{"id":17899,"date":"2018-09-13T17:28:28","date_gmt":"2018-09-13T09:28:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17899"},"modified":"2022-07-20T15:59:01","modified_gmt":"2022-07-20T07:59:01","slug":"fndc5%e6%b4%bb%e6%80%a7%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-%e8%b2%a8%e8%99%9fapn576hu61","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=17899","title":{"rendered":"FNDC5\u6d3b\u6027\u91cd\u7d44\u86cb\u767d |\u8ca8\u865fAPN576Hu61"},"content":{"rendered":"

\u9644<\/em><\/strong>Protein marker <\/em><\/strong>\u6216<\/em><\/strong> positive control <\/em><\/strong><\/p>\n

Active Fibronectin Type III Domain Containing Protein 5 (FNDC5) <\/strong>\u6d3b\u6027<\/strong>\u91cd\u7d44\u86cb\u767d <\/strong>|<\/strong>\u8ca8\u865f<\/strong>APN576Hu61<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Cloud clone\u63d0\u4f9b\u539f\u6838\u53ca\u771f\u6838\u751f\u7269\u8868\u73fe\u4e4b\u91cd\u7d44\u86cb\u767d\u7cfb\u5217\u5546\u54c1\uff1b\u5176\u4e2dActive Fibronectin Type III Domain Containing Protein 5 (FNDC5) \u6d3b\u6027\u91cd\u7d44\u86cb\u767d\uff0c\u70ba\u6d3b\u6027\u86cb\u767d\u3002\u7d14\u5ea6> 97%\uff0c\u63d0\u4f9b\u51cd\u4e7e\u578b\u5f0f\u5305\u88dd\u3002\u53ef\u61c9\u7528\u65bcCell culture; Activity Assays.\u7b49\u4e0d\u540c\u7814\u7a76\u3002<\/p>\n

\u86cb\u767d\u8cea\u4fdd\u5b58\u578b\u5f0f<\/strong><\/p>\n

\u86cb\u767d\u8cea\u6eb6\u65bcPBS, pH7.6, containing 5% trehalose.\u70ba\u51b7\u51cd\u4e7e\u71e5\u5305\u88dd\u3002\u4f7f\u7528\u524dReconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg\/mL\u907f\u514dvortex. \u77ed\u671f\u4fdd\u5b58Store at 2-8\u00b0C for one month.\u5206\u88dd-80\u00b0C\u9577\u671f12 \u500b\u6708\u3002\u8acb\u907f\u514d\u53cd\u8986\u51b7\u51cd\u89e3\u51cd\u3002<\/p>\n

FNDC5<\/strong>\u6d3b\u6027\u91cd\u7d44\u86cb\u767d\u76f8\u95dc\u4e4b\u6297\u9ad4<\/strong> | <\/strong>\u86cb\u767d\u8cea<\/strong> \u7522\u54c1<\/strong><\/p>\n

\u00a0\"\"<\/strong><\/p>\n

The protein encoded by FNDC5 can be cleaved into Irisin, which is a myokine linked to exercise and lean body mass. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5,000 cells\/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10\u00b5L of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours in at 37oC.
\nCell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope was shown in Figure 1.<\/p>\n

\"\"<\/p>\n

Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of FNDC5 for 48h. The dose-effect curve of FNDC5 was shown in Figure 2. It was obvious that FNDC5 significantly decreased cell viability of MDA-MB-231 cells. The ED50 for this effect is typically 1.875 nM.<\/p>\n

USAGE<\/h3>\n

Reconstitute in PBS (pH7.6) to a concentration of 0.1-1.0 mg\/mL. Do not vortex.<\/p>\n

\"APN576Hu61.jpg\"<\/p>\n

\"Active<\/p>\n

Figure. Western Blot; Sample: Recombinant FNDC5, Human.<\/h3>\n

STORAGEAvoid repeated freeze\/thaw cycles. Store at 2-8\u00b0C for one month. Aliquot and store at -80\u00b0C for 12 months.<\/p>\n

STABILITY<\/h3>\n

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37\u00b0C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.<\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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