{"id":14781,"date":"2017-10-19T11:06:07","date_gmt":"2017-10-19T03:06:07","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14781"},"modified":"2022-07-21T10:16:12","modified_gmt":"2022-07-21T02:16:12","slug":"%e6%8a%97%e8%89%b1%e9%9b%a3%e6%a2%ad%e8%8f%8c%e8%b0%b7%e6%b0%a8%e9%85%b8%e8%84%ab%e6%b0%ab%e9%85%b6%ef%bc%88gdh%ef%bc%89%e5%a4%9a%e6%a0%aa%e6%8a%97%e9%ab%94-%e7%94%9f%e7%89%a9%e7%b4%a0%e6%a8%99","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=14781","title":{"rendered":"\u56f0\u96e3\u68ad\u72c0\u687f\u83cc\u8c37\u6c28\u9178\u812b\u6c2b\u9176\uff08GDH\uff09\u591a\u682a\u6297\u9ad4-\u751f\u7269\u7d20\u6a19\u8a18 |  Anti-Clostridium difficile Glutamate Dehydrogenase\u8ca8\u865f ACdGDH-B"},"content":{"rendered":"<p><span style=\"color: #00ccff;\">\u6297\u56f0\u96e3\u68ad\u72c0\u687f\u83cc<a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=14781\">\u8c37\u6c28\u9178\u812b\u6c2b\u9176\uff08GDH\uff09\u591a\u682a\u6297\u9ad4-\u751f\u7269\u7d20\u6a19\u8a18 <strong>| <\/strong><strong>\u8ca8\u865f<\/strong>\u00a0ACdGDH-B<\/a><\/span><\/p>\n<p>\u7522\u54c1\u4ecb\u7d39: \u56f0\u96e3\u68ad\u83cc\u8c37\u6c28\u9178\u812b\u6c2b\u9176\uff08GDH\uff09\u591a\u682a\u6297\u9ad4-\u751f\u7269\u7d20\u6a19\u8a18, \u5176\u514d\u75ab\u539f\u81ea\u56f0\u96e3\u68ad\u83cc\u8c37\u6c28\u9178\u812b\u6c2b\u9176\u9032\u884c\u96de\u96bb\u514d\u75ab\u3002\u5728\u5f17\u6c0f\u4f50\u5291\u4e2d\u9032\u884c\u591a\u6b21\u514d\u75ab\u5f8c\uff0c\u6536\u96c6\u86cb\uff0c\u4e26\u5c07IgY\u7d14\u5316\u3002 \u4f7f\u7528\u56fa\u5b9a\u5728\u56fa\u9ad4\u652f\u6301\u7269\u4e0a\u7684\u514d\u75ab\u539f\u5c07\u56f0\u96e3\u68ad\u83cc\u8c37\u6c28\u9178\u812b\u6c2b\u9176\u9032\u884c\u514d\u75ab\u89aa\u548c\u7d14\u5316\u3002<\/p>\n<p><strong>\u4f7f\u7528\u96de\u6297\u9ad4\u7684\u597d\u8655\uff1a<\/strong><\/p>\n<ul>\n<li>\u96de\u975e\u54fa\u4e73\u985e\u52d5\u7269\uff0c\u548c\u54fa\u4e73\u985e\u6297\u539f\u6709\u9ad8\u89aa\u548c\u6027\uff0c\u4e14\u907f\u514d\u548c\u54fa\u4e73\u985e\u6297\u539f\u7522\u751fcross-reaction\u3002<\/li>\n<li>\u70ba\u6700\u4eba\u9053\u7684\u591a\u682a\u6297\u9ad4\u7522\u88fd\u65b9\u6cd5\u3002\u53ea\u9700\u5f9e\u96de\u86cb\u8403\u53d6\u86cb\u767d\uff0c\u53d6\u6a23\u65b9\u4fbf\uff0c\u4e0d\u50b7\u5bb3\u52d5\u7269\u3002<\/li>\n<li>\u6bcf\u96bb\u96de\u53ef\u4ee5\u7522\u751f\u7684\u6297\u9ad4\u91cf\u7d043 g IgY\uff0f\u6708\uff0c\u662f\u5154\u5b50\u768410-20\u500d\u591a\u3002\u4e14\u7522\u88fd\u901f\u5ea6\u6bd4\u8d77\u5154\u5b50\u5feb\u4e0a\u8a31\u591a\uff0c\u6700\u5feb25\u5929\u5373\u53ef\u53d6\u5f97\u9ad8\u529b\u50f9\uff08titer\uff09\u6297\u9ad4\u3002<\/li>\n<li>\u96deIgY\u7684Fc