{"id":17188,"date":"2018-06-27T11:11:34","date_gmt":"2018-06-27T03:11:34","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=17188"},"modified":"2025-03-21T14:46:54","modified_gmt":"2025-03-21T06:46:54","slug":"%e6%b0%b8%e7%94%9f%e5%8c%96%e5%b0%8f%e9%bc%a0%e8%82%ba%e4%b8%8a%e7%9a%ae%e7%b4%b0%e8%83%9e-immortalized-mouse-lung-epithelial-cells-mle-15-%e8%b2%a8%e8%99%9ft0495","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=17188","title":{"rendered":"\u6c38\u751f\u5316\u5c0f\u9f20\u80ba\u4e0a\u76ae\u7d30\u80de |Immortalized Mouse Lung Epithelial Cells (MLE-15) \u8ca8\u865fT0495"},"content":{"rendered":"

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong><\/p>\n

\u6c38\u751f\u5316\u5c0f\u9f20\u80ba\u4e0a\u76ae\u7d30\u80de\uff08MLE-15\uff09- SV40\u00a0|\u00a0<\/strong>(<\/strong>\u8ca8\u865f<\/strong>T0495)<\/strong><\/a><\/span><\/p>\n

Immortalized Mouse Lung Epithelial Cells (MLE-15)<\/p>\n

\u975e\u7e96\u6bdb\u6027\u7d30\u652f\u6c23\u7ba1\uff08Clara\uff09\u7d30\u80de\uff0c\u80ba\u6ce1I\u578b\u548c\u80ba\u6ce1II\u578b\u7d30\u80de\u662f\u547c\u5438\u9053\u4e0a\u76ae\u7d30\u80de\u4e2d\u7684\u4e3b\u8981\u7d30\u80de\u985e\u578b\u3002 \u514b\u62c9\u62c9\u7d30\u80de\u548c\u80ba\u6ce1\u2161\u578b\u7d30\u80de\u7522\u751f\u80ba\u8868\u9762\u6d3b\u6027\u86cb\u767d\uff0c\u80ba\u8868\u9762\u6d3b\u6027\u7269\u8cea\u86cb\u767d\u901a\u904e\u964d\u4f4e\u6c23\u6db2\u754c\u9762\u8655\u7684\u8868\u9762\u5f35\u529b\u800c\u5c0d\u80ba\u6ce1\u7d50\u69cb\u548c\u529f\u80fd\u7dad\u6301\u81f3\u95dc\u91cd\u8981\u3002 \u6c38\u751f\u5c0f\u9f20\u80ba\u4e0a\u76ae\u7d30\u80de\uff08MLE-15\uff09\u662f\u4f86\u81ea\u651c\u5e36\u5728\u4ebaSP-C\u555f\u52d5\u5b50\u63a7\u5236\u4e0b\u7684SV40\u6a19\u7c64\u7684\u8f49\u57fa\u56e0FVB \/ N\u5c0f\u9f20\u7684\u7d30\u80de\u7cfb\u3002 \u8207II\u578b\u80ba\u6ce1\u7d30\u80de\u985e\u4f3c\uff0cMLE-15\u7684\u7279\u5fb5\u5728\u65bc\u5b58\u5728\u9802\u7aef\u5fae\u7d68\u6bdb\u548c\u7d30\u80de\u5167\u591a\u5c64\u5305\u6db5\u9ad4\uff0c\u4ee5\u53ca\u7522\u751f\u8868\u9762\u6d3b\u6027\u86cb\u767dB\u548cC\u7684\u80fd\u529b\u3002\u8a72\u7d30\u80de\u7cfb\u7528\u65bc\u7814\u7a76\u80ba\u4e0a\u76ae\u57fa\u56e0\u7684\u8abf\u7bc0 \u4e26\u95e1\u660e\u8207\u6c23\u9053\/\u80ba\u6ce1\u5206\u5316\u76f8\u95dc\u7684\u8907\u96dc\u8f49\u9304\u7db2\u7d61\u3002<\/p>\n

