{"id":19107,"date":"2019-02-26T14:14:14","date_gmt":"2019-02-26T06:14:14","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=19107"},"modified":"2024-03-01T19:14:41","modified_gmt":"2024-03-01T11:14:41","slug":"phi29-dna-polymerase%e8%81%9a%e5%90%88%e9%85%b6-mesophilic-dna-polymerases-%e8%b2%a8%e8%99%9fe014-1000u100ul","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=19107","title":{"rendered":"Phi29 DNA Polymerase\u805a\u5408\u9176 | Mesophilic DNA Polymerases \u8ca8\u865fE014 (1000U\/100ul)"},"content":{"rendered":"

Phi29 DNA Polymerase<\/strong>\u805a\u5408\u9176 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>E014 (1000U\/100ul)<\/a><\/span><\/p>\n

A highly processive DNA polymerase which exhibits a strong strand-displacement function<\/em><\/strong><\/p>\n

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\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>Phi29 DNA\u805a\u5408\u9176\u662f\u4e00\u7a2e\u9ad8\u5ea6\u52a0\u5de5\u7684polymerase\u805a\u5408\u9176 \uff08\u9ad8\u905470 kb\u4ee5\u4e0a\uff09\uff0c\u5177\u6709\u5f88\u5f37\u7684strand displacement activity\u93c8\u63db\u7f6e\u63db\u6d3b\u6027\u3002phi29 DNA\u805a\u5408\u9176\u9084\u5177\u6709\u512a\u5148\u4f5c\u7528\u65bc\u55ae\u93c8DNA\u6216RNA\u76843’\u21925’\u6838\u9178\u5916\u5207\u9176\uff08proofreading\uff09\u6d3b\u6027\u3002\u56e0\u6b64\u5f37\u70c8\u63a8\u85a63′-modified\u7684primers\u5f15\u7269\u00a0Phi29 DNA Polymerase<\/strong>\u805a\u5408\u9176<\/strong>\u61c9\u7528\u00a01.\u00a0\u00a0\u00a0\u00a0\u00a0 Rolling circle amplification (RCA)\uff1a\u7522\u751fperiodic DNA nanotemplates2.\u00a0\u00a0\u00a0\u00a0\u00a0 \u591a\u4f4d\u79fb\u64f4\u589eMultiple displacement amplification\uff08MDA\uff093.\u00a0\u00a0\u00a0\u00a0\u00a0 Unbiased amplification\u7121\u57fa\u56e0\u64f4\u589e\u5168\u57fa\u56e0\u7d44 (WGA)4.\u00a0\u00a0\u00a0\u00a0\u00a0 \u7528\u65bcSNP\u548cSTR\u6aa2\u6e2c\u64f4\u589eDNA5.\u00a0\u00a0\u00a0\u00a0\u00a0 Protein-primed DNA\u64f4\u589e\u3002<\/p>\n

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<\/p>\n\n\n\n\n\n\n\n\n\n
Description<\/strong><\/td>\nPhi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and\/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3\u2019\u21925\u2019 exonuclease activity and can amplify from very small amounts of starting templates.<\/td>\n<\/tr>\n
Application<\/strong><\/td>\n\u2022 Rolling circle amplification (RCA)
\n\u2022 Multiple displacement amplification (MDA)
\n\u2022 Whole genome amplification (WGA)
\n\u2022 DNA template preparation for sequencing
\n\u2022 Protein-primed DNA amplification<\/td>\n<\/tr>\n
Form<\/strong><\/td>\nEnzyme supplied with 5X Reaction Buffer<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n10 U\/\u03bcl<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nStore all components at -20\u00b0C.<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.5% Tween-20, 0.5% NP-40 and 50% (v\/v) Glycerol.<\/td>\n<\/tr>\n
Notes<\/strong><\/td>\n\n

One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30\u00b0C.<\/p>\n

This product is distributed for laboratory research only.
\nCaution: Not for diagnostic use .<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Phi29 DNA Polymerase\u805a\u5408\u9176 | \u8ca8\u865fE014 (1000U\/100ul) A highly […]<\/p>\n","protected":false},"author":3,"featured_media":2030,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373,88],"tags":[2332],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19107"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19107"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19107\/revisions"}],"predecessor-version":[{"id":48879,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/19107\/revisions\/48879"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/2030"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19107"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19107"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19107"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}