{"id":16571,"date":"2018-05-15T13:25:06","date_gmt":"2018-05-15T05:25:06","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16571"},"modified":"2024-10-30T11:40:23","modified_gmt":"2024-10-30T03:40:23","slug":"%e5%8f%af%e6%ba%b6%e6%80%a7%e7%92%b0%e6%b0%a7%e5%8c%96%e7%89%a9%e6%b0%b4%e8%a7%a3%e9%85%b6%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-soluble-epoxide-hydrolase-activity-assay-kit","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=16571","title":{"rendered":"\u53ef\u6eb6\u6027\u74b0\u6c27\u5316\u7269\u6c34\u89e3\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | Soluble Epoxide Hydrolase Activity Assay Kit\u8ca8\u865fK477-100 (abcam ab240999)\u00a0"},"content":{"rendered":"

\u53ef\u6eb6\u6027\u74b0\u6c27\u5316\u7269\u6c34\u89e3\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08<\/strong>\u87a2<\/strong>\u5149\u6cd5\uff09 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K477-100 (abcam ab240999)\u00a0<\/a><\/span><\/p>\n

Soluble Epoxide Hydrolase Activity Assay Kit (Fluorometric)<\/em><\/strong><\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u9ad8\u9748\u654f\u5ea6\u6aa2\u6e2c\u53ef\u6eb6\u6027\u74b0\u6c27\u5316\u7269\u6c34\u89e3\u9176; \u5305\u62ecsEH\u967d\u6027\u5c0d\u7167\u3002\u53ef\u6eb6\u6027\u74b0\u6c27\u5316\u7269\u6c34\u89e3\u9176\u6216sEH\uff08EC 3.3.2.10\uff09\u662f\u4e00\u7a2e\u7d30\u80de\u6eb6\u8cea\u9176\uff0c\u5ee3\u6cdb\u5b58\u5728\u65bc\u5305\u62ec\u809d\u81df\u3001\u814e\u81df\u3001\u80f0\u5cf6\u3001\u8166\u5782\u9ad4\u3001\u6dcb\u5df4\u7d44\u7e54\u3001\u808c\u8089\u548c\u80c3\u8178\u9053\u7684\u5e7e\u7a2e\u5668\u5b98\u4e2d\u3002\u5b83\u50ac\u5316\u74b0\u6c27\u4e8c\u5341\u78b3\u4e09\u70ef\u9178\uff08EET\uff09\u7684\u6c34\u89e3\uff0c\u5373\u884d\u751f\u81ea\u7d30\u80de\u8272\u7d20P-450\u4ecb\u5c0e\u7684\u82b1\u751f\u56db\u70ef\u9178\u4ee3\u8b1d\u7684\u74b0\u6c27\u5316\u7269\uff0c\u4e26\u5f62\u6210\u9130\u4f4d\u4e8c\u9187\u3002 sEH\u5728\u6d89\u53ca\u75bc\u75db\u548c\u708e\u75c7\u7684\u5167\u6e90\u6027\u8102\u8cea\u4ee3\u8b1d\u4e2d\u8d77\u4e3b\u8981\u4f5c\u7528\u3002\u53ef\u6eb6\u6027\u74b0\u6c27\u5316\u7269\u6c34\u89e3\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2 (ab240999) \u662f\u4e00\u7a2e\u5fae\u5b54\u677f\u7528\u65bc\u6e2c\u91cf\u53ef\u6eb6\u6027\u74b0\u6c27\u5316\u7269\u6c34\u89e3\u9176 (sEH) \u7684\u87a2\u5149\u8a66\u5291\u76d2\u3002\u7528\u65bc\u6e2c\u91cf\u7d30\u80de\u548c\u7d44\u7e54\u4e2d\u7684sEH\u6d3b\u6027\u4ee5\u53ca\u7d14\u5316\u7684\u86cb\u767d\u8cea\u3002\u5b83\u57fa\u65bcsEH\u5c07\u975e\u87a2\u5149\u5e95\u7269\u6c34\u89e3\u6210\u87a2\u5149\u7522\u7269\u7684\u80fd\u529b\u3002\u8a72\u8a66\u5291\u76d2\u542b\u6709\u53ef\u6eb6\u6027\u74b0\u6c27\u5316\u7269\u6c34\u89e3\u9176\u7684\u7279\u7570\u6027\u6291\u5236\u5291\uff0c\u56e0\u70ba\u5e95\u7269\u53ef\u4ee5\u88ab\u5b58\u5728\u65bc\u7d30\u80de\u548c\u7d44\u7e54\u88c2\u89e3\u7269\u4e2d\u7684\u975e\u7279\u7570\u6027\u6c34\u89e3\u9176\u6c34\u89e3\u3002\u901a\u904e\u5f9e\u7e3d\u6d3b\u6027\u4e2d\u6e1b\u53bbsEH\u6291\u5236\u5291\u5b58\u5728\u4e0b\u7684\u6d3b\u6027\u53ef\u4ee5\u7372\u5f97\u7279\u5b9a\u7684sEH\u6d3b\u6027\u3002<\/p>\n

\"Functional<\/p>\n

\"Functional<\/p>\n

\"Functional<\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK477-100 (abcam ab240999)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method-Fluorescence (Ex\/Em: 362\/460 nm)
\n\u2022 Applications- Measurement of sEH activity in cell and tissue lysates using a 96-well plate format<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em: 362\/460 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nEukaryotes<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Rapid assessment of native\/recombinant sEH activity.
\n\u2022 \u00a0Soluble Epoxide Hydrolase activity in mammalian cell culture, and tissue lysates.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Rapid, simple & sensitive
\n\u2022 Convenient & HTS
\n\u2022 Includes specific sEH inhibitor<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 sEH Assay Buffer
\n\u2022 sEH Substrate
\n\u2022 sEH Inhibitor (5X)
\n\u2022 Fluorescence Standard
\n\u2022 sEH Positive Control<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

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