{"id":15911,"date":"2018-03-13T12:17:18","date_gmt":"2018-03-13T04:17:18","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15911"},"modified":"2025-08-20T15:46:39","modified_gmt":"2025-08-20T07:46:39","slug":"%e8%91%a1%e8%90%84%e7%b3%96%e6%af%94%e8%89%b2%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92ii-glucose-colorimetric-assay-kit-ii-%e8%b2%a8%e8%99%9fk686-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=15911","title":{"rendered":"\u8461\u8404\u7cd6\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2II | Glucose Colorimetric Assay Kit II \u8ca8\u865fK686-100(ABCAM ab102517)"},"content":{"rendered":"

\u8461\u8404\u7cd6\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2II <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K686-100(ABCAM ab102517)<\/a>\u00a0\u00a020\u03bcM – 10mM<\/span><\/span><\/p>\n

For samples having reducing substances<\/em><\/strong><\/p>\n

\u8461\u8404\u7cd6 (Glucose ) \u662f\u7522\u751f\u901a\u7528\u80fd\u91cf\u5206\u5b50ATP (\u00a0energy molecule ATP) \u7684\u91cd\u8981\u71c3\u6599\u4f86\u6e90\u3002 \u8840\u6e05\u8461\u8404\u7cd6 (Serum glucose) \u6c34\u5e73\u662f\u8a31\u591a\u4ee3\u8b1d\u7d0a\u4e82\u7684\u95dc\u9375\u8a3a\u65b7\u53c3\u6578\u3002 BioVision\u7684\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2II\u63d0\u4f9b\u5c0d\u5404\u7a2e\u751f\u7269\u6a23\u54c1\uff08\u4f8b\u5982serum\u8840\u6e05\uff0cplasma\u8840\u6f3f\uff0c\u5176\u4ed6\u9ad4\u6db2\uff0cfood\u98df\u7269\uff0cgrowth media\u751f\u9577\u57f9\u990a\u57fa\u7b49\uff09\u4e2d\u7684\u8461\u8404\u7cd6\u7684\u76f4\u63a5\u6e2c\u91cf\u3002 \u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u8461\u8404\u7cd6\u88ab\u7279\u7570\u6027\u6c27\u5316\u4ee5\u7522\u751f\u8207\u67d3\u6599\u53cd\u61c9\u7522\u751f\u5f37\u5ea6\u8207\u8461\u8404\u7cd6\u6fc3\u5ea6\u6210\u6bd4\u4f8b\u7684\u984f\u8272\uff08\u03bb= 450nm\uff09\u7684\u7522\u7269\u3002 \u8a72\u65b9\u6cd5\u5feb\u901f\uff0c\u7c21\u55ae\uff0c\u9748\u654f\uff0c\u9069\u7528\u65bc\u9ad8\u901a\u91cf\u3002 \u672c\u8a66\u5291\u76d2\u7279\u5225\u9069\u7528\u65bc\u00a0serum\u00a0\u8840\u6e05\u548curine\u00a0\u5c3f\u6db2\u6a23\u54c1\uff0c\u4e0d\u53d7\u9084\u539f\u6027\u7269\u8cea(\u00a0reducing substances )\u7684\u5f71\u97ff\u3002 \u8a72\u6e2c\u5b9a\u6cd5\u4e5f\u9069\u7528\u65bc\u76e3\u6e2c\u767c\u9175 (fermentation ) \u671f\u9593\u7684\u8461\u8404\u7cd6\u6c34\u5e73\u548c\u86cb\u767d\u8cea\u8868\u9054\u904e\u7a0b\u4e2d\u7684\u8461\u8404\u7cd6\u651d\u5165(glucose feeding ) \u3002 \u8a72\u8a66\u5291\u76d2\u53ef\u6aa2\u6e2c20\u03bcM – 10mM\u7bc4\u570d\u5167\u7684\u8461\u8404\u7cd6\u6fc3\u5ea6\u3002<\/p>\n