region\u548c\u54fa\u4e73\u985eIgG\u7684\u4e0d\u540c\uff1a\n<ul>\n<li>\u53ef\u964d\u4f4e\u5c0d\u54fa\u4e73\u52d5\u7269\u7684\u80cc\u666f\u503c\u3002<\/li>\n<li>\u4e0d\u6703\u6d3b\u5316\u54fa\u4e73\u985e\u88dc\u88dc\u9ad4\u7cfb\u7d71\u3002<\/li>\n<li>\u4e0d\u6703\u8207\u54fa\u4e73\u985eFc recentors\u7d50\u5408\u3002<\/li>\n<li>\u7121\u6cd5\u548c\u5e38\u898f\u7684<a title=\"\u3010\u6297\u9ad4\u7d14\u5316\u3011Protein A\uff0fG\uff0fL\u86cb\u767d\u8cea\uff0f\u6297\u9ad4\u7d14\u5316\u76f8\u95dc\u7522\u54c1\uff0dBiovision\" href=\"http:\/\/biopioneer.com.tw\/?p=2332\">protein A<em>\uff08Staphylococcus aureus\uff09<\/em>\u3001protein G<em>\uff08Streptococcus sp.\uff09<\/em><em>\u3001<\/em>\u6216protein L<em>\uff08Peptostreptococcus magnus\uff09<\/em><\/a>\u7b49\u514d\u75ab\u7403\u86cb\u767d\uff0d\u86cb\u767d\u7d50\u5408\u53cd\u61c9\u3002<\/li>\n<li><img decoding=\"async\" src=\"http:\/\/gallusimmunotech.com\/images\/IgY.png\" alt=\"IgY\" \/><\/li>\n<\/ul>\n<\/li>\n<li>\u7531\u65bcIgY\u53d6\u5f97\u65b9\u4fbf\uff0c\u96de\u86cb\u53ef\u4ee5\u5132\u5b58\u8f03\u9577\u7684\u6642\u9593\uff0c\u4e26\u53ef\u7d14\u5316\u53d6\u5f97\u8f03\u4f73\u7684titer\u3002<\/li>\n<li>\u76f8\u5c0d\u65bc\u5154\u5b50\uff0c\u96de\u7684\u958b\u92b7\u8f03\u4fbf\u5b9c\u3002<\/li>\n<li>IgY\u6709\u4ee5\u4e0b\u6578\u7a2e\u548c\u54fa\u4e73\u985eIgG\u985e\u4f3c\u7684\u7279\u5fb5\uff1a\n<ul>\n<li>\u70ba\u4e8c\u50f9\u9ad4\u3002<\/li>\n<li>\u53ef\u7531papain\u5206\u89e3\u6210divalent Fab fragment\u3002<\/li>\n<li>\u9069\u7528<a title=\"\u3010\u9175\u7d20\u6297\u9ad4\u6a19\u5e5f\u3011Lightning-Link Enzyme\u2014Innova Bioscience\" href=\"http:\/\/biopioneer.com.tw\/?p=896\">\u9175\u7d20<\/a>\u3001<a title=\"\u3010\u6297\u9ad4\u6a19\u5e5f\u3011Lightning-Link Biotin \/ Streptavidin\u2014Innova Bioscience\" href=\"http:\/\/biopioneer.com.tw\/?p=902\">\u751f\u7269\u7d20\uff08biotin\uff09<\/a>\u3001<a title=\"\u3010\u5948\u7c73\u91d1\u3011InnovaCoat GOLD\u5948\u7c73\u91d1\u6a19\u5e5f\u5957\u7d44\uff0dInnova Bioscience\" href=\"http:\/\/biopioneer.com.tw\/?p=2603\">\u91d1\u7c92\u5b50<\/a>\u7b49\u6a19\u5b9a\u7269\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<table width=\"827\">\n<tbody>\n<tr>\n<td width=\"150\"><strong>Full Name<\/strong><\/td>\n<td>Biotin Conjugated Affinity Purified Chicken anti-Clostridium difficile Glutamate Dehydrogenase (GDH)<\/td>\n<\/tr>\n<tr>\n<td width=\"150\"><strong>Host<\/strong><\/td>\n<td>Chicken<\/td>\n<\/tr>\n<tr>\n<td width=\"150\"><strong>Quantity<\/strong><\/td>\n<td>0.1 mg<\/td>\n<\/tr>\n<tr>\n<td width=\"150\"><strong>Volume<\/strong><\/td>\n<td>0.1 mL<\/td>\n<\/tr>\n<tr>\n<td width=\"150\"><strong>Type<\/strong><\/td>\n<td>Biotin conjugated<\/td>\n<\/tr>\n<tr>\n<td width=\"150\"><strong>Physical State<\/strong><\/td>\n<td>Liquid<\/td>\n<\/tr>\n<tr>\n<td