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\u6c38\u751f\u5316\u5c0f\u9f20\u80ba\u4e0a\u76ae\u7d30\u80de\uff08MLE-15\uff09- SV40<\/strong>\u7d30\u80de\u7279\u6027: Adherent | \u4e0d\u6747\u65b9\u6cd5: The Immortalized Mouse Lung Epithelial Cells (MLE-15) is a cell line derived from transgenic FVB\/N mice harbouring SV40 Tag under the control of the human SP-C promoter. | \u4f86\u6e90SP-C\/TAg Transgenic Mice \u3002\u5668\u5b98\u4f86\u6e90Distal respiratory epithelium\u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrowIII (<\/u>\u8ca8\u865f<\/u>\u00a0<\/u>TM002<\/u>)<\/u>\u3002\u70ba\u4e86\u88fd\u5099\u5b8c\u6574\u7684\u751f\u9577\u57f9\u990a\u57fa\uff0c\u5c07\u4e0b\u5217\u7d44\u5206\u6dfb\u52a0\u5230\u57fa\u790e\u57f9\u990a\u57fa\u4e2d\uff1a\u80ce\u725b\u8840\u6e05\uff08TM999\uff09\u81f3\u6700\u7d42\u6fc3\u5ea62\uff05\uff0c5mg \/ L insulin\u80f0\u5cf6\u7d20\uff0c10\u03bcg\/ ml transferrin\u8f49\u9435\u86cb\u767d\uff0c3ng \/ ml sodium selenite\u4e9e\u7852\u9178\u9209\uff0c10 nM hydrocortisone\u6c2b\u5316\u53ef\u7684\u677e\uff0c10nM\u03b2-\u96cc\u4e8c\u9187\u548cPenicillin\/Streptomycin\u9752\u9ef4\u7d20\/\u93c8\u9ef4\u7d20\u6eb6\u6db2\uff08G255\uff09\u81f3\u6700\u7d42\u6fc3\u5ea6\u70ba1\uff05\u3002\u4e8c\u6c27\u5316\u78b3\uff08CO2\uff09\uff1a5\uff05\uff0c\u6eab\u5ea6\uff1a37.0\u2103\u3002<\/p>\n

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\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Organism<\/strong><\/td>\nSP-C\/TAg Transgenic Mice<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nDistal respiratory epithelium<\/td>\n<\/tr>\n
Donor Gender<\/strong><\/td>\nFemale<\/td>\n<\/tr>\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nPolygonal<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\nThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nNon-ciliated bronchiolar (Clara) cells, alveolar type I and alveolar type II cells are the predominant cell types in the respiratory epithelium. Clara cells and alveolar type II cells produce pulmonary surfactant proteins that are critical in the structure and functional maintenance of the pulmonary alveolus by reducing surface tension at the air-liquid interface. The Immortalized Mouse Lung Epithelial Cells (MLE-15) is a cell line derived from transgenic FVB\/N mice harbouring SV40 Tag under the control of the human SP-C promoter. Similar to alveolar type II cells, MLE-15 is characterized by the presence of apical microvilli and intracellular multilamellar inclusion bodies, as well as the ability to produce surfactant proteins B and C. This cell line is useful in studying the regulation of lung epithelial genes and elucidating the complex transcriptional networks related to airway\/alveolar differentiation.<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Markers<\/strong><\/td>\nSP-A (low level), SP-B, SP-C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7522\u54c1\u8cc7\u6599\u8acb\u6309\u9019PriCoat\u2122 T25 Flasks | type I collagen coating | <\/a>I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix | <\/a>Prigrow III Medium\u521d\u4ee3\u7d30\u80de\u57f9\u990a\u6db2 <\/a><\/p>\n

HOT!!<\/strong>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/p>\n

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Abmgood<\/strong>\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized reagenet)<\/strong>\u00a0Ready-to-use<\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1 (immortalized cell) \u6c38\u751f\u5316\u5c0f\u9f20\u80ba\u4e0a\u76ae\u7d30\u80de\uff08MLE-15\uff09- […]<\/p>\n","protected":false},"author":3,"featured_media":17189,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453],"tags":[2671],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17188"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17188"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17188\/revisions"}],"predecessor-version":[{"id":50828,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/17188\/revisions\/50828"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/17189"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17188"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17188"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17188"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}