{"id":19233,"date":"2019-03-14T15:38:51","date_gmt":"2019-03-14T07:38:51","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19233"},"modified":"2022-07-20T14:18:57","modified_gmt":"2022-07-20T06:18:57","slug":"glutamate-carboxypeptidase-ii%e6%8a%91%e5%88%b6%e5%8a%91%e7%af%a9%e9%81%b8%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk440-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=19233","title":{"rendered":"Glutamate Carboxypeptidase II\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2 | \u8ca8\u865fK440-100"},"content":{"rendered":"

Glutamate Carboxypeptidase II<\/strong>\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K440-100<\/a><\/span><\/p>\n

Convenient assay for screening potential GCPII inhibitors<\/em><\/strong><\/p>\n

\"\"\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1aGlutamate Carboxypeptidase II<\/strong>\u8c37\u6c28\u9178\u7fa7\u80bd\u9176II [GCPII\uff0cEC 3.4.17.21; N-\u4e59\u9170\u5316-\u03b1-\u9023\u63a5\u7684\u9178\u6027\u4e8c\u80bd\u9176\uff08N-acetylated-alpha-linked acidic dipeptidase , NAALADase\uff09\uff0c\u524d\u5217\u817a\u7279\u7570\u6027\u819c\u6297\u539f\uff08prostate-specific membrane antigen , PSMA\uff09\u6216\u8449\u9178\u6c34\u89e3\u9176\uff08folate hydrolase , FOLH1\uff09] \u591a\u529f\u80fd\u7684\u92c5 – \u91d1\u5c6c\u80bd\u9176\u3002\u5728\u904e\u53bb\u5341\u5e74\u4e2d\uff0cGCPII\u5df2\u88ab\u7814\u7a76\u4f5c\u70ba\u6cbb\u7642\u5404\u7a2e\u75c5\u7406\u6027\u75be\u75c5\u548c\u75be\u75c5\u7684\u6709\u5e0c\u671b\u7684\u85e5\u7406\u5b78\u9776\u6a19\uff0c\u4e26\u4e14\u5df2\u7d93\u9032\u884c\u4e86\u5de8\u5927\u7684\u52aa\u529b\u4ee5\u767c\u73fe\u6709\u6548\u7684GCPII\u6291\u5236\u5291\u3002 NAALADase\u662f\u4e00\u7a2e\u8166\u540c\u5de5\u9176 (brain isozyme)\uff0c\u5728\u8c37\u6c28\u9178\u751f\u7269\u5408\u6210\u4e2d\u8d77\u8457\u91cd\u8981\u4f5c\u7528\uff0c\u53ef\u7528\u65bc\u6cbb\u7642\u8166\u5167\u8c37\u6c28\u9178\u904e\u91cf\u5f15\u8d77\u7684\u795e\u7d93\u5143\u640d\u50b7\u3002\u4f8b\u5982\uff0cGCPII\u7684\u6291\u5236\u5df2\u7d93\u986f\u793a\u51fa\u5728\u60a3\u6709\u5e7e\u7a2e\u6025\u6027\u548c\u6162\u6027\u75c5\u75c7\uff08\u4e2d\u98a8\uff0c\u795e\u7d93\u6027\u548c\u708e\u6027\u75bc\u75db\uff0c\u7cbe\u795e\u5206\u88c2\u75c7\uff0c\u6291\u9b31\u75c7\u6216\u808c\u840e\u7e2e\u5074\u7d22\u786c\u5316\u75c7\uff09\u7684\u52d5\u7269\u6a21\u578b\u4e2d\u7684\u795e\u7d93\u4fdd\u8b77\u7279\u5fb5\u3002\u5728\u7537\u6027\u4e2d\uff0c PSMA\u5728\u60a3\u6709prostate cancer\u524d\u5217\u817a\u764c\u7684\u60a3\u8005\u4e2d\u904e\u5ea6\u8868\u9054\uff0c\u56e0\u6b64\uff0c\u5b83\u88ab\u7528\u4f5c\u8a72\u75be\u75c5\u7684\u8a3a\u65b7\u6a19\u8a8c\u7269\u3002 BioVision\u7684\u8c37\u6c28\u9178\u7fa7\u80bd\u9176II\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2, \u662f\u4e00\u7a2e\u9ad4\u5916\u5de5\u5177\uff0c\u7528\u65bc\u7be9\u9078\u6f5b\u5728\u7684\u4eba\u985eGCPII\u6291\u5236\u5291\u3002\u63d0\u4f9b\u7684\u5e95\u7269\u5728\u7522\u751fGCPII\u7684\u8c37\u6c28\u9178\u9e7d\u5b58\u5728\u4e0b\u8f49\u6c28\u57fa\u3002\u6aa2\u6e2c\u7cfb\u7d71\u57fa\u65bc\u9176\u4fc3\u53cd\u61c9\uff0c\u5176\u4e2d\u87a2\u5149\u63a2\u91dd\u88ab\u9084\u539f\uff0c\u7522\u751f\u7a69\u5b9a\u7684\u4fe1\u865f\u3002\u57282-PMPA\u5b58\u5728\uff08\u4e00\u7a2e\u6709\u6548\u7684GCPII\u6291\u5236\u5291\uff09\u7684\u60c5\u6cc1\u4e