{"id":15899,"date":"2018-03-13T10:45:19","date_gmt":"2018-03-13T02:45:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15899"},"modified":"2025-03-21T14:49:39","modified_gmt":"2025-03-21T06:49:39","slug":"%e6%b0%b8%e7%94%9f%e5%b0%8f%e9%bc%a0%e8%a6%96%e7%b6%b2%e8%86%9c%e8%89%b2%e7%b4%a0%e4%b8%8a%e7%9a%ae%e7%b4%b0%e8%83%9e-hpv16-e6-e7-human-olfactory-ensheathing-glia-bmi1htert-%e8%b2%a8%e8%99%9ft0","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=15899","title":{"rendered":"\u6c38\u751f\u5c0f\u9f20\u8996\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de – HPV16 E6 \/ E7 | Human Olfactory Ensheathing Glia Bmi1\/hTERT \u8ca8\u865fT0574"},"content":{"rendered":"

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong><\/p>\n

\u6c38\u751f\u5c0f\u9f20\u8996\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de – HPV16 E6 \/ E7 <\/strong>(<\/strong>\u8ca8\u865fT0574)<\/strong><\/a><\/span><\/p>\n

\"\"<\/p>\n

Immortalized Human Olfactory Ensheathing Glia Bmi1\/hTERT<\/p>\n

\u6c38\u751f\u5c0f\u9f20\u8996\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de-HPV E6 \/ E7\uff0c\u6e90\u81ea\u5065\u5eb7\u7684C57B1 \/ 6\u5c0f\u9f20RPE\u7d30\u80de\uff0c\u662f\u4e00\u7a2e\u4fdd\u7559\u5176\u8a31\u591a\u9ad4\u5167\u8868\u578b\u7279\u5fb5\u7684\u7d30\u80de\u7cfb\u3002 RPE\u7279\u7570\u6027\u6a19\u8a18\u5982RPE65\u548c\u7d30\u80de\u8996\u9ec3\u919b\u7d50\u5408\u86cb\u767d\uff08CRALBP\uff09\u4ee5\u53ca\u4e0a\u76ae\u6a19\u8a18ZO1\uff0c\u7d30\u80de\u89d2\u86cb\u767d8\u548c18\u5728\u6c38\u751f\u5316\u5c0f\u9f20\u8996\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de-HPV E6 \/ E7\u4e2d\u8868\u9054\u3002 \u9019\u4e9b\u7d30\u80de\u53ef\u7528\u65bc\u7814\u7a76RPE\u7d30\u80de\u751f\u7269\u5b78\u548c\u5305\u62ec\u5e74\u9f61\u76f8\u95dc\u6027\u9ec3\u6591\u8b8a\u6027\uff08AMD\uff09\u5728\u5167\u7684\u773c\u90e8\u75be\u75c5\u7684\u7814\u7a76\u3002<\/p>\n

