{"id":18058,"date":"2018-10-19T12:00:02","date_gmt":"2018-10-19T04:00:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18058"},"modified":"2022-07-20T15:28:41","modified_gmt":"2022-07-20T07:28:41","slug":"%e4%b8%99%e9%85%ae%e9%85%b8%e6%bf%80%e9%85%b6%e6%b4%bb%e6%80%a7%e6%af%94%e8%89%b2%e8%9e%a2%e5%85%89%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk709","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=18058","title":{"rendered":"\u4e19\u916e\u9178\u6fc0\u9176\u6d3b\u6027\u6bd4\u8272\/\u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fK709"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=18058\">\u4e19\u916e\u9178\u6fc0\u9176\u6d3b\u6027\u6bd4\u8272\/\u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 <strong>| <\/strong><strong>\u8ca8\u865f<\/strong>K709<\/a><\/span><\/p>\n<p><strong><em>Pyruvate Kinase Activity Colorimetric\/Fluorometric Assay Kit<\/em><\/strong><\/p>\n<p><strong>\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u4e19\u916e\u9178\u6fc0\u9176\uff08PK\uff0cEC 2.7.1.40\uff09\u662f\u53c3\u8207\u7cd6\u9175\u89e3\u7684\u9176\u3002 \u5b83\u50ac\u5316\u78f7\u9178\u57fa\u5718\u5f9e\u78f7\u9178\u70ef\u9187\u4e19\u916e\u9178\uff08PEP\uff09\u8f49\u79fb\u5230ADP\uff0c\u7522\u751f\u4e00\u5206\u5b50\u4e19\u916e\u9178\u548c\u4e00\u5206\u5b50ATP\u3002 \u7f3a\u4e4f\u4e19\u916e\u9178\u6fc0\u9176\u6703\u6e1b\u7de9\u7cd6\u9175\u89e3\u904e\u7a0b\uff0c\u5c0e\u81f4\u7a31\u70ba\u4e19\u916e\u9178\u6fc0\u9176\u7f3a\u4e4f\u75c7\u7684\u75be\u75c5\u3002 BioVision\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\uff0c\u76f4\u63a5\u548c\u81ea\u52d5\u5316\u7684\u65b9\u6cd5\uff0c\u7528\u65bc\u6e2c\u91cf\u5404\u7a2e\u751f\u7269\u6a23\u54c1\uff08\u5982\u8840\u6db2\uff0c\u7d44\u7e54\u548c\u57f9\u990a\u7d30\u80de\u7b49\uff09\u4e2d\u7684\u4e19\u916e\u9178\u6fc0\u9176\u6d3b\u6027\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cPEP\u548cADP\u901a\u904ePK\u50ac\u5316\u7522\u751f\u4e19\u916e\u9178\u548cATP\u3002 \u7522\u751f\u7684\u4e19\u916e\u9178\u88ab\u4e19\u916e\u9178\u6c27\u5316\u9176\u6c27\u5316\uff0c\u7522\u751f\u984f\u8272\uff08\u03bb= 570nm\uff09\u548c\u7192\u5149\uff08Ex \/ Em = 535 \/ 587nm\uff09\u3002 \u7531\u65bc\u984f\u8272\u6216\u87a2\u5149\u5f37\u5ea6\u7684\u589e\u52a0\u8207\u4e19\u916e\u9178\u91cf\u7684\u589e\u52a0\u6210\u6bd4\u4f8b\uff0c\u56e0\u6b64\u53ef\u4ee5\u7cbe\u78ba\u5730\u6e2c\u91cfPK\u6d3b\u6027\u3002 \u8a72\u8a66\u5291\u76d2\u6aa2\u6e2c0.1 mU \/ ml\u4e19\u916e\u9178\u6fc0\u9176\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/7\/k709.jpg\" alt=\"\" \/><\/p>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Cat # +Size<\/strong><\/td>\n<td>K709-100<\/td>\n<\/tr>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>100 assays<\/td>\n<\/tr>\n<tr>\n<td><strong>Detection Method<\/strong><\/td>\n<td>Absorbance (570 nm) or Fluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n<tr>\n<td><strong>Species Reactivity<\/strong><\/td>\n<td>Mammalian<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>The kit detects 0.1 mU\/ml pyruvate kinase.<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>\u2022 Simple procedure; takes ~ less than 40 minutes<br \/>\n\u2022 Fast and convenient<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 PK Assay Buffer<br \/>\n\u2022 OxiRed\u2122 Probe (in DMSO)<br \/>\n\u2022 PK Enzyme Mix<br \/>\n\u2022 PK Substrate Mix<br \/>\n\u2022 PK Positive Control (~18 mU)<br \/>\n\u2022 Pyruvate Standard (100 nmol\/\u00b5l)<\/td>\n<\/tr>\n<tr>\n<td><strong>Storage Conditions<\/strong><\/td>\n<td>-20\u00b0C<\/td>\n<\/tr>\n<tr>\n<td><strong>Shipping Conditions<\/strong><\/td>\n<td>Gel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4e19\u916e\u9178\u6fc0\u9176\u6d3b\u6027\u6bd4\u8272\/\u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fK709 Pyruvate Kinase Activity Col [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[467],"tags":[2519],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18058"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18058"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18058\/revisions"}],"predecessor-version":[{"id":18059,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18058\/revisions\/18059"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18058"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18058"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18058"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}