{"id":17962,"date":"2018-09-25T17:30:06","date_gmt":"2018-09-25T09:30:06","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17962"},"modified":"2022-07-20T15:38:00","modified_gmt":"2022-07-20T07:38:00","slug":"potato-dextrose-agar%e9%a6%ac%e9%88%b4%e8%96%af%e8%91%a1%e8%90%84%e7%b3%96%e7%93%8a%e8%84%82-yeasts%e9%85%b5%e6%af%8d%e5%92%8cmoulds%e9%bb%b4%e8%8f%8c%e8%a8%88%e7%ae%97%e4%b9%8b%e5%9f%b9%e9%a4%8a","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=17962","title":{"rendered":"Potato Dextrose Agar\u99ac\u9234\u85af\u8461\u8404\u7cd6\u74ca\u8102| yeasts\u9175\u6bcd\u548cmoulds\u9ef4\u83cc\u8a08\u7b97\u4e4b\u57f9\u990a\u57fa \u8ca8\u865f LAB098-A"},"content":{"rendered":"

Potato Dextrose Agar\u99ac\u9234\u85af\u8461\u8404\u7cd6\u74ca\u8102| <\/strong>\u8ca8\u865f<\/strong> LAB098-A<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Potato Dextrose Agar\u99ac\u9234\u85af\u8461\u8404\u7cd6\u74ca\u8102\uff0c\u88abAmerican Public Health Association\u7f8e\u570b\u516c\u5171\u885b\u751f\u5354\u6703\u63a8\u85a6\uff0c\u7528\u65bc\u6aa2\u67e5\u4e73\u88fd\u54c1\uff0c\u8edf\u98f2\u6599\uff0c\u4e7e\u71e5\u548c\u51b7\u51cd\u98df\u54c1\u4ee5\u53ca\u5176\u4ed6\u985e\u578b\u7522\u54c1\u4e2d, yeasts\u9175\u6bcd\u548cmoulds\u9ef4\u83cc\u8a08\u7b97\u4e4b\u57f9\u990a\u57fa\u3002 \u53d6\u6c7a\u65bcmedium\u662f\u5426\u662f\u7528\u4f5c\u9078\u64c7\u6027\u6216\u975e\u9078\u64c7\u6027\u74ca\u8102\uff0c\u53ef\u8207\u9178\u5316\u6216\u4e0d\u9178\u5316\u4e00\u8d77\u4f7f\u7528\u3002<\/p>\n

\"\"<\/p>\n

\u57f9\u990a\u57fa\u4f7f\u7528\u65b9\u6cd5<\/strong>: <\/strong>\u7a31\u53d639\u514b\u7c89\u672b\uff0c\u5206\u6563\u57281\u5347\u53bb\u96e2\u5b50\u6c34\u4e2d\uff0c\u7136\u5f8c\u5728121\u2103\u4e0b\u6ec5\u83cc15\u5206\u9418\u3002 \u5728\u5012\u5165\u7121\u83cc\u4e4b\u524d\u5145\u5206\u6df7\u5408\u57f9\u990a\u76bf\u3002 \u5728\u67d0\u4e9b\u60c5\u6cc1\u4e0b\uff0c\u53ef\u80fd\u9700\u8981\u5c07\u57f9\u990a\u57fa\u7684pH\u964d\u4f4e\u81f33.5\u4ee5\u6291\u5236\u7d30\u83cc\u751f\u9577\u3002 \u9019\u53ef\u4ee5\u901a\u904e\u54111\u5347\u99ac\u9234\u85af\u8461\u8404\u7cd6\u74ca\u8102LAB 98\u4e2d\u52a0\u516510ml\u7121\u83cc10\uff05\u4e73\u9178X037\u4f86\u5b8c\u6210\u3002\u9ad8\u58d3\u6ec5\u83cc\u4e26\u51b7\u537b\u81f347\u00b0C\u3002 \u4e00\u65e6pH\u964d\u4f4e\uff0c\u5247\u53ef\u4ee5\u4e0d\u518d\u52a0\u71b1\u4ecb\u8cea\uff0c\u4e14\u4e0d\u6703\u5c0e\u81f4\u7531\u74ca\u8102\u6c34\u89e3\u5f15\u8d77\u4e4b\u51dd\u81a0\u5f37\u5ea6\u640d\u5931\u60c5\u6cc1\u3002<\/p>\n

