{"id":19427,"date":"2019-04-09T14:08:24","date_gmt":"2019-04-09T06:08:24","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19427"},"modified":"2022-07-20T14:04:05","modified_gmt":"2022-07-20T06:04:05","slug":"hamster-anti-mouse-cd81-unlb%e6%8a%97%e9%ab%94-tapa-1%e6%8a%97%e9%ab%94%e8%b2%a8%e8%99%9f1825-01","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=19427","title":{"rendered":"Hamster Anti-Mouse CD81-UNLB\u6297\u9ad4 | TAPA-1\u6297\u9ad4\u8ca8\u865f1825-01"},"content":{"rendered":"

Hamster Anti-Mouse CD81-UNLB\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong><\/a><\/span>1825-01\u00a0<\/a><\/span>\u00a0 <\/strong>\u898f\u683c<\/strong>1.0 mg \u00a0(1.0 mg\/mL)<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> CD81\uff0c\u4e5f\u7a31\u70baTAPA-1\uff0c\u662f\u5728\u591a\u7a2e\u7d30\u80de\u985e\u578b\u4e0a\u8868\u9054\u7684\u5b8c\u6574\u819c\u86cb\u767d(integral membrane protein)\uff0c\u4e26\u4e14\u5728\u4eba\u548c\u5c0f\u9f20\u4e4b\u9593\u5177\u6709\u9ad8\u5ea6\u7684\u5e8f\u5217\u540c\u6e90\u6027(sequence homology)\u3002 CD81\u5728\u80f8\u817a\u57fa\u8cea\u7d30\u80de(thymic stromal cells)\u4e0a\u8868\u9054\uff0c\u5176\u5728\u03b3\u03b4+ T\u7d30\u80de\u5411\u5177\u6709\u03b1\u03b2T\u7d30\u80de\u53d7\u9ad4\u7684\u66f4\u6210\u719fT\u7d30\u80de\u7684\u8f49\u8b8a\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002 \u514d\u75ab\u7d44\u7e54\u5316\u5b78\u67d3\u8272(Immunohistochemical staining)\u986f\u793a\u5176\u8868\u9054\u5b9a\u4f4d\u65bc\u80f8\u817a\u7684\u5305\u819c\u4e0b\u5340\u57df\uff0c\u7279\u5225\u662f\u5177\u6709\u4e0d\u540c\u805a\u985e\u6a21\u5f0f\u7684\u7d30\u80de\u3002 \u64da\u63a8\u6e2c\uff0cCD81\u7684\u914d\u9ad4\u662f\u524dT\u7d30\u80de\u53d7\u9ad4(pre-T cell receptor)\uff0c\u5176\u7531TCR\u03b2\u934a(TCR \u03b2 chain)\u548c\u7cd6\u86cb\u767dpT\u03b1(glycoprotein pT\u03b1)\u7d44\u6210\u3002 \u55ae\u514b\u9686\u6297\u9ad42F7\u53ef\u4ee5\u5728\u9ad4\u5916\u963b\u65b7\u80f8\u817a\u7d30\u80de(thymocyte)\u8207CD81\u7684\u76f8\u4e92\u4f5c\u7528\u3002Hamster Anti-Mouse CD81-UNLB \u6297\u9ad4\uff0c\u4e0d\u5177\u6a19\u8a8c\u3002\u91dd\u5c0dMouse CD81\u5177\u7279\u7570\u6027\u3002\u514d\u75ab\u6e90\u70baMouse epithelial cell line PAM212\u3002Isotype Control Hamster IgG-UNLB<\/a>\u3002\u7de9\u885d\u6db2\u914d\u65b9\u5728Borate buffered saline, pH 8.2\u3002<\/p>\n

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Chinese hamster ovary cell line CHO-K1 and BALB\/cAnN mouse B lymphocyte cell line A20 were stained with Hamster Anti-Mouse CD81-APC (SB Cat. No. 1825-11).<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\n2F7<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nHamster (Armenian) IgG3<\/sub><\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\n<\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nMouse epithelial cell line PAM212<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nMouse CD81<\/td>\n<\/tr>\n
Alternalte Name(s)<\/strong><\/td>\nTAPA-1<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nCD81, also known as TAPA-1, is an integral membrane protein expressed on a variety of cell types and has a high degree of sequence homology between human and mouse. CD81 is expressed on thymic stromal cells where it plays an important role in the transition of \u03b3\u03b4+<\/sup>\u00a0T cells to more mature T cells with \u03b1\u03b2 T cell receptors. Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor which is composed of a TCR \u03b2 chain and glycoprotein pT\u03b1. The monoclonal antibody 2F7 can block thymocyte interaction with CD81\u00a0in vitro<\/em>.<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nUNLB (Unconjugated)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nBorate buffered saline, pH 8.2<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n0.5 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

Flow Cytometry \u2013 Quality tested\u00a01,3<\/sup>
\nImmunohistochemistry \u2013Reported in literature\u00a01<\/sup>
\nImmunocytochemistry \u2013 Reported in literature\u00a05<\/sup>
\nImmunoprecipitation \u2013 Reported in literature\u00a01<\/sup>
\nBlocking \u2013 Reported in literature\u00a01-4<\/sup>
\nCostimulation \u2013 Reported in literature\u00a02<\/sup><\/td>\n<\/tr>\n

Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2795398<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

References<\/strong><\/p>\n

    \n
  1. Boismenu R, Rhein M, Fischer WH, Havran WL. A role for CD81 in early T cell development. Science. 1996;271:198-200. (Immunogen, FC, IP, IHC, Block)<\/li>\n
  2. Takeda Y, He P, Tachibana I, Zhou B, Miyado K, Kaneko H, et al. Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice. J Biol Chem. 2008;283:26089-97. (Block, Costim)<\/li>\n
  3. Tachibana I, Hemler ME. Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. J Cell Biol. 1999;146:893-904. (FC, Block)<\/li>\n
  4. Chang Y, Finnemann SC. Tetraspanin CD81 is required for the \u03b1v\u03b25-integrin-dependent particle-binding step of RPE phagocytosis. J Cell Sci. 2007;120:3053-63. (Block)<\/li>\n
  5. \u00a0Keli\u0107 S, Levy S, Suarez C, Weinstein DE. CD81 regulates neuron-induced astrocyte cell-cycle exit. Mol Cell Neurosci. 2001;17:551-60. (ICC)<\/li>\n<\/ol>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Hamster Anti-Mouse CD81-UNLB\u6297\u9ad4 | \u8ca8\u865f1825-01\u00a0\u00a0 \u898f\u683c1.0 mg \u00a0 […]<\/p>\n","protected":false},"author":3,"featured_media":6494,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[2266],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19427"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19427"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19427\/revisions"}],"predecessor-version":[{"id":19428,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19427\/revisions\/19428"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6494"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19427"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19427"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19427"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}