{"id":17945,"date":"2018-09-20T17:05:05","date_gmt":"2018-09-20T09:05:05","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17945"},"modified":"2022-07-20T15:45:05","modified_gmt":"2022-07-20T07:45:05","slug":"genecraft-r-crispr-sgrna-screening-kit-s-pyogenes-%e7%af%a9%e9%81%b8%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9f-g953","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=17945","title":{"rendered":"GeneCraft-R CRISPR sgRNA Screening Kit (S. pyogenes) \u7be9\u9078\u8a66\u5291\u76d2 | \u8ca8\u865f   G953"},"content":{"rendered":"<p><strong>CRISPR sgRNA<\/strong><strong>\u7be9\u9078 <\/strong><\/p>\n<p><strong>\u00a0<\/strong>abm\u7684GeneCraft-R CRISPR sgRNA\u7be9\u9078\u8a66\u5291\u76d2\uff08S.pyogenes\uff09\u662f\u4e00\u7a2e\u65b9\u4fbf\u6709\u6548\u7684\u5de5\u5177\uff0c\u53ef\u7528\u65bc\u9078\u64c7\u6700\u6709\u524d\u9014\u7684sgRNA\u8a2d\u8a08\uff0c\u7528\u65bcspCas9\u7684\u57fa\u56e0\u7d44\u7de8\u8f2f\u3002<\/p>\n<p><strong>\u00a0<\/strong><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=17945\"><strong>GeneCraft-R CRISPR sgRNA Screening Kit (S. pyogenes) <\/strong><strong>\u7be9\u9078\u8a66\u5291\u76d2 | <\/strong><strong>\u8ca8\u865f<\/strong><strong>\u00a0\u00a0 <\/strong><strong>G953<\/strong><\/a><\/span><\/p>\n<p>\u7522\u54c1\u4ecb\u7d39: \u7be9\u9078sgRNA\u5728\u9ad4\u5916\u91dd\u5c0dPCR\u7522\u751f\u7684gDNA\u6a21\u677f\u7684\u5207\u5272\u6548\u7387\u3002 \u53ef\u4ee5\u4f7f\u7528GeneCraft-R Express\u6216Classic CRISPR sgRNA\u5408\u6210\u8a66\u5291\u76d2\uff08\u5206\u5225\u70ba\u76ee\u9304\u865fG948\u548cG952\uff09\u5408\u6210sgRNA\u3002 \u7be9\u9078\u7684\u76ee\u6a19\u6a21\u677f\u4f7f\u7528\u8a72\u8a66\u5291\u76d2\u4e2d2X PCR Bestaq\u2122MasterMix\u9032\u884cPCR\u751f\u6210\u3002 sgRNA\u7be9\u9078\u689d\u4ef6\u5df2\u7d93\u512a\u5316\uff0csgRNA\u5207\u5272\u53cd\u61c9\u53ef\u57281\u5c0f\u6642\u5167\u5b8c\u6210\u3002 \u9ad4\u5916\u7be9\u9009\u548c\u9078\u64c7\u6700\u6709\u6548\u7684sgRNA\u8a2d\u8a08\u53ef\u4ee5\u5927\u5927\u6e1b\u5c11\u4efb\u4f55\u4e0b\u6e38CRISPR\u57fa\u56e0\u7d44\u7de8\u8f2f\u61c9\u7528\u7684\u6642\u9593\u548c\u52de\u52d5\u529b\u6295\u5165\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/Enzymes\/images\/GeneCraft-R-screening-07v2-07-07.jpg\" alt=\"\" width=\"583\" height=\"1020\" \/><\/p>\n<p>Store all components at -20\u00b0C.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CRISPR sgRNA\u7be9\u9078 \u00a0abm\u7684GeneCraft-R CRISPR sgRNA\u7be9\u9078\u8a66\u5291\u76d2\uff08S.pyo [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":17943,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[88],"tags":[2543],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17945"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17945"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17945\/revisions"}],"predecessor-version":[{"id":17946,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17945\/revisions\/17946"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/17943"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17945"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17945"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17945"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}