{"id":19227,"date":"2019-03-13T17:33:02","date_gmt":"2019-03-13T09:33:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19227"},"modified":"2022-07-20T14:19:12","modified_gmt":"2022-07-20T06:19:12","slug":"alpha-1-antitrypsin-a1at-%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fksb697hu01","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=19227","title":{"rendered":"Alpha-1-Antitrypsin (a1AT) \u6aa2\u6e2c\u8a66\u5291\u76d2 |\u8ca8\u865fKSB697Hu01"},"content":{"rendered":"

Uncoated <\/strong>\u591a\u76e4\u5f0f<\/strong>ELISA<\/strong>\u5546\u54c1\u5168\u65b0\u4e0a\u5e02<\/strong>!!<\/strong><\/p>\n

(Uncoated) <\/strong>Alpha-1-Antitrypsin (a1AT) <\/strong>\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/strong>|<\/strong>\u8ca8\u865f<\/strong>KSB697Hu01<\/a><\/span><\/p>\n

ELISA Kit DIY Materials for Alpha-1-Antitrypsin (a1AT)<\/p>\n

\u03b11-\u6297\u80f0\u86cb\u767d\u9176\u662f\u6025\u6027\u671f\u86cb\u767d\u8cea\uff0c\u662f\u7d72\u6c28\u9178\u86cb\u767d\u9176\u6291\u5236\u5291\u8d85\u5bb6\u65cf\u7684\u539f\u578b\u6210\u54e1\u548c\u7d72\u6c28\u9178\u86cb\u767d\u9176\u7684\u4e3b\u8981\u6291\u5236\u5291\u3002 \u4f5c\u70ba\u6025\u6027\u671f\u86cb\u767d\u8cea\uff0c\u03b11-\u6297\u80f0\u86cb\u767d\u9176\u88ab\u8a8d\u70ba\u5728\u9650\u5236\u708e\u75c7\u90e8\u4f4d\u7684\u5bbf\u4e3b\u7d44\u7e54\u640d\u50b7\u4e2d\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002A1AT\u662f52-kDa\u7d72\u6c28\u9178\u86cb\u767d\u9176\u6291\u5236\u5291\uff08\u7d72\u6c28\u9178\u86cb\u767d\u9176\u6291\u5236\u5291\uff09\uff0c\u5728\u91ab\u5b78\u4e0a\u88ab\u8a8d\u70ba\u662f\u6700\u7a81\u51fa\u7684\u7d72\u6c28\u9178\u86cb\u767d\u9176\u6291\u5236\u5291; \u8853\u8a9e\u03b11-\u6297\u80f0\u86cb\u767d\u9176\u548c\u86cb\u767d\u9176\u6291\u5236\u5291\uff08Pi\uff09\u901a\u5e38\u53ef\u4e92\u63db\u4f7f\u7528\u3002\u8207\u6240\u6709\u7d72\u6c28\u9178\u86cb\u767d\u9176\u6291\u5236\u5291\u76f8\u4f3c\uff0cA1AT\u5177\u6709\u03b2\u7247\u548c\u03b1\u87ba\u65cb\u7684\u7279\u5fb5\u4e8c\u7d1a\u7d50\u69cb\u3002 \u9019\u4e9b\u5340\u57df\u7684\u7a81\u8b8a\u53ef\u5c0e\u81f4\u53ef\u5728\u809d\u81df\u4e2d\u805a\u5408\u4f75\u7a4d\u7d2f\u7684\u975e\u529f\u80fd\u6027\u86cb\u767d\u8cea\uff08\u5b30\u5152\u809d\u786c\u5316\uff09\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39:\u00a0<\/strong> (Uncoated) Alpha-1-Antitrypsin (a1AT)\u6aa2\u6e2c\u8a66\u5291\u76d2 (<\/u>\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) <\/u>| Double-antibody Sandwich ELISA\u8ca8\u865fKSB697Hu01\uff0c\u61c9\u7528\u7269\u7a2e\u70baHomo sapiens (Human)\uff1b\u5075\u6e2c\u7684\u9748\u654f\u5ea66.25-400ng\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea62.83ng\/mL, \u53ef\u61c9\u7528\u65bcSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u4e4b\u6a23\u54c1\u4f86\u6e90\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002Cloud-Clone corp\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u4e4bELISA (\u9175\u7d20\u514d\u75ab\u5206\u6790\u5957\u7d44\/\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)\uff0c\u5c08\u9580\u751f\u7522\u7814\u7a76\u7528\u6aa2\u6e2c\u8a66\u5291\u7684\u751f\u6280\u516c\u53f8\uff0c\u65bc\u7f8e\u570b\u7814\u767c\u548c\u751f\u7522\uff0c\u7522\u54c1\u7dda\u5305\u62ecELISA Kit\u3001CLIA Kit\u3001Antibody\u53carecombinant protein\uff1bELISA\u7522\u54c1\u7cfb\u5217\u8d85\u904e6000\u7a2e\u4ee5\u4e0a\uff0c\u7269\u7a2e\u9f4a\u5168\u5305\u62ecrat\u3001mouse\u3001rabbit\u3001cavia\u3001human\u3001porcine\u3001bovine\u3001simian\u7b49\u591a\u7269\u7a2e\u5546\u54c1\u3002\u54c1\u8cea\u4fdd\u8b49\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3001\u9ad8\u9748\u654f\u5ea6\u8a2d\u8a08ELISA\u3002Uncoated<\/u><\/strong> ELISA<\/u><\/strong>\u578b\u5f0f<\/u><\/strong>\uff1b\u5305\u62ecCapture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate\u3002Buffer<\/a>\u7684\u90e8\u4efd\u53e6\u5916\u8cfc\u8cb7\uff0c\u5176\u6bcf\u7d44\u53ef\u9032\u884c5\/10\u76e4ELISA\u5206\u6790\u3002<\/p>\n