\"\u672a\u547d\u540d-14\"<\/a>\u00a0\u00a0Plate count agar<\/strong>\uff0c<\/strong>PCA<\/strong>\u57f9\u990a\u76bf\u8a08\u6578\u57f9\u990a\u57fa<\/strong>\\<\/strong>\u7e3d\u751f\u83cc\u6578\u57f9\u990a\u57fa<\/strong>\u00a0<\/span>|\u00a0<\/strong>\u8ca8\u865f<\/strong>LAB149-A<\/strong><\/a><\/span><\/p>\n

\u570b\u969b\u6a19\u6e96PCA\uff0c\u7528\u65bc\u7e3d\u7d30\u83cc\u4e2d\u7684\u6e2c\u5b9a\uff08GB2008\u3001SN\u6a19\u6e96\uff09\u3002\u5546\u54c1\u5316\u7d30\u83cc\u7528\u57f9\u990a\u57faPCA\uff1a\u4e3b\u8981\u7528\u65bc\u7d30\u83cc\u57f9\u990a\uff1b\u6216\u4ea6\u53ef\u9078\u53d6\u80f0\u86cb\u767d\u5927\u8c46\u74ca\u8102\u57f9\u990a\u57fa\uff08Tryptone soy agar\uff0cTSA\uff09\u4e8c\u8005\u4e4b\u4e00\u57f9\u990a\u7d30\u83cc\u3002<\/p>\n

\"LAB149\"<\/p>\n

LAB M\u00a0\u00a0<\/strong>\u7c89\u672b\u57f9\u990a\u57fa | \u82f1\u570b\u539f\u88dd\u9032\u53e3<\/strong><\/p>\n

\"\u672a\u547d\u540d-14\"<\/a>\u00a0Harlequin\u2122<\/span>\u5448\u8272\u57f9\u990a\u57fa\u7cfb\u5217\u5546\u54c1<\/p>\n

LAB M\u4f4d\u65bc\u82f1\u570b\uff0c\u662f\u57f9\u990a\u57fa\u8001\u54c1\u724c\u516c\u53f8\uff0c\u53e3\u7891\u597d\u54c1\u8cea\u4f73\uff0c\u63a1\u7528\u5bc6\u5c01\u5c01\u53e3\u819c\uff0c\u539f\u88dd\u9032\u53e3\u57f9\u990a\u57fa\u4e0d\u6613\u53d7\u6f6e\uff0c\u4ea6\u6c92\u6709\u5206\u88dd\u9810\u6df7\u4e4b\u7591\u616e\u3002Harlequin\u2122<\/span>\u5448\u8272\u57f9\u990a\u57fa\u7cfb\u5217\u5546\u54c1\u8207\u5404\u5f0f\u57f9\u990a\u57fa\u6dfb\u52a0\u7269\uff0c\u63d0\u4f9b\u5feb\u901f\u548c\u6e96\u78ba\u7684\u65b9\u6cd5\u5206\u96e2\u8207\u5224\u8b80\u76ee\u6a19\u5fae\u751f\u7269\u3002\u96d9\u8272\u5224\u8b80\uff0c\u7c21\u6613\u5feb\u901f\uff1b\u5728\u98df\u54c1\u5b89\u5168\u53ca\u81e8\u5e8a\u8a3a\u65b7\u7686\u7b26\u5408\u76f8\u95dc\u898f\u7bc4\uff08ISO 9001:2000\u548cISO13485\uff0c\u4ee5\u53ca\u6b50\u6d32IVD\u6307\u4ee4CE\u6a19\u8a8c\uff09\u3002<\/p>\n

\"HAL008\"\"PIN001-web\"\"PIN005-web\"<\/p>\n

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 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

\u5206\u751f\u5c08\u7528\u57f9\u990a\u57faLB Broth\u8207LB Agar\uff0c\u65b9\u4fbf\u9032\u884cDNA Cloning\u3001Yeast\u57f9\u990a\u7b49\u5be6\u9a57\uff0c\u662f E. Coli \u8868\u73fe\u7cfb\u7d71\u6700\u4f73\u7684\u9078\u64c7\uff01<\/p>\n

