{"id":17928,"date":"2018-09-19T16:34:46","date_gmt":"2018-09-19T08:34:46","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17928"},"modified":"2025-03-21T14:46:01","modified_gmt":"2025-03-21T06:46:01","slug":"%e6%b0%b8%e7%94%9f%e5%8c%96%e7%9a%84%e5%b0%8f%e9%bc%a0%e8%bf%91%e7%ab%af%e5%b0%8f%e7%ae%a1kpt11%e7%b4%b0%e8%83%9e-immortalized-mouse-proximal-tubule-kpt11-cells-%e8%b2%a8%e8%99%9ft0640","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=17928","title":{"rendered":"\u6c38\u751f\u5316\u7684\u5c0f\u9f20\u8fd1\u7aef\u5c0f\u7ba1KPT11\u7d30\u80de | Immortalized Mouse Proximal Tubule KPT11 Cells \u8ca8\u865fT0640"},"content":{"rendered":"

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong><\/p>\n

\u6c38\u751f\u5316\u7684\u5c0f\u9f20\u8fd1\u7aef\u5c0f\u7ba1KPT11\u7d30\u80de\u00a0 |\u00a0\u8ca8\u865f<\/strong>T0640<\/a><\/span><\/p>\n

Immortalized Mouse Proximal Tubule KPT11 Cells<\/p>\n

\u6c38\u751f\u5316\u7684\u5c0f\u9f20\u8fd1\u7aef\u5c0f\u7ba1KPT11\u7d30\u80de\u662f\u4f86\u81ea\u9f20\u814e\u7684\u81ea\u767c\u6c38\u751f\u7d30\u80de\u3002 KPT11\u7d30\u80de\u5c0dgentamicin\u6176\u5927\u9709\u7d20\u8655\u7406\u66f4\u654f\u611f\uff0c\u56e0\u70ba\u8207\u6c38\u751f\u5316\u5c0f\u9f20\u9060\u7aef\u5c0f\u7ba1KDT3\u7d30\u80de\uff08abm\uff0cT0641\uff09\u76f8\u6bd4\uff0c\u89c0\u5bdf\u5230gentamicin\u6176\u5927\u9709\u7d20\u8a98\u5c0e\u7684\u6bd2\u6027\u3002 \u767c\u73feKPT11\u7d30\u80de\u5177\u6709\u66f4\u9ad8\u5bc6\u5ea6\u7684gentamicin\u6176\u5927\u9709\u7d20\u7d50\u5408\u86cb\u767d\uff0c\u4f8b\u5982CLIMP-63\u3002 \u8a72\u7d30\u80de\u4fc2\u662f\u7528\u65bc\u7814\u7a76gentamicin\u6176\u5927\u9709\u7d20\u7d50\u5408\u86cb\u767d\u7684\u6a5f\u5236\u548c\u6176\u5927\u9709\u7d20\u5728\u7d30\u80de\u51cb\u4ea1\u4e2d\u7684\u4f5c\u7528\u7684\u6709\u50f9\u503c\u7684\u7d30\u80de\u5de5\u5177\u3002\"\"<\/p>\n

\u6c38\u751f\u5316\u7684\u5c0f\u9f20\u8fd1\u7aef\u5c0f\u7ba1KPT11\u7d30\u80de<\/strong>\u3002\u7d30\u80de\u7279\u6027: Adherent \/ Morphology \u00a0Fibroblast-like | \u4e0d\u6747\u65b9\u6cd5: Spontaneously immortalization | \u4f86\u6e90Kidney\u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a><\/span>)\uff0c\u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a><\/span>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrow VI (TM016) \u3002\u70ba\u4e86\u88fd\u5099\u5b8c\u6574\u7684\u751f\u9577\u57f9\u990a\u57fa\uff0c\u5c07\u4e0b\u5217\u7d44\u5206\u6dfb\u52a0\u5230\u57fa\u790e\u57f9\u990a\u57fa\u4e2d\uff1a\u80ce\u725b\u8840\u6e05\uff08TM999\uff09\u81f3\u6700\u7d42\u6fc3\u5ea610%\uff0cPenicillin\/Streptomycin\u9752\u9ef4\u7d20\/\u93c8\u9ef4\u7d20\u6eb6\u6db2\uff08G255\uff09\u81f3\u6700\u7d42\u6fc3\u5ea6\u70ba1\uff05\u3002\u4e8c\u6c27\u5316\u78b3\uff08CO2\uff09\uff1a5\uff05\uff0c\u6eab\u5ea6\uff1a37.0\u2103\"\"<\/p>\n

\u7522\u54c1\u8cc7\u6599\u8acb\u6309\u9019PriCoat\u2122 T25 Flasks | type I collagen coating | <\/a>I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix | <\/a>Prigrow III Medium\u521d\u4ee3\u7d30\u80de\u57f9\u990a\u6db2 <\/a><\/span><\/p>\n

