{"id":15254,"date":"2017-12-05T16:24:47","date_gmt":"2017-12-05T08:24:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15254"},"modified":"2023-03-13T18:11:13","modified_gmt":"2023-03-13T10:11:13","slug":"%e5%85%8d%e7%96%ab%e6%b2%89%e6%be%b1%ef%bc%88ip%ef%bc%89%e8%a9%a6%e5%8a%91%e7%9b%92-immunoprecipitation-ip-kit%e8%b2%a8%e8%99%9fk286-25","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=15254","title":{"rendered":"\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u8a66\u5291\u76d2 | Immunoprecipitation (IP) Kit\u8ca8\u865fK286-25(abcam ab206996)"},"content":{"rendered":"

\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u8a66\u5291\u76d2Immunoprecipitation (IP) Kit <\/strong>| <\/strong>\u8ca8\u865f<\/strong>K286-25<\/a>\u00a0(abcam ab206996)<\/span><\/p>\n

\u00a0\"\"<\/em><\/strong><\/p>\n

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\u514d\u75ab\u6c89\u6fb1 – \u514d\u75ab\u6c89\u6fb1\u8a66\u5291\u76d2 (ab206996)<\/strong><\/span><\/div>\n
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\u5171\u514d\u75ab\u6c89\u6fb1\u5be6\u9a57\u8868\u660e IKK\u03b5 \u4e0d\u8207 NIK \u6216 IKK\u03b5 \u76f8\u4e92\u4f5c\u7528\u3002\u6839\u64da\u88fd\u9020\u5546\u7684\u8aaa\u660e\uff0c\u4f7f\u7528 Abcam \u514d\u75ab\u6c89\u6fb1\u8a66\u5291\u76d2 (ab206996) \u5c0d MDA MB 468 \u7d30\u80de\u9032\u884c\u4e86\u514d\u75ab\u6c89\u6fb1\u3002\u7c21\u800c\u8a00\u4e4b\uff0c\u4f7f\u7528\u975e\u8b8a\u6027\u88c2\u89e3\u7de9\u885d\u6db2\u6536\u96c6 300 \u00b5g \u7d30\u80de\u88c2\u89e3\u7269\uff0c\u5c07\u5176\u8207 3 \u00b5g\/ml \u5c0d\u7167\u5154 IgG \u6216 IKK\u03b5 \u5154\u591a\u514b\u9686\u6297\u9ad4 (\u00a0ab7891<\/a>\u00a0) O\/N\u3002\u4f7f\u7528\u86cb\u767d A\/G \u74ca\u8102\u7cd6\u73e0\u6355\u7372\u6297\u9ad4\u7d50\u5408\u86cb\u767d\uff0c\u6d17\u812b\uff0c\u4e26\u901a\u904e\u86cb\u767d\u8cea\u5370\u8de1\u5206\u6790\u3002<\/p>\n<\/div>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u5ee3\u6cdb\u7528\u65bc\u7814\u7a76\u548c\u958b\u767c\uff0c\u901a\u904e\u9019\u7a2e\u65b9\u6cd5\u53ef\u4ee5\u5f9e\u6a23\u54c1\u4e2d\u9078\u64c7\u6027\u7d14\u5316\u86cb\u767d\u8cea\u3002 BioVision\u7684\u514d\u75ab\u6c89\u6fb1\u8a66\u5291\u76d2\u70ba\u88fd\u5099\u7d30\u80de\/\u7d44\u7e54\u63d0\u53d6\u7269\uff0c\u6297\u539f\u7d50\u5408\u548c\u6d17\u6ecc\u6b65\u9a5f\u63d0\u4f9b\u4e86\u512a\u5316\u7684\u7de9\u885d\u6db2\u3002 \u8a66\u5291\u76d2\u4e2d\u63d0\u4f9b\u7684\u86cb\u767d\u8ceaA \/ G\u74ca\u8102\u7cd6\u73e0\u8207\u50b3\u7d71\u86cb\u767dA\u6216\u86cb\u767dG\u6a39\u8102\u76f8\u6bd4\u5177\u6709\u66f4\u9ad8\u7684\u7d50\u5408\u80fd\u529b\uff0c\u5177\u6709\u66f4\u5ee3\u6cdb\u7684\u6297\u9ad4\u540c\u7a2e\u578b\u7d50\u5408\u3002 \u8a72\u8a66\u5291\u76d2\u53ef\u7528\u65bc\u591a\u7a2e\u514d\u75ab\u6c89\u6fb1\u6216Co-IP\u7814\u7a76\u3002<\/p>\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK286-25 (abcam ab206996)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n25 assays<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nImmunoprecipitation (IP) and Co-IP
\nFunctional study of Immunoprecipitated proteins\/complexes
\nSDS-PAGE or western blot analysis of Immunoprecipitated proteins\/complexes<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Provides optimized buffers for preparing cell\/tissue extracts, antigen binding and washing steps
\n\u2022 The protein A\/G Sepharose beads provided in the kit has higher binding capacity with broader antibody isotype binding than traditional protein A or protein G resins
\n\u2022 Validated for functional assays as well as analysis of IP\/Co-IP proteins\/complexes<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Non-Denaturing Lysis Buffer
\n\u2022 RIPA Lysis Buffer
\n\u2022 Protease Inhibitor Cocktail (lyophilized)
\n\u2022 10X Wash Buffer
\n\u2022 Protein A\/G Sepharose<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u514d\u75ab\u6c89\u6fb1\uff08IP\uff09\u8a66\u5291\u76d2Immunoprecipitation (IP) Kit | \u8ca8\u865fK286-25\u00a0(ab […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[3005],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15254"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15254"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15254\/revisions"}],"predecessor-version":[{"id":40037,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15254\/revisions\/40037"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15254"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15254"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15254"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}