{"id":18009,"date":"2018-10-02T13:42:23","date_gmt":"2018-10-02T05:42:23","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=18009"},"modified":"2023-03-17T16:46:21","modified_gmt":"2023-03-17T08:46:21","slug":"%e9%81%8e%e6%b0%a7%e5%8c%96%e7%89%a9%e9%85%b6%e6%b4%bb%e6%80%a7%e6%aa%a2%e6%b8%ac-peroxidase-activity-colorimetricfluorometric-assay-kit%e8%b2%a8%e8%99%9f7012-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=18009","title":{"rendered":"POD\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6aa2\u6e2c | Peroxidase Activity Assay Kit\u8ca8\u865fK772-100 (Abcam ab155895)"},"content":{"rendered":"

POD\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6bd4\u8272\/\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865fK772-100\u00a0\u00a0(Abcam ab155895)<\/strong><\/span><\/a><\/span><\/p>\n

Peroxidase Activity Colorimetric\/Fluorometric Assay Kit<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1aPeroxidases<\/strong>\u904e\u6c27\u5316\u7269\u9176\uff08EC\u7de8\u865f1.11.1.x\uff09\u662f\u4e00\u5927\u985e\u9176\uff0c\u5176\u901a\u5e38\u50ac\u5316\u5f62\u5f0f\u7684\u53cd\u61c9\uff1aROOR’+\u96fb\u5b50\u4f9b\u9ad4\uff082e-\uff09+ 2H +\u2192ROH + R’OH\u3002\u5c0d\u65bc\u8a31\u591a\u9019\u4e9b\u9176\uff0c\u6700\u4f73\u5e95\u7269\u662fhydrogen peroxide\u904e\u6c27\u5316\u6c2b\uff0c\u4f46\u662f\u5176\u4ed6\u9176\u5c0dorganic hydroperoxides\u6709\u6a5f\u6c2b\u904e\u6c27\u5316\u7269\u5982lipid peroxides\u8102\u8cea\u904e\u6c27\u5316\u7269\u66f4\u5177\u6d3b\u6027\u3002Peroxidases\u904e\u6c27\u5316\u7269\u9176\u53ef\u5728\u5176\u6d3b\u6027\u4f4d\u9ede\u542b\u6709heme cofactor\u8840\u7d05\u7d20\u8f14\u56e0\u5b50\uff0c\u6216\u8005\u53ef\u9078\u5730\u542b\u6709\u6c27\u5316\u9084\u539f\u6d3b\u6027\u7684cysteine\u534a\u80f1\u6c28\u9178\u6216selenocysteine residues\u7852\u4ee3\u534a\u80f1\u6c28\u9178\u6b98\u57fa\u3002 BioVision\u7684Peroxidase Assay Kit\u904e\u6c27\u5316\u7269\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u65b9\u4fbf\u7684\u6bd4\u8272\u548c\u87a2\u5149\u6e2c\u5b9a\u65b9\u6cd5\u4f86\u6e2c\u91cf\u751f\u7269\u6a23\u54c1\u4e2d\u7684\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u3002\u5728\u904e\u6c27\u5316\u7269\u9176\u5b58\u5728\u4e0b\uff0cOxiRed\u63a2\u91dd\u4ee51\uff1a1\u7684\u5316\u5b78\u8a08\u91cf\u8207H2O2\u53cd\u61c9\uff0c\u7522\u751f\u7d05\u8272\u87a2\u5149\u6c27\u5316\u7522\u7269-resorufin\u8a66\u9e75\u9748\u3002\u901a\u904e\u6bd4\u8272\uff08lmax = 570nm\uff09\u6216\u87a2\u5149\u6e2c\u5b9a\u6cd5\uff08Ex \/ Em = 535 \/ 587nm\uff09\u91cf\u5316, resorufin\u3002\u8a72\u6e2c\u5b9a\u6cd5\u7c21\u55ae\uff0c\u76f4\u63a5\uff0c\u9ad8\u9748\u654f\u5ea6\u548c\u9ad8\u901a\u91cf\u6e96\u5099\u3002\u6839\u64da\u6211\u5011\u7684\u55ae\u4f4d\u5b9a\u7fa9\uff0c\u901a\u904e\u6bd4\u8272\u6cd5\u6aa2\u6e2c\u9650\u70ba\u6bcf\u5b540.1 mU\u6216\u901a\u904e\u87a2\u5149\u6cd5\u6aa2\u6e2c\u6bcf\u5b540.01 mU\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK772-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (570 nm) or Fluorescence (Ex\/Em=535\/587)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe kit has a detection limit of 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 The assay is simple, direct, highly sensitive and high-throughput adaptable<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Assay Buffer
\n\u2022 OxiRed\u2122 Probe (in DMSO)
\n\u2022 H\u2082O\u2082 Substrate (0.88 M)
\n\u2022 HRP Positive Control<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

POD\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6bd4\u8272\/\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK772-100\u00a0\u00a0(Abcam ab155895) Per […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[2529],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18009"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18009"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18009\/revisions"}],"predecessor-version":[{"id":48865,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/18009\/revisions\/48865"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18009"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18009"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18009"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}