{"id":18855,"date":"2019-02-13T16:36:17","date_gmt":"2019-02-13T08:36:17","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18855"},"modified":"2022-07-20T14:38:07","modified_gmt":"2022-07-20T06:38:07","slug":"active-tumor-necrosis-factor-alpha%e8%85%ab%e7%98%a4%e5%a3%9e%e6%ad%bb%e5%9b%a0%e5%ad%90%ce%b1%ef%bc%88tnfa%ef%bc%89%e6%b4%bb%e6%80%a7%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-%e8%b2%a8%e8%99%9fapa133hu01","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=18855","title":{"rendered":"\u816b\u7624\u58de\u6b7b\u56e0\u5b50\u03b1\uff08TNFa\uff09\u6d3b\u6027\u91cd\u7d44\u86cb\u767d |\u8ca8\u865fAPA133Hu01"},"content":{"rendered":"

\u9644<\/em><\/strong>Protein marker <\/em><\/strong>\u6216<\/em><\/strong> positive control <\/em><\/strong><\/p>\n

Active Tumor Necrosis Factor Alpha<\/strong>\u816b\u7624\u58de\u6b7b\u56e0\u5b50\u03b1\uff08TNFa\uff09<\/strong>\u6d3b\u6027<\/strong>\u91cd\u7d44\u86cb\u767d <\/strong>|<\/strong>\u8ca8\u865f<\/strong>APA133Hu01<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Cloud clone\u63d0\u4f9b\u539f\u6838\u53ca\u771f\u6838\u751f\u7269\u8868\u73fe\u4e4b\u91cd\u7d44\u86cb\u767d\u7cfb\u5217\u5546\u54c1\uff1b\u5176\u4e2dActive Tumor Necrosis Factor Alpha\u6d3b\u6027\u91cd\u7d44\u86cb\u767d\uff0c\u70ba\u6d3b\u6027\u86cb\u767d\u3002\u7d14\u5ea6> 95%\uff0c\u63d0\u4f9b\u51cd\u4e7e\u578b\u5f0f\u5305\u88dd\u3002\u53ef\u61c9\u7528\u65bcCell culture; Activity Assays.\u7b49\u4e0d\u540c\u7814\u7a76\u3002 Organism Species Homo sapiens (Human)<\/p>\n

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TNFa (Tumor necrosis factor), is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It has been reported that TNFa can inhibit the proliferation and induce apoptosis of A549 cells, besides, the concentration of IL-1\u03b2 and IL-8 in cell supernatant will increase after stimulation. Therefore, a stimulation assay of TNFa was conducted using A549 cells. Briefly, A549 cells were incubated in DMEM with different concentrations of TNFa (1ng\/mL, 10ng\/mL, 100ng\/mL, 1000ng\/mL) for 8h, after which the concentration of IL-1\u03b2 and IL-8 in the cell supernatant were detected by ELISA. IL-1\u03b2 and IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1\u03b2, the data was shown in Figure 1 and Figure 2 separately.<\/p>\n

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Figure. Western Blot; Sample: Recombinant TNFa, Human.<\/p>\n

\u86cb\u767d\u8cea\u4fdd\u5b58\u578b\u5f0f<\/strong><\/p>\n

\u86cb\u767d\u8cea\u6eb6\u65bc20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300 \u70ba\u51b7\u51cd\u4e7e\u71e5\u5305\u88dd\u3002\u4f7f\u7528\u524dReconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg\/mL.\u907f\u514dvortex. \u77ed\u671f\u4fdd\u5b58Store at 2-8\u00b0C for one month.\u5206\u88dd-80\u00b0C\u9577\u671f12 \u500b\u6708\u3002\u8acb\u907f\u514d\u53cd\u8986\u51b7\u51cd\u89e3\u51cd\u3002.<\/p>\n

\"APA133Hu01.jpg\"<\/p>\n

(GDNF)\u6d3b\u6027\u91cd\u7d44\u86cb\u767d\u76f8\u95dc\u4e4b\u6297\u9ad4<\/strong> | <\/strong>\u86cb\u767d\u8cea<\/strong> \u7522\u54c1<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Catalog No.<\/td>\nRelated products for research use of Homo sapiens (Human) Organism species<\/td>\nApplications (RESEARCH USE ONLY!)<\/td>\n<\/tr>\n
APA133Hu01<\/td>\nActive Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nCell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n
RPA133Hu01<\/td>\nRecombinant Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
PAA133Hu01<\/td>\nPolyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
LAA133Hu81<\/td>\nFITC-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nWB; IHC; ICC; IF.<\/td>\n<\/tr>\n
LAA133Hu71<\/td>\nBiotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nWB; IHC; ICC.<\/td>\n<\/tr>\n
MAA133Hu22<\/td>\nMonoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
MAA133Hu21<\/td>\nMonoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
LAA133Hu72<\/td>\nBiotin-Linked Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nWB; IHC; ICC.<\/td>\n<\/tr>\n
MEA133Hu<\/td>\nMini Samples ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
SEA133Hu<\/td>\nELISA Kit for Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
AEA133Hu<\/td>\nELISA Kit for Anti-Tumor Necrosis Factor Alpha Antibody (Anti-TNFa)<\/strong><\/a><\/td>\nEnzyme-linked immunosorbent assay for Antibody Detection.<\/td>\n<\/tr>\n
HEA133Hu<\/td>\nHigh Sensitive ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
SCA133Hu<\/td>\nCLIA Kit for Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nChemiluminescent immunoassay for Antigen Detection.<\/td>\n<\/tr>\n
PSA133Hu01<\/td>\nAntibody Pair for Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.<\/td>\n<\/tr>\n
KSA133Hu01<\/td>\nELISA Kit DIY Materials for Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nMain materials for “Do It(ELISA Kit) Yourself”<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u9644Protein marker \u6216 positive control Active Tumor Necrosi […]<\/p>\n","protected":false},"author":3,"featured_media":16383,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[461],"tags":[2380],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18855"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18855"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18855\/revisions"}],"predecessor-version":[{"id":18862,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18855\/revisions\/18862"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16383"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18855"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18855"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18855"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}