{"id":19523,"date":"2019-04-18T16:31:07","date_gmt":"2019-04-18T08:31:07","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19523"},"modified":"2022-07-20T14:01:44","modified_gmt":"2022-07-20T06:01:44","slug":"%e7%b5%84%e7%b9%94%e8%9b%8b%e7%99%bd%e9%85%b6-s%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-biovision%e8%b2%a8%e8%99%9fk144-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=19523","title":{"rendered":"\u7d44\u7e54\u86cb\u767d\u9176-S\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | Biovision\u8ca8\u865fK144-100"},"content":{"rendered":"

\u7d44\u7e54\u86cb\u767d\u9176-S\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K144-100<\/a><\/span><\/p>\n

Cathepsin S Activity Fluorometric Assay Kit<\/em><\/strong>\u00a0<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong> \u7d44\u7e54\u86cb\u767d\u9176 (Cathepsins) \u662f\u6eb6\u9176\u9ad4\u9176 (lysosomal enzymes) \uff0c\u4e5f\u53ef\u7528\u4f5c\u5404\u7a2e\u6bd2\u7406\u5b78\u7814\u7a76(toxicological investigations) \u4e2d\u7684\u654f\u611f\u6a19\u8a18\u7269\u3002 \u7d44\u7e54\u86cb\u767d\u9176-S\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 (Cathepsin-S Activity Assay kit) \u662f\u57fa\u65bc\u87a2\u5149\u7684\u6e2c\u5b9a\uff0c\u5176\u5229\u7528\u7528AFC\uff08amino-4-trifluoromethyl coumarin\u6c28\u57fa-4-\u4e09\u6c1f\u7532\u57fa\u9999\u8c46\u7d20\uff09\u6a19\u8a18\u7684\u512a\u9078\u7d44\u7e54\u86cb\u767d\u9176-S\u5e95\u7269\u5e8f\u5217VVR(cathepsin-S substrate sequence VVR) \u3002 \u7d30\u80de\u88c2\u89e3\u7269(Cell lysates)\u6216\u542b\u6709\u7d44\u7e54\u86cb\u767d\u9176-S\u7684\u5176\u4ed6\u6a23\u54c1\u5c07\u5207\u5272\u5408\u6210\u5e95\u7269Z-VVR-AFC(substrate Z-VVR-AFC) \u4ee5\u91cb\u653e\u6e38\u96e2AFC (free AFC)\u3002 \u4f7f\u7528\u87a2\u5149\u8a08\u6216\u87a2\u5149\u677f\u8b80\u6578\u5668\u53ef\u4ee5\u8f15\u9b06\u91cf\u5316\u91cb\u653e\u7684AFC\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK144-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em 400\/505)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple one-step procedure; takes only 1-2 hours
\n\u2022 Fast and convenient
\n\u2022 Cathepsin-S assay is straightforward, and can be adapted to 96-well plate assays.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 CS Cell Lysis Buffer
\n\u2022 CS Reaction Buffer
\n\u2022 CS Substrate Z-VVR-AFC (10 mM)
\n\u2022 CS Inhibitor (1 mM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u00a0\u5f15\u7528\u6587\u737b<\/strong><\/p>\n

Chanyang Kim, et al., (2018) IGF-1 protects SH-SY5Y cells against MPP+-induced apoptosis via PI3K\/PDK-1\/Akt pathway, Endocrine Connections, Jun. 2018, 29459421<\/p>\n

McComb et al., Cathepsins Limit Macrophage Necroptosis through Cleavage of Rip1 Kinase.J. Immunol., Jun 2014; 192: 5671 – 5678.<\/p>\n

Chang C-J et al (2009) Am. J. Pathol.; 174: 1837 – 1846.<\/p>\n

Taleb, S., et al. (2006) J. Clin. Endocrinol. Metab. 91: 1042-1047.<\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d44\u7e54\u86cb\u767d\u9176-S\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK144-100 Cathepsin S Activity Fluoro […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2248],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19523"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19523"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19523\/revisions"}],"predecessor-version":[{"id":19525,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19523\/revisions\/19525"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19523"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19523"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19523"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}