{"id":15123,"date":"2017-11-13T15:20:56","date_gmt":"2017-11-13T07:20:56","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15123"},"modified":"2022-07-21T09:41:12","modified_gmt":"2022-07-21T01:41:12","slug":"nlrp1-lentiviral-vector-human-cumate-%e8%b2%a8%e8%99%9ficu2055228","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=15123","title":{"rendered":"NLRP1 Lentiviral Vector (Human) (Cumate) | \u8ca8\u865fiCu2055228"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=15123\">NLRP1 Lentiviral Vector (Human) (Cumate) | \u8ca8\u865fiCu2055228<\/a><\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/premadeLenti\/images\/Lentivector-workflow-cumate.png\" alt=\"\" width=\"690\" height=\"186\" \/><\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td><strong>Product Application<\/strong><\/td>\n<td>\n<p>\u00b7\u00a0 Direct non-viral plasmid transfection for immediate expression<\/p>\n<p>\u00b7\u00a0 Package into Lentiviral particles for high efficiency transduction and stably integrated expression<\/td>\n<\/tr>\n<tr>\n<td><strong>Gene Insert<\/strong><\/td>\n<td>NLRP1<\/td>\n<\/tr>\n<tr>\n<td><strong>Accession Number<\/strong><\/td>\n<td><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/nuccore\/BC016965\">BC016965<\/a><sup>1,2,3,4,5<\/sup><\/td>\n<\/tr>\n<tr>\n<td><strong>Vector<\/strong><\/td>\n<td><a href=\"https:\/\/www.abmgood.com\/NLRP1-Lentivirus-System-iCu2055228.html\">Cumate-pLenti-Cloning-SV40-GFP<\/a><\/td>\n<\/tr>\n<tr>\n<td><strong>Vector Size<\/strong><\/td>\n<td>8830 bp<\/td>\n<\/tr>\n<tr>\n<td><strong>Insert Size<\/strong><\/td>\n<td>441 bp<\/td>\n<\/tr>\n<tr>\n<td><strong>Species<\/strong><\/td>\n<td>Human<\/td>\n<\/tr>\n<tr>\n<td><strong>Tags<\/strong><\/td>\n<td>No Tags<\/td>\n<\/tr>\n<tr>\n<td><strong>Promoter<\/strong><\/td>\n<td>CMV5-CuO<\/td>\n<\/tr>\n<tr>\n<td><strong>Selection Marker<\/strong><\/td>\n<td>Bacterial: Kanamycin. Mammalian: Puromycin<\/td>\n<\/tr>\n<tr>\n<td><strong>Format<\/strong><\/td>\n<td>Lentiviral Plasmid<\/td>\n<\/tr>\n<tr>\n<td><strong>Viral Packaging<\/strong><\/td>\n<td><strong>abm<\/strong>\u00a0recommends using Cat#\u00a0<a href=\"https:\/\/www.abmgood.com\/Lentivirus-Transfection-Protocol.html\">G074, Lentifectin\u2122<\/a>\u00a0in conjunction with one of the following packaging mixes. The suggested packaging mix for this vector is Cat#\u00a0<a href=\"https:\/\/www.abmgood.com\/2nd-Generation-Lentivirus-Packaging.html\">LV003, 2nd Generation Packaging Mix<\/a>. Alternatively, you can also use Cat#\u00a0<a href=\"https:\/\/www.abmgood.com\/3rd-Generation-Lentivirus-Packaging.html\">LV053, 3rd Generation Packaging Mix<\/a><\/td>\n<\/tr>\n<tr>\n<td><strong>Appearance<\/strong><\/td>\n<td>Clear Liquid<\/td>\n<\/tr>\n<tr>\n<td><strong>Storage Buffer<\/strong><\/td>\n<td>10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n<tr>\n<td><strong>Stability<\/strong><\/td>\n<td>1 year when stored at -20\u00b0C or lower in a non-frost free freezer.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>NLRP1 Lentiviral Vector (Human) (Cumate) | \u8ca8\u865fiCu2055228 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":15124,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[88],"tags":[3031],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15123"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15123"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15123\/revisions"}],"predecessor-version":[{"id":15126,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15123\/revisions\/15126"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/15124"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15123"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15123"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15123"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}