{"id":18397,"date":"2018-12-17T15:55:27","date_gmt":"2018-12-17T07:55:27","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18397"},"modified":"2022-07-20T15:12:49","modified_gmt":"2022-07-20T07:12:49","slug":"mirna-cdna%e5%90%88%e6%88%90%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fg269g270","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=18397","title":{"rendered":"miRNA cDNA\u5408\u6210\u8a66\u5291\u76d2 | \u8ca8\u865fG269\/G270"},"content":{"rendered":"

miRNA cDNA<\/strong>\u5408\u6210\u8a66\u5291\u76d2<\/strong>\u00a0|\u00a0<\/strong>\u8ca8\u865f\u00a0<\/strong>G269\/G270<\/a><\/span><\/p>\n

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\u7522\u54c1\u4ecb\u7d39<\/strong>:\u00a0<\/strong>miRNA cDNA\u5408\u6210\u8a66\u5291\u76d2\u662f\u5f9etotal RNA\u6a21\u677f\u4e2d\u6709\u6548\u5408\u6210\u7b2c\u4e00\u93c8miRNA\u7684\u5b8c\u6574\u7cfb\u7d71\u3002\u8a72\u8a66\u5291\u76d2\u5229\u7528\u6700\u4f73\u5316\u4e4b\u7a81\u8b8a\u91cd\u7d44M-MuLV\u9006\u8f49\u9304\u9176\uff0c\u6709\u6548limited\u00a0 RNA\u9176H\u6d3b\u6027\u3002\u8a66\u5291\u76d2\u63d0\u4f9b\u7684\u91cd\u7d44RNasin\u2122\u6838\u7cd6\u6838\u9178\u9176\u6291\u5236\u5291, \u53ef\u6709\u6548\u4fdd\u8b77RNA\u6a21\u677f\u514d\u65bc\u964d\u89e3\u3002\u9664\u4e86\u57fa\u672c\u7684miRNA cDNA\u5408\u6210\u8a66\u5291\u76d2\uff08G269 \/ G270\uff09\u5916\uff0c\u5ba2\u6236\u9084\u53ef\u4ee5\u9078\u64c7\u5e36\u6709Poly\uff08A\uff09Polymerase Tailing\uff08G902 \/ G903\uff09\u7684miRNA cDNA\u5408\u6210\u8a66\u5291\u76d2\u3002 abm\u7684miRNA cDNA\u5408\u6210\u8a66\u5291\u76d2\u7684\u5169\u7a2e\u7248\u672c\u90fd\u8207oligo d\uff08T\uff09\u929c\u63a5\u982d\u4e00\u8d77\uff0c\u5b83\u5011\u9078\u64c7\u6027\u5230poly\uff08A\uff09\u5c3e\u90e8\u7684miRNA\u4e0a\u3002\u5305\u542b\u5728miRNA\u5206\u6790\u8a66\u5291\u76d2\uff08MA003\uff0cMA004\uff09\u4e2d\u7684\u901a\u7528\u53cd\u5411\u5f15\u7269\u542b\u6709\u929c\u63a5\u5b50\u5e8f\u5217\uff0c\u56e0\u6b64\u5b83\u5011\u5fc5\u9808\u7528\u65bc\u7531abm\u7684miRNA cDNA\u5408\u6210\u8a66\u5291\u76d2\u7522\u751f\u7684cDNA\u3002 miRNA\u7684\u7b2c\u4e00\u689dcDNA\u53ef\u76f4\u63a5\u4f5c\u70ba\u6a21\u677f\u7528\u65bc\u7d66\u5b9a\u6a23\u54c1\u4e2dmiRNA\u8868\u9054\u7684\u57fa\u65bcqPCR\u7684\u5206\u6790\u3002 abm\u4e5f\u5177\u6709\u7528\u65bc\u6240\u6709\u5df2\u77e5miRNA\u5e8f\u5217\u7684miRNA\u5f15\u7269\u7684\u5b8c\u6574\u6587\u5eab\u3002\u66f4\u591a\u8cc7\u6599\u8acb\u6309\u6b64\u00a0miRNA primer<\/a>\u00a0<\/span><\/span><\/p>\n\n\n\n\n\n\n\n
Product<\/strong><\/td>\nDescription<\/strong><\/td>\nCat No.<\/strong><\/td>\n<\/tr>\n
miRNA cDNA Synthesis Kit<\/td>\n25 reactions<\/td>\nG269<\/td>\n<\/tr>\n
miRNA cDNA Synthesis Kit<\/td>\n100 reactions<\/td>\nG270<\/td>\n<\/tr>\n
miRNA cDNA Synthesis Kit, with Poly(A) Polymerase Tailing<\/td>\n25 reactions<\/td>\nG902<\/td>\n<\/tr>\n
miRNA cDNA Synthesis Kit, with Poly(A) Polymerase Tailing<\/td>\n100 reactions<\/td>\nG903<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Lang, B\u00a0et al<\/i>. “miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4\/BCAS2.” Oncology Reports 39:71-80 (2018).\u00a0DOI: 10.3892\/or.2017.6064<\/a>.<\/p>\n

