{"id":17326,"date":"2018-07-23T14:24:53","date_gmt":"2018-07-23T06:24:53","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17326"},"modified":"2022-07-20T16:25:50","modified_gmt":"2022-07-20T08:25:50","slug":"uncoated-%e7%99%bd%e7%b4%b0%e8%83%9e%e4%bb%8b%e7%b4%a04%ef%bc%88il4%ef%bc%89elisa%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fksa077mu01","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=17326","title":{"rendered":"(Uncoated) \u767d\u7d30\u80de\u4ecb\u7d204\uff08IL4\uff09ELISA\u8a66\u5291\u76d2 |\u8ca8\u865fKSA077Mu01"},"content":{"rendered":"

Uncoated <\/strong>\u591a\u76e4\u5f0f<\/strong>ELISA<\/strong>\u5546\u54c1\u5168\u65b0\u4e0a\u5e02<\/strong>!!<\/strong><\/p>\n

(Uncoated) <\/strong>\u767d\u7d30\u80de\u4ecb\u7d204\uff08IL4\uff09ELISA\u8a66\u5291\u76d2 <\/strong>|<\/strong>\u8ca8\u865f<\/strong>KSA077Mu01<\/a><\/span><\/p>\n

ELISA Kit DIY Materials for Interleukin 4 (IL4)<\/p>\n

\u5546\u54c1: (Uncoated) \u767d\u7d30\u80de\u4ecb\u7d204\uff08IL4\uff09<\/strong>\u6aa2\u6e2c\u8a66\u5291\u76d2(<\/u>\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) <\/u>| Double-antibody Sandwich ELISA\u8ca8\u865fKSA077Mu01\uff0c\u61c9\u7528\u7269\u7a2e\u70baMus musculus (Mouse)\uff1b\u5075\u6e2c\u7684\u9748\u654f\u5ea67.8-500pg\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea63.3pg\/mL, \u53ef\u61c9\u7528\u65bcSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u4e4b\u6a23\u54c1\u4f86\u6e90\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002Cloud-Clone corp\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u4e4bELISA (\u9175\u7d20\u514d\u75ab\u5206\u6790\u5957\u7d44\/\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)\uff0c\u5c08\u9580\u751f\u7522\u7814\u7a76\u7528\u6aa2\u6e2c\u8a66\u5291\u7684\u751f\u6280\u516c\u53f8\uff0c\u65bc\u7f8e\u570b\u7814\u767c\u548c\u751f\u7522\uff0c\u7522\u54c1\u7dda\u5305\u62ecELISA Kit\u3001CLIA Kit\u3001Antibody\u53carecombinant protein\uff1bELISA\u7522\u54c1\u7cfb\u5217\u8d85\u904e6000\u7a2e\u4ee5\u4e0a\uff0c\u7269\u7a2e\u9f4a\u5168\u5305\u62ecrat\u3001mouse\u3001rabbit\u3001cavia\u3001human\u3001porcine\u3001bovine\u3001simian\u7b49\u591a\u7269\u7a2e\u5546\u54c1\u3002\u54c1\u8cea\u4fdd\u8b49\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3001\u9ad8\u9748\u654f\u5ea6\u8a2d\u8a08ELISA\u3002Uncoated<\/u><\/strong> ELISA<\/u><\/strong>\u578b\u5f0f<\/u><\/strong>\uff1b\u5305\u62ecCapture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate\u3002Buffer<\/a>\u7684\u90e8\u4efd\u53e6\u5916\u8cfc\u8cb7\uff0c\u5176\u6bcf\u7d44\u53ef\u9032\u884c10\u76e4ELISA\u5206\u6790\u3002<\/p>\n

(Uncoated) <\/strong>\u767d\u7d30\u80de\u4ecb\u7d204\uff08IL4\uff09<\/strong>\u6aa2\u6e2c\u8a66\u5291\u76d2 (\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)<\/strong>\u5957\u7d44\u5167\u5bb9<\/strong><\/p>\n

\u5305\u62ecCapture Antibody, Detection Antibody\u3001Standard\u3001Streptavidin-HRP\u3001TMB Substrate, 96-well Plate\u3002Buffer<\/a>\u7684\u90e8\u4efd\u53e6\u5916\u8cfc\u8cb7\uff0c\u82e5\u70baSandwich ELISA\u8cc7\u6599\u8acb\u6309\u6b64Assay Kit DIY Support Pack 1<\/a>\uff0ccompetitive method ELISA\u8cc7\u6599\u8acb\u6309\u6b64Assay Kit DIY Support Pack 2<\/a>\u5176\u6bcf\u7d44\u53ef\u9032\u884c10\u76e4ELISA\u5206\u6790\u3002<\/p>\n

\u767d\u7d30\u80de\u4ecb\u7d204\uff08IL4\uff09<\/strong>\u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong>\u64cd\u4f5c\u7c21\u55ae\u6d41\u7a0b<\/strong><\/p>\n

    \n
  1. Coat the plates with 100\u03bcL per well of working solution of Capture Antibody.incubate overnight at 4\u00b0C or incubate at 37\u00b0C for 2 hours.<\/li>\n
  2. Aspirate and wash 1 time.<\/li>\n
  3. Block the plates with 200 \u03bcL per well of working solution of Blocking Buffer. Incubate at 37\u00b0C for 1.5 hours.<\/li>\n
  4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.<\/li>\n<\/ol>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Uncoated \u591a\u76e4\u5f0fELISA\u5546\u54c1\u5168\u65b0\u4e0a\u5e02!! (Uncoated) \u767d\u7d30\u80de\u4ecb\u7d204\uff08IL4\uff09ELISA\u8a66\u5291 […]<\/p>\n","protected":false},"author":3,"featured_media":16326,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[463],"tags":[2645],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17326"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17326"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17326\/revisions"}],"predecessor-version":[{"id":17329,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17326\/revisions\/17329"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16326"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17326"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17326"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17326"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}