{"id":153,"date":"2013-12-16T00:53:33","date_gmt":"2013-12-15T16:53:33","guid":{"rendered":"http:\/\/biopion.kc1982.org\/?p=153"},"modified":"2022-09-27T09:58:29","modified_gmt":"2022-09-27T01:58:29","slug":"rapid_microbe","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=153","title":{"rendered":"\u3010\u5fae\u751f\u7269\u51b7\u5149\u5b9a\u91cf\u3011\u5fae\u751f\u7269\u5feb\u901f\u5b9a\u91cf\u5957\u7d44\uff0dBiothema"},"content":{"rendered":"

\"biothema_header\"\u3000\u3000BioThema\u65bc2001\u5e74\u5efa\u7acb\u65bc\u745e\u5178\uff0c\u5c08\u9580\u7814\u767c\u751f\u7522\u51b7\u5149\uff08Bioluminescence\uff09\u4e4b\u76f8\u95dc\u7522\u54c1\uff0c\u63d0\u4f9b\u9ad8\u54c1\u8cea\u3001\u9ad8\u7a69\u5b9a\u6027\u4e4bATP\u8207\u51b7\u5149\u5206\u6790\u8a66\u5291\u5957\u7d44\u3002\u5176\u61c9\u7528\u5305\u62ec\u5feb\u901f\u7d30\u80de\u8a08\u6578\u3001\u7d30\u80de\u589e\u751f\u8207\u6bd2\u6027\u3001\u514d\u75ab\u8a66\u9a57\u3001\u9175\u7d20\u8207\u4ee3\u8b1d\u7269\u76e3\u63a7\u3001Luciferase reporter geneassay\u3001\u74b0\u5883\u885b\u751f\u6aa2\u6e2c\u7b49\u5206\u6790\u3002<\/span><\/p>\n

\u3000\u3000ATP\u751f\u7269\u51b7\u5149\u53cd\u61c9\u6280\u8853\u662f\u4e00\u7a2e\u5feb\u901f\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u7528\u4ee5\u6aa2\u6e2c\u751f\u7269ATP\u7684\u542b\u91cf\u3002\u6b64\u65b9\u6cd5\u70ba\u7f8e\u570b\u592a\u7a7a\u7e3d\u7f72\u65bc1960\u5e74\u4ee3\u767c\u5c55\u51fa\u4f86\u7684\u6280\u8853\uff0c\u7576\u521d\u767c\u5c55\u6b64\u9805\u6280\u8853\u7684\u76ee\u7684\u662f\u5e0c\u671b\u80fd\u7528\u4ee5\u6aa2\u6e2c\u5916\u592a\u7a7a\u662f\u5426\u6709\u751f\u547d\u7269\u8cea\u5b58\u5728\uff0c\u5176\u53cd\u61c9\u539f\u7406\u8207\u87a2\u706b\u87f2\u9ad4\u5167\u767c\u5149\u53cd\u61c9\u76f8\u540c\uff0c\u5728\u87a2\u5149\u7d20\uff08luciferin\uff09\u8207\u87a2\u5149\u9175\u7d20\uff08luciferase\uff09\u5b58\u5728\u4e0b\uff0cATP\u8207luciferin\u53caluciferase\u50ac\u5316\u4e0b\u7522\u751f\u6de1\u85cd\u8272\u7684\u5149\u3002\u53cd\u61c9\u65b9\u7a0b\u5f0f\u5982\u4e0b\uff1a<\/p>\n