HOT!!<\/strong>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

Abmgood<\/strong>\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized reagenet)<\/strong>\u00a0Ready-to-use<\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat. No.<\/td>\nT0640<\/span><\/td>\n<\/tr>\n
Name<\/td>\nImmortalized Mouse Proximal Tubule KPT11 Cells<\/td>\n<\/tr>\n
Description<\/td>\nImmortalized Mouse Proximal Tubule KPT11 Cells are spontaneously immortal derived cells from murine kidney. KPT11 cells are more susceptible to gentamicin treatments, as gentamicin-induced toxicity was observed compared to the Immortalized Mouse Distal Tubule KDT3 Cells (Cat. No.\u00a0T0641<\/a>). It is found that the KPT11 cells have a higher density of gentamicin-binding proteins, such as CLIMP-63. This cell line is a valuable tool for studies relating to the mechanisms of the gentamicin-binding proteins and effects of gentamicin in apoptosis.<\/td>\n<\/tr>\n
Organism<\/td>\nMouse (M. musculus)<\/td>\n<\/tr>\n
Tissue<\/td>\nKidney<\/td>\n<\/tr>\n
Growth Properties<\/td>\nAdherent, fibroblast-like<\/td>\n<\/tr>\n
Cell Type<\/td>\nImmortalized Cells<\/td>\n<\/tr>\n
Storage Condition<\/td>\nVapor phase of liquid nitrogen, or below -130\u00b0C.<\/td>\n<\/tr>\n
Shipping Conditions<\/td>\nShip with dry ice.<\/td>\n<\/tr>\n
Product Format<\/td>\nFrozen<\/td>\n<\/tr>\n
Intended Use<\/td>\nThis product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.<\/td>\n<\/tr>\n
BioSafety<\/td>\nII<\/td>\n<\/tr>\n
Certificate of Analysis<\/td>\nFor batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com.<\/td>\n<\/tr>\n
Growth Conditions<\/td>\nUse of PriCoat\u2122 T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow VI (TM016) + 10% FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0\u00b0C, 5% CO\u2082<\/td>\n<\/tr>\n
Unpacking and Storage Instructions<\/td>\n\n

1. Visually examine the packaging containers for signs of leakage or breakage.<\/p>\n

2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130\u00b0C, preferably in liquid nitrogen vapor phase storage, until ready for use.<\/p>\n

To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130\u00b0C or in liquid nitrogen vapor phase. Do not store at -70\u00b0C, as it will result in loss of viability.<\/td>\n<\/tr>\n

Thawing Protocol<\/td>\n\n

1. Thaw cells quickly in a 37\u00b0C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.<\/p>\n

2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.<\/p>\n

3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.<\/p>\n

4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.<\/p>\n

5. Incubate the cells at the recommended conditions.<\/td>\n<\/tr>\n

Subculture Protocol<\/td>\n\n

Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.<\/p>\n

1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.<\/p>\n

2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37\u00b0C, for several minutes to facilitate detachment.<\/p>\n

3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.<\/p>\n

4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.<\/p>\n

5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.<\/p>\n

6. Incubate the cells at the recommended conditions.<\/td>\n<\/tr>\n

Cryopreservation<\/td>\nCryopreservation Medium (TM024), or complete growth media with 10% DMSO.<\/td>\n<\/tr>\n
Seeding Density (cells\/cm2<\/sup>)<\/td>\n20,000 – 50,000<\/td>\n<\/tr>\n
Immortalization Method<\/td>\nSpontaneous immortalization<\/td>\n<\/tr>\n
Expression<\/td>\nCLIMP-63<\/td>\n<\/tr>\n
Material Citation<\/td>\nIf use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T0640.<\/td>\n<\/tr>\n
Warranty<\/td>\nabm<\/strong>\u00a0warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable\u00a0abm<\/strong>\u00a0Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the “Warranty Period\u201d.<\/td>\n<\/tr>\n
Disclaimer<\/td>\n\n
1. All test parameters provided in the CoA are conducted using\u00a0abm<\/strong>‘s standardized culture system and The stated values may vary under the end-user’s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 \u03bcg, Cat.# C207, $450.00) or cell lysate (100 \u03bcg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.<\/div>\n
2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of order placement. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination’s temperature variation and its geographical location.<\/div>\n
3. All of\u00a0abm<\/strong>‘s cell biology products are for research use ONLY and NOT for therapeutic\/diagnostic applications.\u00a0abm<\/strong>\u00a0is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic\/diagnostic or any other non-RUO application(s).<\/div>\n
4.\u00a0abm<\/strong>\u00a0makes no warranties or representations as to the accuracy of the information on this site. Citations from literature are provided for informational purposes only.\u00a0abm<\/strong>\u00a0does not warrant that such information has been shown to be accurate.<\/div>\n
5.\u00a0abm<\/strong>\u00a0warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable\u00a0abm<\/strong>\u00a0Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the “Warranty Period”.<\/div>\n<\/td>\n<\/tr>\n
Depositor<\/td>\nOregon Health & Science University<\/td>\n<\/tr>\n
Application<\/td>\nResearch Use Only.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1 (immortalized cell) \u6c38\u751f\u5316\u7684\u5c0f\u9f20\u8fd1\u7aef\u5c0f\u7ba1KPT11\u7d30\u80de\u00a0 | […]<\/p>\n","protected":false},"author":3,"featured_media":17929,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373,455],"tags":[2547],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17928"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17928"}],"version-history":[{"count":8,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17928\/revisions"}],"predecessor-version":[{"id":50826,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17928\/revisions\/50826"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/17929"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17928"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17928"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17928"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}