{"id":15033,"date":"2017-11-03T14:15:45","date_gmt":"2017-11-03T06:15:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=15033"},"modified":"2022-07-21T09:52:20","modified_gmt":"2022-07-21T01:52:20","slug":"2x-qpcr-mastermix-rox-real-time-pcr-mastermix-%e8%b2%a8%e8%99%9fmastermix-r","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=15033","title":{"rendered":"2X qPCR MasterMix-ROX | Real time PCR Mastermix \u8ca8\u865fMasterMix-R"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=15033\"><strong>BrightGreen 2X qPCR MasterMix-ROX<\/strong><strong>\u8ca8\u865f<\/strong>MasterMix-R<\/a><\/span><\/p>\n<p>4 x 1.25 ml, for 500 reactions (20 \u00b5l)<br \/>\n<strong>\u7522\u54c1\u4ecb\u7d39<\/strong><strong>:<\/strong> \u4f7f\u7528abm\u7684BrightGreen 2X qPCR MasterMix\u4fdd\u8b49\u9ad8\u6027\u80fd\u5be6\u6642PCR\u3002 abm\u7684BrightGreen 2X qPCR MasterMix\u4ee5\u9810\u6df7\u5408\u548c\u512a\u5316\u7684\u5f62\u5f0f\u63d0\u4f9b\u5b9a\u91cfPCR\u5fc5\u9700\u7684\u6240\u6709\u6210\u5206\u3002 abm\u7684BrightGreen 2X qPCR MasterMix\u5177\u6709ROX\u6216\u87a2\u5149\u7d20\u4f5c\u70ba\u5167\u90e8\u88ab\u52d5\u53c3\u6bd4\u67d3\u6599\u7684\u9078\u9805\uff0c\u5728\u9748\u654f\u5ea6\uff0c\u4fe1\u566a\u6bd4\u548c\u5f15\u7269\u4e8c\u805a\u9ad4\u5b8c\u5168\u6d88\u9664\u65b9\u9762\u63d0\u4f9b\u4e86\u7121\u8207\u502b\u6bd4\u7684\u6027\u80fd\u3002<strong>\u4e3b\u8981\u7279<\/strong><strong>\u9ede:\u00a0<\/strong>\u6297\u9ad4hot-start\u71b1\u555f\u52d5\u6280\u8853\u63d0\u4f9b\u5353\u8d8a\u7684\u7279\u7570\u6027\uff0c\u6e96\u78ba\u91cf\u5316\u5404\u7a2e\u76ee\u6a19\u57fa\u56e0\uff0c\u6e1b\u5c11pipetting steps\u79fb\u6db2\u6b65\u9a5f\uff0c\u76e1\u91cf\u6e1b\u5c11\u6c61\u67d3\u98a8\u96aa\uff0c\u9069\u7528\u65bc\u5927\u591a\u6578real-time PCR\u5100\u5668\u00a0<strong>\u7522\u54c1\u61c9\u7528:\u00a0<\/strong>\u57fa\u56e0\u8868\u9054\u5206\u6790\uff0c\u57fa\u56e0\u6572\u9664\u9a57\u8b49\uff0c\u5fae\u9663\u5217\u9a57\u8b49\uff0c\u00a0High throughput applications\uff0c\u75c5\u6bd2\u6aa2\u6e2c\u548c\u91cf\u5316<strong>\u3002<\/strong><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/PCR\/images\/BrightGreen%20qPCR%20MasterMix-01.png\" alt=\"\" width=\"840\" height=\"237\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/PCR\/images\/BrightGreen_Thermal_Cycling_pic-01.png\" alt=\"\" width=\"816\" height=\"1155\" \/><\/p>\n<p>1.\u5206\u88dd\u8a66\u5291\u4ee5\u907f\u514d\u6c61\u67d3\uff0c\u4e26\u907f\u514d\u53cd\u8907\u7684\u51cd\u878d\u5faa\u74b0\u3002<br \/>\n2.\u5f15\u7269\u6fc3\u5ea6\u4e0d\u5b9c\u904e\u9ad8\uff0c\u6bcf\u7a2e\u5f15\u7269\u6fc3\u5ea6\u70ba100 nM\u81f3300 nM\u901a\u5e38\u6548\u679c\u6700\u597d\u3002<br \/>\n3.\u4e00\u500b\u975e\u5e38\u6709\u6548\u7684\u65b9\u6cd5\u4f86\u7372\u5f97\u91cd\u8907\u4e4b\u9593\u7dca\u5bc6\u7684Ct\u662f\u70ba\u4e86\u6e1b\u5c11\u79fb\u6db2\u8aa4\u5dee\uff0c\u9019\u53ef\u4ee5\u901a\u904e\u4ee5\u4e0b\u65b9\u6cd5\u4f86\u5be6\u73fe\uff1a\u88fd\u5099\u7279\u5b9a\u65bc\u64f4\u589e\u5b50\u7684\u9810\u6df7\u5408\u7269\uff0c\u4f7f\u7528\u91cd\u8907\u79fb\u6db2\u7ba1\uff0c\u4e26\u4e14\u5c07\u79fb\u6db2\u91cf\u4fdd\u6301\u5728\u88fd\u9020\u5efa\u8b70\u7bc4\u570d\u5167\u3002<br \/>\n4.\u70ba\u7372\u5f97\u6700\u4f73\u7d50\u679c\uff0c\u5efa\u8b70\u5f15\u7269\u9577\u5ea6\u70ba18-22\u500b\u6838\u82f7\u9178\uff0cTm\u70ba58\u2103-60\u2103\uff0c\u76ee\u6a19\u5927\u5c0f\u70ba100-250bp\u3002<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>BrightGreen 2X qPCR MasterMix-ROX\u8ca8\u865fMasterMix-R 4 x 1.25 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":2205,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[132],"tags":[3052],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15033"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15033"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15033\/revisions"}],"predecessor-version":[{"id":15037,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/15033\/revisions\/15037"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/2205"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15033"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=15033"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=15033"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}