{"id":14936,"date":"2017-10-23T12:00:13","date_gmt":"2017-10-23T04:00:13","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14936"},"modified":"2022-07-21T10:13:47","modified_gmt":"2022-07-21T02:13:47","slug":"sphingomyelinase-%e9%9e%98%e7%a3%b7%e8%84%82%e9%85%b6%e6%b4%bb%e6%80%a7%e8%9e%a2%e5%85%89%e6%b8%ac%e5%ae%9a-%e8%b2%a8%e8%99%9fk574-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=14936","title":{"rendered":"\u9798\u78f7\u8102\u9176\u6d3b\u6027\u87a2\u5149\u6e2c\u5b9a |  sphingomyelinase activity assay \u8ca8\u865fK574-100"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=14936\"><strong>\u9798\u78f7\u8102\u9176\u6d3b\u6027\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2 <\/strong><strong>| <\/strong><strong>\u8ca8\u865f<\/strong>K574-100<\/a><\/span><\/p>\n<p><strong><em>Simple assay to measure sphingomyelinase activity<\/em><\/strong><\/p>\n<p><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a<\/strong>\u9798\u78f7\u8102\u9176\uff08SMase\uff09\u5207\u5272\u9798\u78f7\u8102\u7522\u751f\u78f7\u9178\u81bd\u9e7c\u548c\u795e\u7d93\u91af\u80fa\u3002 SMase\u7684\u6d3b\u5316\u5c0e\u81f4\u795e\u7d93\u91af\u80fa\u7684\u7522\u751f\u589e\u52a0\uff0c\u5176\u4f5c\u70ba\u8102\u8cea\u7b2c\u4e8c\u4fe1\u4f7f\uff0c\u5176\u8a98\u5c0e\u5404\u7a2e\u7d30\u80de\u8abf\u7bc0\u73fe\u8c61\uff0c\u4f8b\u5982\u7d30\u80de\u51cb\u4ea1\uff0c\u7d30\u80de\u5206\u5316\uff0c\u7d30\u80de\u589e\u6b96\u548csterol homeostasis\u9ad4\u5167\u5e73\u8861\u3002 BioVision\u7684SMase\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u4f7f\u7528\u87a2\u5149\uff08Ex \/ Em = 535\/587 nm\uff09\u6e2c\u91cf\u4e2d\u6027SMase\u9176\u6d3b\u6027\u7684\u7c21\u55ae\u9748\u654f\u7684\u65b9\u6cd5\u3002 \u4e2d\u6027\u9798\u78f7\u8102\u9176\uff08N-SMase\uff09\u662f\u4e00\u7a2eMg2 +\u654f\u611f\u9176\uff0c\u53ef\u4ee5\u88ab\u4e00\u7cfb\u5217\u751f\u7406\u76f8\u95dc\u548c\u7d50\u69cb\u4e0d\u540c\u7684\u5206\u5b50\u6fc0\u6d3b\u3002 \u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cSMase\u5c07\u9798\u78f7\u8102\u5e95\u7269\u8f49\u5316\u70ba\u78f7\u9178\u81bd\u9e7c\u548c\u795e\u7d93\u91af\u80fa\uff0c\u7136\u5f8c\u5c07\u5176\u9032\u4e00\u6b65\u7528\u65bc\u7522\u751f\u87a2\u5149\u3002 \u9019\u7a2e\u9ad8\u901a\u91cf\u9069\u61c9\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u53ef\u4ee5\u5728\u5404\u7a2e\u6a23\u54c1\u4e2d\u6aa2\u6e2c\u4f4e\u81f310\u03bcU\/ ml\u7684\u9798\u78f7\u8102\u9176\u6d3b\u6027\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/5\/k574.jpg\" alt=\"\" \/><\/p>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Cat # +Size<\/strong><\/td>\n<td>K574-100<\/td>\n<\/tr>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>100 assays<\/td>\n<\/tr>\n<tr>\n<td><strong>Detection Method<\/strong><\/td>\n<td>Fluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>Measurement of SMase activity in various tissues\/cells extracts<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>\u2022 Simple, rapid &amp; convenient<br \/>\n\u2022 The assay can detect sphingomyleinase activity as low as 10 \u00b5U\/ml in a variety of samples.<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 SMase Assay Buffer<br \/>\n\u2022 SMase Probe (DMSO)<br \/>\n\u2022 SMase Substrate (Lyophilized)<br \/>\n\u2022 SMase Enzyme Mix I (Lyophilized)<br \/>\n\u2022 SMase Enzyme Mix II (Lyophilized)<br \/>\n\u2022 Choline Standard (Lyophilized)<br \/>\n\u2022 SMase Positive Control (Lyophilized)<br \/>\n\u2022 SMase Extraction Detergent<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u9798\u78f7\u8102\u9176\u6d3b\u6027\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK574-100 Simple assay to measure sphi [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[3073],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14936"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14936"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14936\/revisions"}],"predecessor-version":[{"id":14939,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14936\/revisions\/14939"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14936"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14936"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14936"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}