 <\/p>\n

\"ELISA<\/a><\/p>\n

ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a>\u00a0<\/a>\u00a0(\u6a19\u914d405\u3001450\u3001492\u3001630 nm\u6ffe\u7247\/\u6700\u9ad8\u53ef\u64f4\u5145\u81f37\u6ffe\u7247\uff09\uff08k686 \u03bb= 450 nm\uff09<\/span><\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK686-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe kit can detect glucose concentrations in the range of 20\u00b5M \u2013 10mM<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 30 minutes to complete the assay
\n\u2022 Fast and convenient<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Glucose Assay Buffer
\n\u2022 Glucose Substrate Mix
\n\u2022 Glucose Enzyme Mix
\n\u2022 Glucose Standard (100 mM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Biovision\u63d0\u4f9b3\u7a2e\u8461\u8404\u7cd6\u6fc3\u5ea6\u8a66\u5291\u76d2\u5546\u54c1\u3002K606-100\uff1a\u9069\u7528\u65bc\u6bd4\u8272\u548c\u87a2\u5149\u6aa2\u6e2c\u3002\u9700\u8981570 nm\u548cEx \/ Em\uff1a535\/587 nm\u7684\u6ffe\u5149\u7247. \u6e2c\u91cf\u4e4b\u9650\u5236\u70ba1-10000 uM\u3002 K686-100\u50c5\u9069\u7528\u65bc\u6bd4\u8272\u6aa2\u6e2c\uff0c\u6e2c\u91cf\u6ce2\u9577\u70ba450 nm\uff0c\u6e2c\u91cf\u7bc4\u570d\u70ba20uM\u81f310 mM\u3002\u8461\u8404\u7cd6\u7684\u6fc3\u5ea6\u9650\u5236\u4e0d\u4f7f\u7528\u6c27\u5316\u6216Picoprobe\uff08TM\uff09\u9069\u7528\u65bc\u5177\u6709\u9084\u539f\u7269\u8cea\u7684\u6a23\u54c1\u3002K688-100\u50c5\u9069\u7528\u65bc\u87a2\u5149\u6aa2\u6e2c\uff0c\u6fc0\u767c\u5149\u53ca\u6563\u5c04\u5149\u8a2d\u5b9a\u70baEx \/ Em\uff1a535\/587nm\u3002\u8461\u8404\u7cd6\u7684\u6fc3\u5ea6\u9650\u5236<0.5uM\u3002<\/p>\n

 <\/p>\n

\n
Ling, Z. et al., (2017) miR-361-5p modulates metabolism and autophagy via the Sp1-mediated regulation of PKM2 in prostate cancer,\u00a0Oncology Reports, Jul.2017, 38(3), 1621-1628<\/a><\/div>\n<\/div>\n
\n
Li et al., Crucial role of TRPC6 in maintaining the stability of HIF-1\u03b1 in glioma cells under hypoxia.\u00a0J. Cell Sci.,\u00a0Sep 2015; 128: 3317 – 3329.<\/a><\/div>\n<\/div>\n
\n
Shi et al., A Novel KLF4\/LDHA Signaling Pathway Regulates Aerobic Glycolysis in and Progression of Pancreatic Cancer.\u00a0Clin. Cancer Res.,\u00a0Aug 2014; 20: 4370 – 4380.<\/a><\/div>\n<\/div>\n
\n
Makinoshima et al., Epidermal Growth Factor Receptor (EGFR) Signaling Regulates Global Metabolic Pathways in EGFR-mutated Lung Adenocarcinoma.\u00a0J. Biol. Chem.,\u00a0Jul 2014; 289: 20813 – 20823.<\/a><\/div>\n<\/div>\n
\n
Cui et al., FOXM1 Promotes the Warburg Effect and Pancreatic Cancer Progression via Transactivation of LDHA Expression.\u00a0Clin. Cancer Res.,\u00a0May 2014; 20: 2595 – 2606.<\/a><\/div>\n<\/div>\n

 <\/p>\n

\u00a0\"smart\"<\/a>\"Spectrumlab<\/a>\"102-T\"<\/a>\"0100-01\"<\/a><\/strong><\/p>\n

\u00a0\"\u540c\u8cea\u5546\u54c17\"<\/a><\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u8461\u8404\u7cd6\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2II | \u8ca8\u865fK686-100(ABCAM ab102517)\u00a0\u00a020\u03bcM – […]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[467,124,180],"tags":[2858],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15911"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15911"}],"version-history":[{"count":5,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15911\/revisions"}],"predecessor-version":[{"id":52394,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15911\/revisions\/52394"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15911"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15911"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15911"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}