\"\u672a\u547d\u540d-14\"<\/a>\u00a0Tryptone\u00a0<\/b>\u916a\u86cb\u767d<\/strong>(casein)<\/strong>\u6c34\u89e3\u7269<\/strong>\u00a0|\u00a0<\/strong>\u8ca8\u865f<\/strong>MC005-A<\/strong><\/p>\n

Tryptone\u5e38\u898b\u65bc\u5fae\u751f\u7269\u57f9\u990a\u8207\u767c\u9175\u4f7f\u7528\uff0c\u4e5f\u662f\u7f8e\u570b\u85e5\u5178\u898f\u7bc4\u7684\u6a19\u6e96\u57f9\u990a\u57fa\u6210\u5206\u4e4b\u3127\u3002Tryptone\u662f\u916a\u86cb\u767d(casein)\u7d93\u7531\u80f0\u81df\u9175\u7d20\u6c34\u89e3(pancreatic digest)\u800c\u6210\u7684\u71df\u990a\u6210\u5206\uff0c\u4e5f\u662f\u4e00\u822c\u6240\u8b02\u80f0\u6c34\u89e3\u86cb\u767d\u7684\u901a\u7528\u540d\u7a31\u3002\u916a\u86cb\u767d\u662f\u725b\u5976\u4e2d\u4e3b\u8981\u7684\u86cb\u767d\u8cea\u6210\u5206\uff0c\u5bcc\u542b\u5404\u7a2e\u80fa\u57fa\u9178\u6c2e\u6e90\uff0c\u5c24\u5176\u662f\u8272\u80fa\u9178(tryptophan)\u6bd4\u4f8b\u76f8\u7576\u9ad8\u3002Tryptone\u7684\u7528\u9014\u5f88\u5ee3\uff0c\u662fLB medium\u3001Terrific broth\u3001Tryptic soy medium (TSB)\u8207Fluid Thioglycollate Medium (FTM)\u7b49\u5e38\u7528\u57f9\u990a\u57fa\u7684\u4e3b\u8981\u6210\u5206\uff0c\u4e5f\u662f\u6297\u751f\u7d20\u3001\u80de\u5916\u86cb\u767d\u8cea(extra-cellular protein)\u3001\u5e72\u64fe\u7d20(interferon)\u8207\u75ab\u82d7\u6bd2\u7d20\u7b49\u767c\u9175\u88fd\u7a0b\u4e2d\u91cd\u8981\u7684\u57f9\u990a\u539f\u6599\u3002<\/p>\n

\"\"<\/p>\n

\"\u672a\u547d\u540d-14\"<\/a>\u00a0Yeast extract |\u00a0<\/strong>\u9175\u6bcd\u8403\u53d6\u7269<\/strong>(yeast extract) |<\/strong>\u8ca8\u865f<\/strong>MC001-A<\/strong><\/p>\n

\u53ef\u4ee5\u63d0\u9ad8 E. Coli DH5\u03b1 \u5bc6\u5ea6\u9054\u50b3\u7d71 LB broth \u3002Yeast extract \u63d0\u4f9b\u4e86\u8c50\u5bcc\u7684\u71df\u990a\u6210\u5206\uff0cSaccharomyces\u9175\u6bcd\u7d30\u80de\u7d93\u904e\u81ea\u52d5\u5206\u89e3(autolysate)\u5f8c\uff0c\u9664\u4e86\u5bcc\u542b\u5404\u5f0f\u5fae\u751f\u7269\u4ee5\u53ca\u52d5\u7269\u7d30\u80de\u751f\u9577\u6240\u9700\u7684\u80fa\u57fa\u9178\u6c2e\u6e90\u5916\uff0c\u9084\u6709\u542b\u91cf\u6975\u9ad8\u7684\u53ef\u6eb6\u6027\u7dad\u751f\u7d20B\u7fa4\u3002\u5305\u62ec vitamins\u3001amino acids\u3001peptides \u8207 carbohydrates \u7b49\u3002\u5176\u9ad8\u71df\u990a\u50f9\u503c\u4e5f\u662f\u80ce\u725b\u8840\u6e05 FBS \u6700\u4f73\u66ff\u4ee3\u7269\uff0c\u662f\u751f\u7269\u88fd\u7a0b\u4e2d\u6975\u5177\u91cd\u8981\u6027\u7684\u975e\u52d5\u7269\u6027\u86cb\u767d\u4e4b\u4f86\u6e90\u3002\u56e0\u6b64\u57f9\u990a\u57fa\u914d\u65b9\u3001\u5fae\u751f\u7269\u767c\u9175\u539f\u6599\u3001\u8840\u6e05\u66ff\u4ee3\u7269\u3001\u6297\u9ad4\u751f\u7522\u8207\u86cb\u767d\u8cea\u85e5\u7269\u7b49\u90fd\u63a1\u7528yeast extract\u4f5c\u70ba\u71df\u990a\u4f86\u6e90\u3002<\/p>\n

