{"id":15363,"date":"2018-01-18T12:00:56","date_gmt":"2018-01-18T04:00:56","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15363"},"modified":"2022-07-20T17:51:10","modified_gmt":"2022-07-20T09:51:10","slug":"scrambled-sgrna-crispr-lentivirus-target-sequence-gcactcacatcgctacatca-%e8%b2%a8%e8%99%9fk019","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=15363","title":{"rendered":"Scrambled sgRNA CRISPR Lentivirus | Target Sequence GCACTCACATCGCTACATCA \u8ca8\u865fK019"},"content":{"rendered":"

\"CRISPR<\/p>\n

Scrambled sgRNA CRISPR Lentivirus<\/strong> | <\/strong>\u8ca8\u865f<\/strong>K019<\/a><\/span><\/p>\n

\u5373\u7528\u578bLentivirus\u63d0\u4f9b\u529b\u50f9107<\/sup>\u00a0IU\/ml, 300ul<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>: \u00a0<\/strong>Abmgood\u63d0\u4f9b\u4e00\u7cfb\u5217\u91dd\u5c0d\u4e0d\u540cCRISPR\u76ee\u6a19\u5e8f\u5217, \u8a2d\u8a08sgRNA-CRISPR Lentivirus\u8868\u73fe\u8f09\u9ad4\u3002\u8d85\u904e90\uff05\u7684\u69cb\u5efa\u9ad4\u5c07\u572820-90\uff05\u7684\u7d30\u80de\u4e2d\u5b8c\u5168\u6572\u9664\u3002 \u5927\u591a\u6578\u69cb\u5efa\u9ad4> 50\uff05 \u00a0transduced\u8f49\u5c0e\u548cselected cells\u3002 \u8d8a\u4f86\u8d8a\u591a\u8b49\u64da\u8868\u660e\uff0csgRNA\u6bd4shRNA\u548csiRNA\u6572\u9664\u6a5f\u7387\u9ad8\u3002\u8cfc\u8cb7sgRNA Vector, \u5982\u679c\u8a2d\u8a08\u7528\u65bcCas9\u6838\u9178\u9176\u7684sgRNA\u69cb\u5efa\u9ad4\u6c92\u6709\u5408\u7406\u7684\u6572\u9664\uff0c\u5c07\u514d\u8cbb\u8a2d\u8a08\u53e6\u4e00\u7d443\u500b\u69cb\u5efa\u9ad4\u3002\u9700\u78ba\u4fdd\u60a8\u76e1\u53ef\u80fd\u5730\u512a\u5316\u4e86\u60a8\u7684\u5be6\u9a57\uff0c\u5305\u62ec\uff08\u5982\u9069\u7528\uff09\u589e\u52a0\u60a8\u7684MOI\uff08\u6700\u591a10\u6b21\uff09\u548c\u611f\u67d3\u6301\u7e8c\u6642\u9593\uff08\u6700\u591a72\u5c0f\u6642\uff09\uff0c\u4e26\u5728\u9032\u884cclone screening\u514b\u9686\u7be9\u9078\u3002<\/p>\n

\u65bc\u7cfb\u7d71\u9577\u975e\u7de8\u78bcRNAs (long non-coding RNAs): \u8a72\u7cfb\u7d71\u53ef\u4ee5\u7528\u65bc\u901a\u904e\u4f7f\u7528\u4e00\u5c0dsgRNA\u6572\u9664\u9577\u7684\u975e\u7de8\u78bcRNA\u6216miRNA\u6216\u4efb\u4f55\u77ed\u7247\u6bb5\u7684DNA\u3002 \u4f46\u662f\uff0c\u6548\u7387\u5c07\u6bd4\u6572\u9664\u7de8\u78bc\u86cb\u767d\u8cea\u7684\u57fa\u56e0\u4f4e\u5f97\u591a\uff0c\u7d04\u70ba1\uff05\u3002 \u56e0\u6b64\uff0c\u9700\u8981\u55ae\u7d30\u80de\u514b\u9686\u3002 \u53ef\u80fd\u8a2d\u8a083\u500b\u9776\u5411lncRNA\u958b\u59cb\u548c\u7d50\u675f\u7684sgRNA\uff083×2\u7d50\u69cb\uff09\u30023×3\u7684\u7d44\u5408\uff0c\u7528\u65bc\u6572\u9664\u9776\u5411\u7684lncRNA\uff0cPCR\u7be9\u9078\u9451\u5b9a\u70ba\u6700\u4f73\u7d44\u5408\u3002\u53ef\u4ee5\u5728\u8f49\u5c0e2\u500b\u6700\u4f73sgRNA\u5f8c\u9032\u884c\u55ae\u7d30\u80de\u514b\u9686\u3002\u9810\u8a081-5\uff05\u7684\u514b\u9686\u4e2d\uff0clncRNA\u5c07\u88ab\u5b8c\u5168\u6572\u9664\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Species<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npLenti-U6-sgRNA-PGK-Neo<\/a><\/td>\n<\/tr>\n
Target Sequence<\/strong><\/td>\nGCACTCACATCGCTACATCA<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nVirus<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nLentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80\u00b0C in small aliquots to avoid repeated freeze-thaw cycles.<\/td>\n<\/tr>\n
Shelf Life<\/strong><\/td>\n1 year (when at -80\u00b0C or below in a non-frost free freezer)<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nShipped with dry ice<\/td>\n<\/tr>\n
Additional Materials<\/strong><\/td>\n–\u00a0CRISPR Genomic Cleavage Detection Kit (G932)<\/a><\/td>\n<\/tr>\n
Quality Control<\/strong><\/td>\nRestriction Enzyme Digest and Sequencing<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Jiang, G\u00a0et al<\/i>. “Isorhapontigenin (ISO) inhibits invasive bladder cancer (BC) formation in vivo and human BC invasion in vitro by targeting STAT1\/FOXO1 Axis.” Cancer Prev Res. 9(7):567-580 (2016).\u00a0DOI: 10.1158\/1940-6207.CAPR-15-0338<\/a>.<\/p>\n

Sun, J\u00a0et al<\/i>. “Protein Phosphatase, Mg2+\/Mn2+-dependent 1A controls the innate antiviral and antibacterial response of macrophages during HIV-1 and Mycobacterium tuberculosis infection.” Oncotarget 7 (13):15394-409 (2016).\u00a0DOI: 10.18632\/oncotarget.8190<\/a>.\u00a0PubMed: 27004401<\/a>. Application: CRISPR KO.<\/p>\n

Kang, YJ\u00a0et al<\/i>. “Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling.” Nat. Commun. 6:8371 (2015).\u00a0DOI: 10.1038\/ncomms9371<\/a>.<\/p>\n

Okugawa, Y\u00a0et al<\/i>. “Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer.” Gut : (2015).\u00a0DOI: 10.1136\/gutjnl-2015-309359<\/a>.\u00a0PubMed: 26475630<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"

Scrambled sgRNA CRISPR Lentivirus | \u8ca8\u865fK019 \u5373\u7528\u578bLentiviru […]<\/p>\n","protected":false},"author":3,"featured_media":15364,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[522],"tags":[2961],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15363"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15363"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15363\/revisions"}],"predecessor-version":[{"id":15366,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15363\/revisions\/15366"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/15364"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15363"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15363"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15363"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}