{"id":16165,"date":"2018-03-26T11:06:36","date_gmt":"2018-03-26T03:06:36","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=16165"},"modified":"2025-03-21T14:48:05","modified_gmt":"2025-03-21T06:48:05","slug":"%e6%b0%b8%e7%94%9f%e5%8c%96%e4%ba%ba%e7%89%99%e9%bd%a6%e8%a7%92%e8%b3%aa%e5%bd%a2%e6%88%90%e7%b4%b0%e8%83%9e-immortalized-human-gingival-keratinocytes-gie-no3b11-%e8%b2%a8%e8%99%9ft0049","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=16165","title":{"rendered":"\u6c38\u751f\u5316\u4eba\u7259\u9f66\u89d2\u8cea\u5f62\u6210\u7d30\u80de | Immortalized Human Gingival Keratinocytes (Gie-No3B11) (\u8ca8\u865fT0049)"},"content":{"rendered":"

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong><\/p>\n

\u6c38\u751f\u5316\u4eba\u7259\u9f66\u89d2\u8cea\u5f62\u6210\u7d30\u80de\uff08Gie-No3B11\uff09 <\/strong>(<\/strong>\u8ca8\u865f<\/strong>T0049)<\/strong><\/a><\/span><\/p>\n

Immortalized Human Gingival Keratinocytes (Gie-No3B11)\"\"<\/p>\n

\u7d30\u80de\u682a\u4ecb\u7d39<\/strong>:<\/strong> \u7259\u9f66\u4e0a\u76ae\u4fdd\u8b77\u7259\u5468\u7d44\u7e54\u4e0d\u53d7\u7d30\u83cc\u548c\u6709\u6bd2\u7269\u8cea\u7684\u5f71\u97ff\u3002 \u89d2\u8cea\u5f62\u6210\u7d30\u80de\u901a\u904e\u8207\u7d30\u80de\u9aa8\u67b6\u548c\u76f8\u9130\u7d30\u80de\u5f62\u6210\u7d30\u80de\u9593\u9023\u63a5\u800c\u5728\u4fc3\u9032\u5f37\u4e0a\u76ae\u9375\u65b9\u9762\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002 \u6c38\u751f\u5316\u4eba\u985e\u7259\u9f66\u89d2\u8cea\u5f62\u6210\u7d30\u80de\u662f\u7d93\u8f49\u67d3\u4ee5\u8868\u9054HPV-16E7\u7684\u5206\u96e2\u7684\u521d\u7d1a\u53e3\u8154\u89d2\u8cea\u5f62\u6210\u7d30\u80de\u3002 \u5b83\u8868\u9054\u7d30\u80de\u89d2\u86cb\u767d2\uff0c\u7d30\u80de\u89d2\u86cb\u767d17\uff0c\u7d30\u80de\u89d2\u86cb\u767d19\uff0c\u5305\u819c\u86cb\u767d\uff0c\u805a\u89d2\u86cb\u767d\u548cclaudin-1\u3002 \u9019\u4e9b\u7d30\u80de\u53ef\u4ee5\u901a\u904e\u6539\u8b8a\u57f9\u990a\u689d\u4ef6\u4f86\u5efa\u7acb\u8de8\u4e0a\u76ae\u96fb\u963b\uff0c\u9019\u662f\u7259\u9f66\u89d2\u8cea\u5f62\u6210\u7d30\u80de\u7684\u4e00\u500b\u7368\u7279\u7279\u5fb5\u3002\u9019\u4e9b\u7d30\u80de\u88ab\u63a8\u85a6\u7528\u65bc\u6709\u52a9\u65bc\u7259\u9f66\u7d44\u7e54\u7279\u5b9a\u6027\u8cea\u7684\u6a5f\u5236\u7684\u7814\u7a76\uff0c\u4f8b\u5982\u4e0a\u76ae\u5c4f\u969c\u529f\u80fd\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Organism<\/strong><\/td>\nHomo sapiens<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nBuccal Gingiva<\/td>\n<\/tr>\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nPolygonal<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\n5,000-7,000 cells\/ cm2<\/sup>; Recommended split ratio is 1:2 to 1:3<\/td>\n<\/tr>\n
Population Doubling<\/strong><\/td>\n32-42 hours<\/td>\n<\/tr>\n
Immortalization Method<\/strong><\/td>\nSerial passaging and chemical mediated transfection with plasmid carrying human papilloma virus (HPV-16) E6 and E7 genes<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nThe gingival epithelium protects periodontal tissue from bacteria and toxic substances. Keratinocytes play an important role in promoting strong epithelial bonds through the formation of intercellular connections with the cytoskeleton and neighboring cells. The Immortalized Human Gingival Keratinocytes are isolated primary oral keratinocytes transfected to express the HPV-16 E7. It expresses cytokeratin 2, cytokeratin 17, cytokeratin 19, involucrin, filaggrin, and claudin-1. These cells may be differentiated by altering culturing conditions to build transepithelial electrical resistance, a distinct characteristic of gingival keratinocytes. These cells are recommended for studies in mechanisms that contribute to the specific properties of the gingiva tissue, such as the epithelial barrier function.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6c38\u751f\u5316\u4eba\u7259\u9f66\u89d2\u8cea\u5f62\u6210\u7d30\u80de\uff08Gie-No3B11\uff09 <\/strong>\u7d30\u80de\u7279\u6027: Adherent\u578b\u614bPolygonal\u4f86\u6e90Buccal Gingiva\u3002Immortalization\u65b9\u5f0fSerial passaging and chemical mediated transfection with plasmid carrying human papilloma virus (HPV-16) E6 and E7 genes\u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002The base medium for this cell line is Keratinocyte Medium Kit available in abm, Cat. No TM040. Atmosphere: air: 95%, CO\u2082: 5%; Temperature: 37.0\u00b0C. To differentiate the cells: Add calcium to a final concentration of 1.8 mM to the complete growth medium.<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u8cc7\u6599\u8acb\u6309\u9019\u00a0PriCoat\u2122 T25 Flasks | type I collagen coating | <\/a>I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix | <\/a>Prigrow III Medium\u521d\u4ee3\u7d30\u80de\u57f9\u990a\u6db2 <\/a><\/span><\/p>\n

HOT!!<\/strong><\/span>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

Abmgood<\/strong>\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized reagenet)<\/strong>\u00a0Ready-to-use<\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1 (immortalized cell) \u6c38\u751f\u5316\u4eba\u7259\u9f66\u89d2\u8cea\u5f62\u6210\u7d30\u80de\uff08Gie-No3 […]<\/p>\n","protected":false},"author":3,"featured_media":16166,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[455],"tags":[2822],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16165"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=16165"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16165\/revisions"}],"predecessor-version":[{"id":50831,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/16165\/revisions\/50831"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16166"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=16165"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=16165"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=16165"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}