19792"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/19780"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19779"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19779"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19779"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}