{"id":18714,"date":"2019-01-18T10:38:44","date_gmt":"2019-01-18T02:38:44","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18714"},"modified":"2023-03-09T18:15:12","modified_gmt":"2023-03-09T10:15:12","slug":"perfringens-agar-tsc-%e7%94%a2%e6%b0%a3%e5%b1%a4%e7%93%8a%e8%84%82%e5%9f%b9%e9%a4%8a%e5%9f%ba-%e8%b2%a8%e8%99%9f-lab194","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=18714","title":{"rendered":"Perfringens Agar (TSC) \u7522\u6c23\u5c64\u74ca\u8102\u57f9\u990a\u57fa | \u8ca8\u865f NCM0077A \/LAB194"},"content":{"rendered":"

Perfringens Agar (TSC) \u7522\u6c23\u5c64\u74ca\u8102\u57f9\u990a\u57fa<\/strong> | <\/strong>\u8ca8\u865fNCM0077A\/<\/strong>LAB194<\/strong><\/a><\/span><\/p>\n

\"NEOGEN\u5546\u54c1\u5716\"<\/p>\n

Clostridium perfringens\u7522\u6c23\u83a2\u819c\u68ad\u72c0\u82bd\u5b62\u687f\u83cc\u57f9\u990a<\/span><\/p>\n

Perfringens Agar Base\u7522\u6c23\u83a2\u819c\u74ca\u8102\u57f9\u990a\u57fa\u662f\u4e00\u7a2e\u71df\u990a\u57f9\u990a\u57fa\uff0c\u5176\u4e2d\u52a0\u5165egg yolk emulsion\u86cb\u9ec3\u4e73\u6db2\uff08X073\uff09\/ (Egg Yolk Tellurite Emulsion (50 ml\/100 ml per vial)<\/a>\u00a0HIMEDIA\u8ca8\u865fFD046)<\/span> \u548ccycloserine\u74b0\u7d72\u6c28\u9178\uff08X194\/NEOGEN NCM4022-0.5<\/a><\/span>\uff09\u7528\u65bc\u88fd\u5099\u80f0\u86cb\u767d\u8156\u4e9e\u786b\u9178\u9e7d\u74b0\u7d72\u6c28\u9178\uff08TSC\uff09\u74ca\u8102\u3002Sodium metabisulphite\u504f\u4e9e\u786b\u9178\u6c2b\u9209\u548cferric ammonium citrate\u6ab8\u6aac\u9178\u9435\u92a8\u7528\u4f5cClostridium perfringens\u7522\u6c23\u83a2\u819c\u68ad\u83cc\uff08Clostridium perfringens\uff09\u6e1b\u5c11sulphite reduction\u4e9e\u786b\u9178\u9e7d\u7684\u6307\u6a19\u3002 \u901a\u904eC.perfringens\u6e1b\u5c11\u4e9e\u786b\u9178\u9e7d\u7522\u751f\u9ed1\u8272\u83cc\u843d\uff0c\u4e26\u4e14\u647b\u5165\u57f9\u990a\u57fa\u4e2d\u7684\u86cb\u9ec3\u4e73\u6db2\u6aa2\u6e2c\u8a72\u7d30\u83cc\u7684\u5375\u78f7\u8102\u9176\u6d3b\u6027\u3002 \u7136\u800c\uff0c\u4e26\u975e\u6240\u6709\u83cc\u682a\u90fd\u7522\u751f\u5375\u78f7\u8102\u9176\uff0c\u56e0\u6b64\u9ed1\u8272\u5375\u78f7\u8102\u9176\u967d\u6027\u548c\u9ed1\u8272\u5375\u78f7\u8102\u9176\u9670\u6027\u83cc\u843d\u61c9\u88ab\u8996\u70ba\u63a8\u5b9a\u7684C.perfringens\u3002<\/p>\n

Minimum Q.C. organisms: Clostridium perfringens WDCM 00007 Escherichia coli WDCM 00013 (inhibition)<\/p>\n

\u7522\u6c23\u5c64\u74ca\u8102<\/strong>\u57f9\u990a\u57fa<\/strong>Base<\/strong>\u914d\u65b9<\/strong> Typical Formula g\/litre <\/strong><\/span><\/p>\n

