{"id":17485,"date":"2018-08-16T13:07:25","date_gmt":"2018-08-16T05:07:25","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17485"},"modified":"2022-07-20T16:11:04","modified_gmt":"2022-07-20T08:11:04","slug":"anti-mouse-lfc%e5%a4%9a%e6%a0%aa%e6%8a%97%e9%ab%94-exalpha-biologicals-inc-x1089p","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=17485","title":{"rendered":"anti Mouse LFC\u591a\u682a\u6297\u9ad4 | Exalpha Biologicals inc. X1089P"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=17485\">anti Mouse LFC\u591a\u682a\u6297\u9ad4 | X1089P<\/a><\/span><\/p>\n<p>Sheep anti Mouse LFC (Rho guanine nucleotide exchange factor 2, ARHGEF2)<\/p>\n<p><strong>\u7522\u54c1\u4ecb\u7d39:<\/strong> Lfc\u662f\u5728NIH 3T3\u7d30\u80de\u4e2d\u7684\u764c\u57fa\u56e0\u7be9\u9078\u4e2d\u767c\u73fe\u7684118kDa\u86cb\u767d\u8cea\u3002 Lfc\u662f\u8207Lbc\u76f8\u95dc\u7684\u4fe1\u865f\u50b3\u5c0e\u86cb\u767d\uff0c\u4e26\u4e14\u662fDbl\u86cb\u767d\u5bb6\u65cf\u7684\u4e00\u90e8\u5206\u3002 Lfc\u662f\u5c0fGTP\u9176\u7684Rho\u5bb6\u65cf\u7684\u9ce5\u560c\u5464 &#8211; \u6838\u82f7\u9178\u4ea4\u63db\u56e0\u5b50\uff08GEF\uff09\u3002 Lfc\u4e5f\u88ab\u8b49\u660e\u662f\u7d72\u6c28\u9178 &#8211; \u8607\u6c28\u9178\u6fc0\u9176JNK\u7684\u4e0a\u6e38\u3002 Lfc\u986f\u793a\u4ecb\u5c0eRac\u4fe1\u865f\u50b3\u5c0e\u9014\u5f91\u7684\u6fc0\u6d3b\u3002Exalpha \u63d0\u4f9bSheep anti Mouse LFC\u591a\u682a\u6297\u9ad4\uff0c\u5176\u514d\u75ab\u539f\u4f7f\u7528GST\u878d\u5408\u7684Lfc\u86cb\u767d(\u6c28\u57fa\u91782\u81f3447\u7684\u86cb\u767d\u8cea) \uff0c\u7d93\u7531\u786b\u9178\u92a8\u6c89\u6fb1\u7d14\u5316\u6297\u9ad4\u3002\u6297\u9ad4\u4fdd\u5b58\u65bc\u542b\u67090.08\uff05\u758a\u6c2e\u5316\u9209\u7684\u78f7\u9178\u9e7d\u7de9\u885d\u9e7d\u6c34\u6eb6\u6db2\u3002\u53ef\u61c9\u7528\u65bcWestern Blotting\uff0c\u7522\u54c1\u61c9\u5132\u5b58\u65bc-20\u00baC\u3002\u5206\u88dd\u5f8c\u907f\u514d\u53cd\u8986\u51b7\u51cd\u8207\u89e3\u51cd\u3002\u7522\u54c1\u61c9\u7528\u6e2c\u8a66\u5831\u544a: \u901a\u904e4\u03bcg\/ ml\u7684\u514d\u75ab\u6c89\u6fb1 &#8211; \u86cb\u767d\u8cea\u5370\u8de1\u6aa2\u6e2c\u5c0f\u9f20\u7d44\u7e54\u4e2d\u7684Lfc\u3002\u6aa2\u6e2c\u5c0d\u61c9\u65bc\u91ce\u751f\u578bLfc\u7684\u5c0f\u9f20\u7d44\u7e54\u63d0\u53d6\u7269\u4e2d\u7684118kDa\u689d\u5e36\u3002\u4e5f\u53ef\u7528\u65bc\u514d\u75ab\u87a2\u5149\u8a66\u9a57\u3002\u61c9\u901a\u904e\u9023\u7e8c\u7a00\u91cb\u4ee5\u8a55\u4f30\u6700\u4f73\u6fc3\u5ea6\u3002<\/p>\n<p>&nbsp;<\/p>\n<table width=\"600\">\n<tbody>\n<tr>\n<td>Isotype<\/td>\n<td>IgG<\/td>\n<\/tr>\n<tr>\n<td>Product Type<\/td>\n<td>Polyclonal Antibody<\/td>\n<\/tr>\n<tr>\n<td>Units<\/td>\n<td>250 \u00b5g<\/td>\n<\/tr>\n<tr>\n<td>Host<\/td>\n<td>Sheep<\/td>\n<\/tr>\n<tr>\n<td>Species reactivity<\/td>\n<td>Mouse<\/td>\n<\/tr>\n<tr>\n<td>Application<\/td>\n<td>Immunofluorescence<br \/>\nImmunoprecipitation<br \/>\nWestern Blot<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong><span class=\"text-node\">\u5f15\u7528\u6587\u737b References<\/span><\/strong><br \/>\n1. Glaven, J.A., et al. The Dbl-related protein, Lfc, localizes to microtubules and mediates the activation of Rac signaling pathways in cells. J. Biol. Chem. 1999, 274, 2279-2285 2. Glaven, J.A., et al. Lfc and Lsc oncoproteins represent two new guanine nucleotide exchange factors for the Rho GTP-binding protein. J. Biol. Chem. 1996, 271, 27374-27381 3. Whitehead, I., et al. Expression cloning of lfc, a novel oncogene with structural similarities to guanine nucleotide exchange factors and to the regulatory region of protein kinase C. J. Biol. Chem. 1995, 270, 18388-18395<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>anti Mouse LFC\u591a\u682a\u6297\u9ad4 | X1089P Sheep anti Mouse LFC (Rho g [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":17345,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[459],"tags":[2618],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17485"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17485"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17485\/revisions"}],"predecessor-version":[{"id":17487,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17485\/revisions\/17487"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/17345"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17485"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17485"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17485"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}