width=\"150\"><strong>Concentration<\/strong><\/td>\n<td>1.0 mg\/mL<\/td>\n<\/tr>\n<tr>\n<td width=\"150\"><strong>Diluent<\/strong><\/td>\n<td>Phosphate buffered saline\u201a pH 7.2\u201a 50% glycerol<\/td>\n<\/tr>\n<tr>\n<td width=\"150\"><strong>Preservative<\/strong><\/td>\n<td>0.01% Thimerosal<\/td>\n<\/tr>\n<tr>\n<td width=\"150\"><strong>Procedure<\/strong><\/td>\n<td>Chickens were immunized with highly purified Glutamate Dehydrogenase (GDH) purified from Clostridium difficile. After multiple immunizations in Freund&#8217;s adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile GDH was immunoaffinity purified using the immunogen immobilized on a solid support and conjugated to Biotin.<\/td>\n<\/tr>\n<tr>\n<td width=\"150\"><strong>Specifications<\/strong><\/td>\n<td>Optimal working dilutions should be determined for your particular assay conditions.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong style=\"color: #00ccff;\"><strong><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2017\/09\/tag6-e1505358740644-300x106.jpg\" alt=\"\u76f8\u95dc\u8a66\u5291\" width=\"95\" height=\"36\" \/>\u00a0\u00a0<\/strong><\/strong><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7832\">\u96deIgY\u6297\u9ad4\u7d14\u5316\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=8046\">Anti-Chicken\u591a\u682a\u6297\u9ad4-HRP<\/a>\u00a0|\u00a0\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=14791\">\u6297\u9f20\u7627\u539f\u87f2\u75c5\u6bd2-68 ORF59<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=14755\">\u70ad\u75bd\u82bd\u5b62\u687f\u83cc\u81f4\u6b7b\u56e0\u5b50A-FITC<\/a>\u00a0| anti-<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=14758\">\u70ad\u75bd\u82bd\u5b62\u687f\u83cc\u81f4\u6b7b\u56e0\u5b50A<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=14766\">\u6297\u72ac\u5fc3\u81df\u87f2\u00a0<\/a>|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=14771\">\u6297\u8089\u6bd2\u687f\u83cc\u795e\u7d93\u6bd2\u7d20E\u578b\u8f15\u93c8<\/a>\u00a0|\u00a0\u6297\u56f0\u96e3\u68ad\u72c0\u687f\u83cc<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=14781\">\u8c37\u6c28\u9178\u812b\u6c2b\u9176\uff08GDH\uff09<\/a><\/span><\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"113\">\u8ca8\u865f<\/td>\n<td width=\"329\">IgY\u6297\u9ad4\u5546\u54c1<\/td>\n<td width=\"152\">(Affinity purified)<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">DAIgYFab<\/td>\n<td width=\"329\">Donkey <a href=\"http:\/\/gallusimmunotech.com\/anti-chicken-igy-fab\/anti-chicken-igy-fab-donkey\"><strong>Anti-Chicken IgY Fab<\/strong><\/a><\/td>\n<td width=\"152\">0.5 mg<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">DAIgYFab-B<\/td>\n<td width=\"329\">\n<p>Donkey <a href=\"http:\/\/gallusimmunotech.com\/yolk-igy-purification-kit\/eggspress-igy-purification-kit-20-yolks\"><strong>Biotinylated Anti-Chicken IgY Fab<\/strong><\/a><\/p>\n<p>&nbsp;<\/td>\n<td width=\"152\">0.5 mg (BIOTIN)<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">DAIgYFab-F<\/td>\n<td width=\"329\">Donkey \u00a0<a href=\"http:\/\/gallusimmunotech.com\/anti-chicken-igy-fab\/fitc-labeled-anti-chicken-igy-fab-donkey\"><strong>FITC-labeled Anti-Chicken IgY Fab<\/strong><\/a><\/td>\n<td width=\"152\">0.5 mg (FITC)<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">DAIgYFab-HRP<\/td>\n<td width=\"329\">Donkey \u00a0<a href=\"http:\/\/gallusimmunotech.com\/anti-chicken-igy-fab\/hrp-labeled-anti-chicken-igy-fab\"><strong>HRP-labeled Anti-Chicken IgY Fab<\/strong><\/a><\/td>\n<td width=\"152\">0.5 mg (HRP)<\/td>\n<\/tr>\n<tr>\n<td width=\"113\">AIgYFab<\/td>\n<td width=\"329\">Goat <a href=\"http:\/\/gallusimmunotech.com\/anti-chicken-igy-fab\/anti-chicken-igy-fab-goat\"><strong>Anti-Chicken IgY Fab<\/strong><\/a><\/td>\n<td width=\"152\">0.5 mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a href=\"http:\/\/gallusimmunotech.com\/normal-chicken-igy\">Normal Chicken IgY<\/a> | <a href=\"http:\/\/gallusimmunotech.com\/normal-duck-igy\">Normal Duck IgY<\/a>| <a href=\"http:\/\/gallusimmunotech.com\/normal-goose-igy\">Normal Goose IgY<\/a>| <a href=\"http:\/\/gallusimmunotech.com\/anti-igy\">Anti-IgY<\/a> | <a href=\"http:\/\/gallusimmunotech.com\/anti-igy-immunoprecipitation-ip-reagents\">Anti IgY Immunoprecipitation (IP) Reagents<\/a> | <a href=\"http:\/\/gallusimmunotech.com\/other-anti-chicken-immunoglobulins\">Other Anti-Chicken Immunoglobulins<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6297\u56f0\u96e3\u68ad\u72c0\u687f\u83cc\u8c37\u6c28\u9178\u812b\u6c2b\u9176\uff08GDH\uff09\u591a\u682a\u6297\u9ad4-\u751f\u7269\u7d20\u6a19\u8a18 | \u8ca8\u865f\u00a0ACdGDH-B \u7522\u54c1\u4ecb\u7d39: \u56f0\u96e3\u68ad\u83cc\u8c37\u6c28 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":14756,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[3093],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14781"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14781"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14781\/revisions"}],"predecessor-version":[{"id":14802,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14781\/revisions\/14802"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/14756"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14781"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14781"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14781"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}