Tryptose——————————————————– 15.0<\/p>\n

Soy Peptone —————————————————–5.0<\/p>\n

Beef extract—————————————————– 5.0<\/p>\n

Yeast extract—————————————————— 5.0<\/p>\n

Sodium metabisulphite—————————————- 1.0<\/p>\n

Ferric ammonium citrate—————————————- 1.0<\/p>\n

Agar—————————————————————-14.0<\/p>\n

 <\/p>\n

\u57f9\u990a\u57fa\u4f7f\u7528\u65b9\u6cd5<\/strong>:<\/strong> <\/span>\u7a31\u53d641.0\u514b\u7c89\u672b\u4e26\u5206\u6563\u5728900ml\u53bb\u96e2\u5b50\u6c34\u4e2d\u3002 \u8b93\u6df7\u5408\u7269\u6d78\u6ce110\u5206\u9418\uff0c\u65cb\u8f49\u6df7\u5408\u4f75\u6d88\u6bd2\uff1b\u901a\u904e\u5728121\u2103\u4e0b\u9ad8\u58d3\u6ec5\u83cc15\u5206\u9418\u3002 \u8b93\u57f9\u990a\u57fa\u51b7\u537b\u81f345-50\u00b0C\u4e26\u88dc\u51452\u74f6X194\uff08X194\/NEOGEN NCM4022-0.5<\/a>\uff09\u548c\u00a0100 mL of NCM4017* Egg Yolk Emulsion, 50%\u652a\u62cc\u5747\u52fb\uff0c\u5012\u5165\u7121\u83cc\u57f9\u990a\u76bf\u4e2d\u3002 \u7701\u7565\u86cb\u9ec3\u4e73\u6db2\u7528\u65bc\u88fd\u5099\u7121\u86cb\u9ec3\u3002<\/p>\n

    \n
  1. Suspend 41 grams of the medium in 900 mL of purified water.<\/li>\n
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.<\/li>\n
  3. Autoclave at 121\u00b0C for 15 minutes. Cool to 45 – 50\u00b0C.<\/li>\n
  4. Add 100 mL of NCM4017* Egg Yolk Emulsion, 50%, and the desired antimicrobic supplement: TSC Agar: Add 2 vials of NCM4022-0.5* TSC Perfringens Supplement, each reconstituted using 5 mL sterile deionized\/RO water.<\/li>\n<\/ol>\n

    Overlay Agar<\/span><\/strong><\/span><\/h4>\n
      \n
    1. Suspend 41 grams of the medium in one liter of purified water.<\/li>\n
    2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.<\/li>\n
    3. Autoclave at 121\u00b0C for 15 minutes. Cool to 45 – 50\u00b0C.<\/li>\n
    4. Overlay agar does not get supplemented with Egg Yolk Emulsion. Overlay agar is held at 45 -50\u00b0C until used and should be poured right after inoculation.<\/li>\n<\/ol>\n

      References<\/span><\/strong><\/span><\/p>\n

      Shahidi, S.A. and Furguson, A.R. (1971). Appl. Microbiol. 21. 500-506.<\/p>\n

      Harmon, S.M., Kauttar, D.A. and Peeler, J.T. (1971). Appl. Microbiol. 22. 688-692.<\/p>\n

      Hauschild, A. H. W. and Hilsheimar R. (1973). Appl. Microbiol. 27. 78-82.<\/p>\n

      Hauschild A.H.W. and Hilsheimar, R. (1973). Appl. Microbiol. 27. 521-526.<\/p>\n

      Hauschild, A.H.W. et al (1977). Can. J. Microbiol. 23. 884-892.<\/p>\n

      Labbe, R. G. and Harmon, S.M. (1992). Compendium of methods for the microbiological examination of foods, 3rd ed 623-635.
      \n      TSC Perfringens Supplement\u7522\u6c23\u83a2\u819c\u68ad\u83cc\u7684\u9078\u64c7\u6027\u57f9\u990a\u57fa\u88dc\u5145\u5291 |\u00a0\u8ca8\u865fNCM4022-0.5\u00a0<\/a><\/span><\/strong><\/p>\n

      \"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
      Specifications<\/th>\n<\/td>\n<\/tr>\n
      Brand<\/td>\nNeogen\u00ae Culture Media<\/td>\n<\/tr>\n
      Platform<\/td>\nDehydrated Culture Media<\/td>\n<\/tr>\n
      Formula<\/td>\nCycloserine – 400 mg\/L<\/td>\n<\/tr>\n
      Preparation<\/td>\nNo preparation required. Ready to use.<\/td>\n<\/tr>\n
      Previous Item Number<\/td>\nProduct was previously known as Abtek product: #07-IS\/CP3 Cycloserine for TSC Agar; and Lab M\u00ae product: #X194 D-Cycloserine Supplement<\/td>\n<\/tr>\n
      Storage Conditions<\/td>\n2-8\u00b0C<\/td>\n<\/tr>\n
      Volume<\/td>\n500<\/td>\n<\/tr>\n
      Quantity per Package<\/td>\n10<\/td>\n<\/tr>\n
      Package Weight<\/td>\n0.66 lb<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \"\"<\/p>\n","protected":false},"excerpt":{"rendered":"

      Perfringens Agar (TSC) \u7522\u6c23\u5c64\u74ca\u8102\u57f9\u990a\u57fa | \u8ca8\u865fNCM0077A\/LAB194 Clo […]<\/p>\n","protected":false},"author":3,"featured_media":18715,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[88],"tags":[2411],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18714"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18714"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18714\/revisions"}],"predecessor-version":[{"id":39753,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18714\/revisions\/39753"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/18715"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18714"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18714"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18714"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}