\"Yeast<\/p>\n

\u9ef4\u83cc\u53ca\u9175\u6bcd\u83cc\u57f9\u990a\uff0c\u4f7f\u7528\u6c99\u6c0f\u8461\u8404\u7cd6\u57f9\u990a\u57fa\uff08Sabouraud Dextrose Agar\uff0cSDA\uff09 \u6216\u99ac\u9234\u85af\u8461\u8404\u7cd6\u57f9\u990a\u57fa\uff08Potato dextrose agar\uff0cPDA\uff09\u3002Potato Dextrose Agar \u00a0| \u99ac\u9234\u85af\u8461\u8404\u7cd6\u6d0b\u83dc\u57f9\u990a\u57fa |\u8ca8\u865fLAB098-A<\/p>\n

\"PD30_jp\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
LAB004-A<\/td>\nTryptone Soy Broth USP (Soybean Casein Digest Medium)<\/td>\n500g<\/td>\n<\/tr>\n
LAB011-A<\/td>\nTryptone Soy Agar USP (Soybean Casein Digest Medium)<\/td>\n500g<\/td>\n<\/tr>\n
LAB028-A<\/td>\nBlood Agar Base<\/td>\n500g<\/td>\n<\/tr>\n
LAB061-A<\/td>\nEosin Methylene Blue Agar (Levine)<\/td>\n500g<\/td>\n<\/tr>\n
LAB093-A<\/td>\nMRS Agar<\/td>\n500g<\/td>\n<\/tr>\n
LAB094-A<\/td>\nMRS Broth<\/td>\n500g<\/td>\n<\/tr>\n
LAB098-A<\/td>\nPotato Dextrose Agar<\/td>\n500g<\/td>\n<\/tr>\n
LAB108-A<\/td>\nPseudomonas Agar Base<\/td>\n500g<\/td>\n<\/tr>\n
LAB149-A<\/td>\nPlate Count Agar<\/td>\n500g<\/td>\n<\/tr>\n
LAB168-A<\/td>\nL.B. Agar<\/td>\n500g<\/td>\n<\/tr>\n
LAB169-A<\/td>\nLB Broth<\/td>\n500g<\/td>\n<\/tr>\n
LAB196-A<\/td>\nLauryl Tryptose Broth<\/td>\n500g<\/td>\n<\/tr>\n
MC001-A<\/td>\nYeast Extract Powder<\/td>\n500g<\/td>\n<\/tr>\n
MC003-A<\/td>\nSoy Peptone<\/td>\n500g<\/td>\n<\/tr>\n
MC005-A<\/td>\nTryptone<\/td>\n500g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u66f4\u591a\u76f8\u95dc\u5546\u54c1<\/span>\u00a0\u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a>| Agarose |\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a>\u00a0|\u00a0\u5fae\u751f\u7269\u6297\u751f\u7d20<\/a>\u00a0|\u00a0\u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a>\u00a0|Mupid 2 DNA\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a>|\u00a03M\u5fae\u751f\u7269\u5feb\u901f\u6aa2\u6e2c\u7247<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Potato Dextrose Agar\u99ac\u9234\u85af\u8461\u8404\u7cd6\u74ca\u8102| \u8ca8\u865f LAB098-A \u7522\u54c1\u4ecb\u7d39: Potato […]<\/p>\n","protected":false},"author":3,"featured_media":17967,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[475,1],"tags":[2539],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17962"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17962"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17962\/revisions"}],"predecessor-version":[{"id":17968,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17962\/revisions\/17968"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/17967"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17962"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17962"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17962"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}