Kawakubo-Yasukochi, T\u00a0et al<\/i>. “miR-200c-3p spreads invasive capacity in human oral squamous cell carcinoma microenvironment.” Molecular Carcinogenesis 57 (2):295-302 (2018).\u00a0DOI: 10.1002\/mc.22744<\/a>.<\/p>\n

Qin, X\u00a0et al<\/i>. “Upregulation of DARS2 by HBV promotes hepatocarcinogenesis through the miR-30e-5p\/MAPK\/NFAT5 pathway.” J. Exp. Clin. Cancer Res. 148: (2017).\u00a0DOI: 10.1186\/s13046-017-0618-x<\/a>.<\/p>\n

Application: PCR.Shen, C\u00a0et al<\/i>. “Comparative analysis of cadmium responsive microRNAs in roots of two Ipomoea aquatica Forsk. cultivars with different cadmium accumulation capacities.” Plant Physiology and Biochemistry 111:329-339 (2017).\u00a0DOI: 10.1016\/j.plaphy.2016.12.013<\/a>.<\/p>\n

Cheng, F\u00a0et al<\/i>. “RNA-Binding Protein Dnd1 Promotes Breast Cancer Apoptosis by Stabilizing the Bim mRNA in a miR-221 Binding Site.” BioMed Research International : (2017).\u00a0DOI: 10.1155\/2017\/9596152<\/a>.<\/p>\n

Wu, L\u00a0et al<\/i>. “Clinical significance of C\/D box small nucleolar RNA U76 as an oncogene and a prognostic biomarker in hepatocellular carcinoma.” Clin Res Hepatol Gastroenterol : (2017).\u00a0PubMed: 10.1016\/j.clinre.2017.04.018<\/a>.<\/p>\n

Ramraj,\u00a0et al<\/i>. “Serum-circulating miRNAs predict neuroblastoma progression in mouse model of high-risk metastatic disease.” Oncotarget 7.14:18605 (2016).\u00a0DOI: 10.18632\/oncotarget.7615<\/a>. Application: miRNA qPCR Analysis.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

miRNA cDNA\u5408\u6210\u8a66\u5291\u76d2\u00a0|\u00a0\u8ca8\u865f\u00a0G269\/G270 \u7522\u54c1\u4ecb\u7d39:\u00a0miRNA cDNA\u5408\u6210\u8a66\u5291\u76d2\u662f\u5f9et […]<\/p>\n","protected":false},"author":3,"featured_media":5254,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[132],"tags":[2464],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18397"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18397"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18397\/revisions"}],"predecessor-version":[{"id":18400,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18397\/revisions\/18400"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/5254"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18397"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18397"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18397"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}