\"\"<\/p>\n

\u6c38\u751f\u5c0f\u9f20\u8996\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de – HPV16 E6 \/ E7<\/strong> \u7d30\u80de\u7279\u6027: Adherent | \u4e0d\u6747\u65b9\u6cd5: retrovirus carrying human papiloma virus 16 E6\/E7 | \u4f86\u6e90Retina \u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrowIII (<\/u>\u8ca8\u865f<\/u>\u00a0<\/u>TM003<\/u>)<\/u>\uff0c\u80ce\u725b\u8840\u6e05(Cat. No. TM999<\/a>)\u6700\u7d42\u6fc3\u5ea6\u70ba10%\u4e26\u586b\u52a01% Penicillin\/Streptomycin \u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Organism<\/strong><\/td>\nMus musculus C57B1\/6<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nRetina<\/td>\n<\/tr>\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nCobblestone-like<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\nThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.<\/td>\n<\/tr>\n
Immortalization Method<\/strong><\/td>\nSerial passaging and transduction with retrovirus carrying human papiloma virus 16 E6\/E7<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Markers<\/strong><\/td>\n1. RPE65 and CRALBP (Differentiated RPE)
\n2. ZO1, Cytokeratin -8 and -18 (Epithelial cell marker)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6c38\u751f\u5c0f\u9f20\u8996\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de – HPV16 E6 \/ E7<\/strong> \u578b\u614b\u70baAdherent\u3002\u7d30\u80de\u57f9\u990a\u6db2\u66f4\u63db\u5927\u7d041\u90312-3\u6b21\uff1b\u7d30\u80de\u7e7c\u4ee3\u70ba\u80f0\u86cb\u767d\u9176\u6eb6\u6db20.25% trypsin(TM050) \u7684\u4e0b37\u2103\u4e0bincubator 3-5\u5206\u9418\uff0c\u76f4\u5230\u7d30\u80de\u74b0\u7e5e\uff0c\u5728\u986f\u5fae\u93e1\u4e0b\u4e0d\u6642\u76e3\u6e2c\u3002\u7d30\u80de\u5916\u57fa\u8ceaCoating dish\uff0c\u7528PBS\u7a00\u91cbG422\uff081\uff1a1\uff09\u4e26\u5857\u8986\u66f4\u5c11\u7684\u6642\u9593\u3002\u81a0\u539f\u86cb\u767d\u542b\u91cf\u8f03\u9ad8\uff0c\u5f9e\u800c\u4f7f\u8207\u7d30\u80de\u7684\u7d50\u5408\u66f4\u5f37\u3002\u53ef\u4ee5\u5617\u8a66\u6e1b\u5c11incubator\u6642\u9593\u4ee5\u53ca\u5857\u8986\u66f4\u8584\u7684\u5c64\u3002\u6c38\u751f\u5c0f\u9f20\u8996\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de – HPV16 E6 \/ E7<\/strong>\u54c1\u8cea\u9451\u5b9a: \u00a0Western Blot\u7528\u65bc\u9a57\u8b49\u6c38\u751f\u5316\u7d30\u80de\u7cfb\u4e2dHPV\u86cb\u767d\u7684\u8868\u9054\u3002 2.\u4f7f\u7528Western\u5370\u8de1\u548c\u5be6\u6642RT-PCR\u4f86\u78ba\u8a8d\u9ad4\u5167RPE\u6a19\u8a8c\u7269\u5982RPE65\u548cCRALBP\u7684\u5b58\u5728\u3002 3.\u514d\u75ab\u7192\u5149\u67d3\u8272\u78ba\u8a8d\u4e0a\u76ae\u7d30\u80de\u6a19\u8a8c\u7269ZO1\uff0c\u7d30\u80de\u89d2\u86cb\u767d8\u548c\u7d30\u80de\u89d2\u86cb\u767d18\u7684\u8868\u9054\u30024.\u4e0d\u5b58\u5728\u795e\u7d93\u7d30\u80de\u6a19\u8a8c\u7269\uff0cSV2\uff0cPKC\u03b1\uff0c\u8996\u7d2b\u7d05\u8cea\u3002<\/p>\n

\u7522\u54c1\u8cc7\u6599\u8acb\u6309\u9019PriCoat\u2122 T25 Flasks | type I collagen coating | <\/a>I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix | <\/a>Prigrow III Medium\u521d\u4ee3\u7d30\u80de\u57f9\u990a\u6db2 <\/a><\/p>\n

\"\"<\/p>\n

HOT!!<\/strong>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

Abmgood<\/strong>\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized reagenet)<\/strong>\u00a0Ready-to-use<\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1 (immortalized cell) \u6c38\u751f\u5c0f\u9f20\u8996\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de &#8211 […]<\/p>\n","protected":false},"author":3,"featured_media":15901,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[455],"tags":[2860],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15899"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15899"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15899\/revisions"}],"predecessor-version":[{"id":50835,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15899\/revisions\/50835"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/15901"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15899"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15899"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15899"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}