0b\uff0c\u9176\u6d3b\u6027\u88ab\u963b\u6b62\uff0c\u5f9e\u800c\u7522\u751f\u8f03\u4f4e\u7684\u87a2\u5149\u4fe1\u865f\u3002\u8a72\u6aa2\u6e2c\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u7c21\u55ae\u7684\u57fa\u65bc\u5e73\u677f\u7684\u5f62\u5f0f\uff0c\u53ef\u70baGCPII\u6291\u5236\u5291\u7684\u9ad8\u901a\u91cf\u7be9\u9078\u63d0\u4f9b\u5feb\u901f\u53ef\u9760\u7684\u6aa2\u6e2c\u65b9\u6cd5\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK440-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorometric (Ex\/Em= 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nAll<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nScreening\/characterizing Glutamate Carboxypeptidase II inhibitors<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022Includes Inhibitor Control (2-PMPA)
\n\u2022Simple and reliable test to screen GCPII inhibitors\/activators
\n\u2022High-throughput compatible”<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022Human GCPII, 2-PMPA (10 mM)
\n\u2022GCPII Assay Buffer
\n\u2022GCPII Enzyme Mix
\n\u2022PicoProbeTM (in DMSO)
\n\u2022GCPII Developer
\n\u2022 GCPII Substrate<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n
\u00a0Product Name<\/strong><\/td>\nCat.No.<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n
PicoProbe\u2122 Glutaminase (GLS) Activity Assay Kit (Fluorometric)<\/td>\nK455<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric)<\/td>\nK479<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
Glutamine Colorimetric Assay Kit<\/td>\nK556<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
Transglutaminase Activity Assay Kit (Colorimetric)<\/td>\nK571<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
Transglutaminase Inhibitor Screening Assay Kit<\/td>\nK508<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Check out BioVision\u2019s Glutamate related Kits:<\/strong><\/p>\n\n\n\n\n\n\n\n\n
\u00a0Product Name<\/strong><\/td>\nCat.No.<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n
PicoProbe\u2122 Glutamate Assay Kit (Fluorometric)<\/td>\nK413<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
PicoProbe\u2122 Glutamate Carboxypeptidase II Activity Assay Kit<\/td>\nK738<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
Glutamate Carboxypeptidase II Inhibitor Screening Kit (Fluorometric)<\/td>\nK440<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
Glutamate Colorimetric Assay Kit<\/td>\nK629<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
Glutamate Dehydrogenase Activity Colorimetric Assay Kit<\/td>\nK729<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Glutamate Carboxypeptidase II\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2 | \u8ca8\u865fK440-100 Conv […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2308],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19233"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19233"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19233\/revisions"}],"predecessor-version":[{"id":19234,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19233\/revisions\/19234"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19233"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19233"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19233"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}