Prosaposin (PSAP)<\/strong>\u6aa2\u6e2c\u8a66\u5291\u76d2 (\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)<\/strong>\u5957\u7d44\u5167\u5bb9<\/strong><\/p>\n

\u5305\u62ecCapture Antibody, Detection Antibody\u3001Standard\u3001Streptavidin-HRP\u3001TMB Substrate, 96-well Plate\u3002Buffer<\/a>\u7684\u90e8\u4efd\u53e6\u5916\u8cfc\u8cb7\uff0c\u82e5\u70baSandwich ELISA\u8cc7\u6599\u8acb\u6309\u6b64Assay Kit DIY Support Pack 1<\/a>\uff0ccompetitive method ELISA\u8cc7\u6599\u8acb\u6309\u6b64Assay Kit DIY Support Pack 2<\/a>\u5176\u6bcf\u7d44\u53ef\u9032\u884c10\u76e4ELISA\u5206\u6790\u3002<\/p>\n

Alpha-1-Antitrypsin (a1AT)<\/strong>\u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong>\u64cd\u4f5c\u7c21\u55ae\u6d41\u7a0b<\/strong><\/p>\n

    \n
  1. Coat the plates with 100\u03bcL per well of working solution of Capture Antibody.incubate overnight at 4\u00b0C or incubate at 37\u00b0C for 2 hours.<\/li>\n
  2. Aspirate and wash 1 time.<\/li>\n
  3. Block the plates with 200 \u03bcL per well of working solution of Blocking Buffer. Incubate at 37\u00b0C for 1.5 hours.<\/li>\n
  4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.<\/li>\n<\/ol>\n