\"rapid_ATP_formula\"<\/p>\n

\u3000\u3000\u8fd1\u4f86\u751f\u7269ATP\u51b7\u5149\u53cd\u61c9\u5728\u98df\u54c1\u8207\u91ab\u85e5\u9818\u57df\u4e2d\u88ab\u5ee3\u6cdb\u4f7f\u7528\u3002\u4ee5\u539f\u6838\u751f\u7269\u800c\u8a00\uff0c\u4e00\u96bb\u7d30\u83cc\u9ad4\u5167\u7d04\u542b2 x 10-18<\/sup> mol ATP\uff1b\u771f\u83cc\u7d30\u80de\u5247\u542b10-15<\/sup>\uff5e10-16<\/sup> mol ATP\u3002\u61c9\u7528ATP\u51b7\u5149\u53cd\u61c9\u6280\u8853\u6aa2\u6e2c\u751f\u83cc\u6578\u6642\uff0c\u9700\u5148\u5c07\u98df\u54c1\u4e2d\u975e\u5fae\u751f\u7269\u7684ATP\u6c34\u89e3\uff0c\u518d\u5c07\u5fae\u751f\u7269\u9ad4\u5167\u7684ATP\u91cb\u653e\u51fa\u4f86\uff0c\u53cd\u61c9\u5f8c\u4ee5ATP\u751f\u7269\u51b7\u5149\u5100\u5075\u6e2c\u3002\u82e5\u6aa2\u6e2c\u98df\u54c1\u63a5\u89f8\u8868\u9762\u7684\u6e05\u6f54\u5ea6\uff0c\u5247\u7121\u9700\u53bb\u9664\u98df\u54c1\u4e2d\u7684ATP\uff0c\u76f4\u63a5\u6e2c\u91cf\u5373\u53ef\u3002\u6aa2\u6e2c\u7d50\u679c\u5247\u4ee5\u76f8\u5c0d\u5438\u5149\u503c\uff08relative light unit, RLU\uff09\u7684\u591a\u5be1\u4f86\u8868\u793aATP\u6fc3\u5ea6\u7684\u9ad8\u4f4e\uff0c\u85c9\u6b64\u53cd\u63a8\u5fae\u751f\u7269\u542b\u91cf\u548c\u53ef\u80fd\u7684\u98df\u7269\u6b98\u7559\uff0c\u4ee5\u8868\u793a\u98df\u7269\u63a5\u89f8\u9762\u6e05\u6f54\u5ea6\u7684\u60c5\u6cc1\u3002<\/p>\n

 <\/p>\n

\u3000\u3000\u76ee\u524dATP\u751f\u7269\u51b7\u5149\u53cd\u61c9\u6280\u8853\u61c9\u7528\u5728\u5404\u9805\u98df\u54c1\u7522\u696d\uff0c\u5305\u62ec\u751f\u4e73\u751f\u83cc\u6578\u4f30\u7b97\u3001\u74b0\u5883\u8a2d\u5099\u6e05\u6f54\u8a55\u4f30\u3001\u755c\u7522\u696d\u4e2d\u5c60\u9ad4\u7684\u6c61\u67d3\u8a55\u4f30\u3001\u679c\u6c41\u7522\u696d\u7684\u751f\u83cc\u6578\u4f30\u7b97\u3001\u88fd\u9152\u5de5\u696d\u53ca\u98f2\u6599\u696d\u7b49\u3002<\/p>\n

 <\/p>\n

\u5fae\u751f\u7269\u5feb\u901f\u5b9a\u91cf\u5957\u7d44\u7522\u54c1\u5206\u985e\uff1a<\/span><\/p>\n

\"rapid_atp_chart\"<\/a><\/p>\n\n\n\n\n\n\n
\n

\u7522\u54c1\u7de8\u865f<\/strong><\/p>\n<\/td>\n

\n

\u54c1\u540d<\/strong><\/p>\n<\/td>\n

\n

\u898f\u683c<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

266-112<\/p>\n<\/td>\n

Microbial ATP Kit HS<\/td>\n\n

375 test<\/p>\n<\/td>\n<\/tr>\n

\n

266-111<\/p>\n<\/td>\n

Intracellular ATP Kit HS<\/td>\n\n

375 test<\/p>\n<\/td>\n<\/tr>\n

\n

266-311<\/p>\n<\/td>\n

ATP Biomass Kit HS<\/td>\n\n

375 test<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u3000\u3000BioThema\u65bc2001\u5e74\u5efa\u7acb\u65bc\u745e\u5178\uff0c\u5c08\u9580\u7814\u767c\u751f\u7522\u51b7\u5149\uff08Bioluminescence\uff09\u4e4b\u76f8\u95dc\u7522\u54c1\uff0c\u63d0\u4f9b […]<\/p>\n","protected":false},"author":6,"featured_media":605,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[19,123,550],"tags":[4847],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/153"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=153"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/153\/revisions"}],"predecessor-version":[{"id":12343,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/153\/revisions\/12343"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/605"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=153"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=153"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=153"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}