{"id":19411,"date":"2019-04-08T13:39:02","date_gmt":"2019-04-08T05:39:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19411"},"modified":"2022-07-20T14:04:30","modified_gmt":"2022-07-20T06:04:30","slug":"mouse-anti-influenza-a-nucleoprotein-hrp%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f10780-05","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=19411","title":{"rendered":"A\u578b\u6d41\u611f-NP\u6838\u86cb\u767d\u6297\u9ad4 | Mouse Anti-Influenza A, Nucleoprotein-HRP\u6297\u9ad4\u8ca8\u865f10780-05"},"content":{"rendered":"

Mouse Anti-Influenza A, Nucleoprotein-HRP\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong>10780-05<\/a><\/span><\/p>\n

\u5c0f\u9f20\u6297\u6d41\u611f\u75c5\u6bd2A\u6838\u86cb\u767d<\/strong>\u4ecb\u7d39<\/strong>:<\/strong> \u00a0A\u578b\u6d41\u611f\u75c5\u6bd2\u6838\u86cb\u767d(Influenza virus type A nucleoprotein)\uff0c\u4e5f\u7a31\u70baNP\uff0c\u7531498\u500b\u6c28\u57fa\u9178\u5e8f\u5217\u7d44\u6210\uff0c\u5728\u6d41\u611f\u75c5\u6bd2\u4e2d\u662f\u7279\u5b9a\u985e\u578b\u7684\u3002 NP\u5305\u5c01\u75c5\u6bd2\u57fa\u56e0\u7d44 (NP encapsulates) \u4ee5\u5f62\u6210\u6838\u7cd6\u6838\u86cb\u767d\uff08RNP\uff09\u9846\u7c92\uff0c\u7528\u65bc\u8f49\u9304(transcription)\u548c\u5305\u88dd(packaging)\u3002 \u7279\u7570\u6027\u8b58\u5225A\u578b\u6d41\u611f\u75c5\u6bd2NP\u3002 \u8a72\u514b\u9686\u53ef\u4ee5\u8207\u514b\u9686FluA-NP 2C9\uff08SBA\u8ca8\u865f#10770-01\uff09\u914d\u5c0d\u4f5cELISA\u7684\u6aa2\u6e2c\u6297\u9ad4\u3002<\/p>\n

\u5c0f\u9f20\u6297\u6d41\u611f\u75c5\u6bd2A\u6838\u86cb\u767d<\/strong>\u6297\u9ad4\u7279\u6027: Mouse Anti-Influenza A, Nucleoprotein-UNLB\u6297\u9ad4\u3002\u514d\u75ab\u6e90\u70baRecombinant influenza vrus type A nucleoprotein\u3002\u6297\u9ad4\u5c08\u4e00\u6027\u70baInfluenza virus type A nucleoprotein\u3002\u6297\u9ad4\u6eb6\u6db2Borate buffered saline, pH 8.2\u3002<\/p>\n

\"\"<\/p>\n

Recombinant influenza proteins were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Influenza A, Nucleoprotein-UNLB (SB Cat. No. 10780-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) and chemiluminescent detection.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\nFluA-NP 4F1<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nMouse (BALB\/c) IgG1<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nMouse IgG1<\/sub>-HRP<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nRecombinant influenza virus type A nucleoprotein<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nInfluenza virus type A nucleoprotein<\/td>\n<\/tr>\n
Alternalte Name(s)<\/strong><\/td>\nNP<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nInfluenza virus type A nucleoprotein, also known as NP, is composed of a 498 amino acid sequence and is type-specific in influenza viruses. The NP encapsulates the virus genome to form a ribonucleoprotein (RNP) particle for the purposes of transcription and packaging. The monoclonal antibody FluA-NP 4F1 has been shown to specifically recognize type A influenza virus NP in virus infected cell culture and patient samples. This clone can be paired as a detection antibody for ELISA with clone FluA-NP 2C9 (SB Cat. No. 10770-01).<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nHRP (Horseradish Peroxidase)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\n50% Glycerol\/50% Phosphate buffered saline, pH 7.4<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

ELISA \u2013 Quality tested
\nImmunocytochemistry \u2013 Reported in literature\u00a01-4,6,7<\/sup>
\nWestern Blot \u2013 Reported in literature\u00a02,4,5<\/sup><\/td>\n<\/tr>\n

Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat. No.<\/strong><\/td>\nProduct Description<\/strong><\/td>\nClone<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n<\/thead>\n
10730-05<\/strong><\/a><\/td>\nMouse Anti-Influenza A, Matrix Protein-HRP<\/strong><\/td>\nFluAc<\/td>\n1.0 mL<\/td>\n<\/tr>\n
10730-01<\/strong><\/a><\/td>\nMouse Anti-Influenza A, Matrix Protein-UNLB<\/strong><\/td>\nFluAc<\/td>\n0.1 mg<\/td>\n<\/tr>\n
10770-01<\/strong><\/a><\/td>\nMouse Anti-Influenza A, Nucleoprotein-UNLB<\/strong><\/td>\nFluA-NP 2C9<\/td>\n0.5 mg<\/td>\n<\/tr>\n
10780-05<\/strong><\/a><\/td>\nMouse Anti-Influenza A, Nucleoprotein-HRP<\/strong><\/td>\nFluA-NP 4F1<\/td>\n1.0 mL<\/td>\n<\/tr>\n
10780-01<\/strong><\/a><\/td>\nMouse Anti-Influenza A, Nucleoprotein-UNLB<\/strong><\/td>\nFluA-NP 4F1<\/td>\n0.5 mg<\/td>\n<\/tr>\n
10885-05<\/strong><\/a><\/td>\nMouse Anti-Influenza B, Nucleoprotein-HRP<\/strong><\/td>\n3D7<\/td>\n1.0 mL<\/td>\n<\/tr>\n
10885-01<\/strong><\/a><\/td>\nMouse Anti-Influenza B, Nucleoprotein-UNLB<\/strong><\/td>\n3D7<\/td>\n0.5 mg<\/td>\n<\/tr>\n
10890-05<\/strong><\/a><\/td>\nMouse Anti-Influenza B, Nucleoprotein-HRP<\/strong><\/td>\n1H7a<\/td>\n1.0 mL<\/td>\n<\/tr>\n
10890-01<\/strong><\/a><\/td>\nMouse Anti-Influenza B, Nucleoprotein-UNLB<\/strong><\/td>\n1H7a<\/td>\n0.5 mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6587\u737b\u53c3\u8003<\/p>\n

References<\/p>\n

    \n
  1. Wei G, Meng W, Guo H, Pan W, Liu J, Peng T, et al. Potent neutralization of influenza A virus by a single-domain antibody blocking M2 ion channel protein. PLoS One. 2011;6(12):e28309. (ICC)<\/li>\n
  2. Shoji M, Takahashi E, Hatakeyama D, Iwai Y, Morita Y, Shirayama R, et al. Anti-influenza activity of C60 fullerene derivatives. PLoS One. 2013;8(6):e66337. (ICC, WB)<\/li>\n
  3. Hatakeyama D, Shoji M, Yamayoshi S, Hirota T, Nagae M, Yanagisawa S, et al. A novel functional site in the PB2 subunit of influenza A virus essential for acetyl-CoA interaction, RNA polymerase activity, and viral replication. J Biol Chem. 2014;289:24980-94. (ICC)<\/li>\n
  4. Shoji M, Arakaki Y, Esumi T, Kohnomi S, Yamamoto C, Suzuki Y, et al. Bakuchiol is a phenolic isoprenoid with novel enantiomer-selective anti-influenza A virus activity involving Nrf2 activation. J Biol Chem. 2015 Oct 7. pii: jbc.M115.669465. [Epub ahead of print]. (ICC, WB)<\/li>\n
  5. Narkpuk J, Wanitchang A, Kramyu J, Frantz PN, Jongkaewwattana A, Teeravechyan S. An unconventional BST-2 function: down-regulation of transient protein expression. Biochem Biophys Res Commun. 2014;450:1469-74. (WB)<\/li>\n
  6. Farooqui A, Leon AJ, Lei Y, Wang P, Huang J, Tenorio R, et al. Heterogeneous virulence of pandemic 2009 influenza H1N1 virus in mice. Virol J. 2012;9:104. (ICC)<\/li>\n
  7. Shoji M, Woo S, Masuda A, Win NN, Ngwe H, Takahashi E, et al. Anti-influenza virus activity of extracts from the stems of Jatropha multifida Linn. collected in Myanmar. BMC Complement Altern Med. 2017;17:96. (ICC)<\/li>\n<\/ol>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

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