    \u00a0<\/strong>Alpha-1-Antitrypsin (a1AT)<\/strong>\u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong>\u76f8\u95dc<\/strong>\u4e4b\u6297\u9ad4 | \u86cb\u767d\u8cea | ELISA | CLIA\u7522\u54c1<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n
    Catalog No.<\/td>\nRelated products for research use of Homo sapiens (Human) Organism species<\/td>\nApplications (RESEARCH USE ONLY!)<\/td>\n<\/tr>\n
    RPB697Hu01<\/td>\nRecombinant Alpha-1-Antitrypsin (a1AT)<\/strong><\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
    PAB697Hu01<\/td>\nPolyclonal Antibody to Alpha-1-Antitrypsin (a1AT)<\/strong><\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
    LAB697Hu71<\/td>\nBiotin-Linked Polyclonal Antibody to Alpha-1-Antitrypsin (a1AT)<\/strong><\/a><\/td>\nWB; IHC; ICC.<\/td>\n<\/tr>\n
    MAB697Hu22<\/td>\nMonoclonal Antibody to Alpha-1-Antitrypsin (a1AT)<\/strong><\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
    SEB697Hu<\/td>\nELISA Kit for Alpha-1-Antitrypsin (a1AT)<\/strong><\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
    SCB697Hu<\/td>\nCLIA Kit for Alpha-1-Antitrypsin (a1AT)<\/strong><\/a><\/td>\nChemiluminescent immunoassay for Antigen Detection.<\/td>\n<\/tr>\n
    KSB697Hu01<\/td>\nELISA Kit DIY Materials for Alpha-1-Antitrypsin (a1AT)<\/strong><\/a><\/td>\nMain materials for “Do It (ELISA Kit) Yourself”.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u00a0<\/strong>\u66f4\u591aDIY Uncoated ELISA\u5206\u6790\u5546\u54c1<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    KSA105Ra01<\/td>\nELISA Kit DIY Materials for Nerve Growth Factor (NGF)<\/td>\n<\/tr>\n
    KSB769Mu01<\/td>\nELISA Kit DIY Materials for S100 Calcium Binding Protein A6 (S100A6)<\/td>\n<\/tr>\n
    KSA543Bo01<\/td>\nELISA Kit DIY Materials for Immunoglobulin M (IgM)<\/td>\n<\/tr>\n
    KSA091Hu01<\/td>\nELISA Kit DIY Materials for Macrophage Derived Chemokine (MDC)<\/td>\n<\/tr>\n
    KSB193Ga01<\/td>\nELISA Kit DIY Materials for Lysozyme (LZM)<\/td>\n<\/tr>\n
    KSA172Mu01<\/td>\nELISA Kit DIY Materials for Platelet Factor 4 (PF4)<\/td>\n<\/tr>\n
    KSA079Mu01<\/td>\nELISA Kit DIY Materials for Interleukin 6 (IL6)<\/td>\n<\/tr>\n
    KSA480Hu01<\/td>\nELISA Kit DIY Materials for Myoglobin (MYO)<\/td>\n<\/tr>\n
    KSA785Hu01<\/td>\nELISA Kit DIY Materials for Kidney Injury Molecule 1 (Kim1)<\/td>\n<\/tr>\n
    KSA601Hu01<\/td>\nELISA Kit DIY Materials for Myeloperoxidase (MPO)<\/td>\n<\/tr>\n
    KSA124Mu01<\/td>\nELISA Kit DIY Materials for Transforming Growth Factor Beta 1 (TGFb1)<\/td>\n<\/tr>\n
    KSA820Ra01<\/td>\nELISA Kit DIY Materials for Coagulation Factor II (F2)<\/td>\n<\/tr>\n
    KSA124Ra01<\/td>\nELISA Kit DIY Materials for Transforming Growth Factor Beta 1 (TGFb1)<\/td>\n<\/tr>\n
    KSA785Ra01<\/td>\nELISA Kit DIY Materials for Kidney Injury Molecule 1 (Kim1)<\/td>\n<\/tr>\n
    KSB522Hu01<\/td>\nELISA Kit DIY Materials for Pulmonary Activation Regulated Chemokine (PARC)<\/td>\n<\/tr>\n
    KSA124Hu01<\/td>\nELISA Kit DIY Materials for Transforming Growth Factor Beta 1 (TGFb1)<\/td>\n<\/tr>\n
    KSB388Hu01<\/td>\nELISA Kit DIY Materials for Neutrophil Gelatinase Associated Lipocalin (NGAL)<\/td>\n<\/tr>\n
    KSA091Mu01<\/td>\nELISA Kit DIY Materials for Macrophage Derived Chemokine (MDC)<\/td>\n<\/tr>\n
    KSA853Hu01<\/td>\nELISA Kit DIY Materials for Caspase 8 (CASP8)<\/td>\n<\/tr>\n
    KSA123Hu01<\/td>\nELISA Kit DIY Materials for Transforming Growth Factor Alpha (TGFa)<\/td>\n<\/tr>\n
    KSA785Mu01<\/td>\nELISA Kit DIY Materials for Kidney Injury Molecule 1 (Kim1)<\/td>\n<\/tr>\n
    KSA394Mu01<\/td>\nELISA Kit DIY Materials for Tissue Factor Pathway Inhibitor (TFPI)<\/td>\n<\/tr>\n
    KSA234Hu01<\/td>\nELISA Kit DIY Materials for Immunoglobulin G4 (IgG4)<\/td>\n<\/tr>\n
    KSA110Hu01<\/td>\nELISA Kit DIY Materials for Oncostatin M (OSM)<\/td>\n<\/tr>\n
    KSA029Mu01<\/td>\nELISA Kit DIY Materials for Selectin, Endothelium (SELE)<\/td>\n<\/tr>\n
    KSA145Hu01<\/td>\nELISA Kit DIY Materials for Vascular Endothelial Growth Factor C (VEGFC)<\/td>\n<\/tr>\n
    KSC036Hu01<\/td>\nELISA Kit DIY Materials for Transferrin (TRF)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Uncoated \u591a\u76e4\u5f0fELISA\u5546\u54c1\u5168\u65b0\u4e0a\u5e02!! (Uncoated) Alpha-1-Antitrypsi […]<\/p>\n","protected":false},"author":3,"featured_media":16326,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[462],"tags":[2310],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19227"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19227"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19227\/revisions"}],"predecessor-version":[{"id":19228,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19227\/revisions\/19228"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16326"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19227"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19227"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19227"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}