{"id":14283,"date":"2017-09-27T11:32:33","date_gmt":"2017-09-27T03:32:33","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14283"},"modified":"2022-07-21T10:32:20","modified_gmt":"2022-07-21T02:32:20","slug":"%e8%9b%8b%e7%99%bd%e9%85%aa%e6%b0%a8%e9%85%b8%e7%a3%b7%e9%85%b8%e9%85%b6%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a-protein-tyrosine-phosphatase-activity-assay-kit-fluorometric%e8%b2%a8%e8%99%9fk829","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=14283","title":{"rendered":"\u86cb\u767d\u916a\u6c28\u9178\u78f7\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a | Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric)\u8ca8\u865fK829"},"content":{"rendered":"<p><span style=\"color: #00ccff;\">\u00a0<img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.biovision.com\/skin\/frontend\/base\/default\/biovision\/logo\/biovision_logo.png\" alt=\"BioVision Logo\" width=\"184\" height=\"55\" \/><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=14283\" target=\"_blank\"><strong style=\"color: #00ccff;\">\u86cb\u767d\u916a\u6c28\u9178\u78f7\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\uff09<\/strong> <strong style=\"color: #00ccff;\">| <\/strong><strong style=\"color: #00ccff;\">\u8ca8\u865f<\/strong><span style=\"color: #00ccff;\">K829<\/span><\/a><\/span><\/p>\n<p>Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric)<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/8\/k829.jpg\" alt=\"\" width=\"167\" height=\"257\" \/><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/8\/k829-b.jpg\" alt=\"\" width=\"215\" height=\"198\" \/><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/8\/k829-c.jpg\" alt=\"\" width=\"184\" height=\"224\" \/><\/p>\n<p><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a<\/strong>\u86cb\u767d\u8cea\u916a\u6c28\u9178\u78f7\u9178\u9176\uff08PTPase\uff0cEC 3.1.3.48\uff09\u662f\u50ac\u5316\u5f9e\u86cb\u767d\u8cea\u78f7\u9178\u916a\u6c28\u9178\u6b98\u57fa\u53bb\u9664\u78f7\u9178\u916f\u90e8\u5206\u7684\u591a\u7a2e\u9176\u3002\u916a\u6c28\u9178\u6b98\u57fa\u7684\u78f7\u9178\u5316\u548c\u53bb\u78f7\u9178\u5316\u662f\u4e00\u7a2e\u5e38\u898b\u7684\u7ffb\u8b6f\u5f8c\u4fee\u98fe\uff0c\u7528\u4f5c\u8a31\u591a\u9176\uff0c\u53d7\u9ad4\u548c\u4fe1\u865f\u8f49\u5c0e\u9014\u5f91\u7684\u8abf\u7bc0\u958b\u95dc\u3002\u57fa\u65bc\u5b83\u5011\u7684\u7d30\u80de\u5b9a\u4f4d\uff0cPTP\u53ef\u4ee5\u5206\u985e\u70ba\u7d30\u80de\u5167\uff08\u975e\u53d7\u9ad4\uff09\u6216\u53d7\u9ad4\u6a23\u54c1\uff0c\u7136\u800c\u6240\u6709\u7d93\u5178PTPs\u90fd\u5171\u4eab\u4f7f\u7528\u89aa\u6838\u534a\u80f1\u6c28\u9178\u6b98\u57fa\u7684\u5e38\u898b\u50ac\u5316\u6a5f\u5236\u3002\u4f5c\u70ba\u7d30\u80de\u4fe1\u865f\u7d1a\u806f\u7684\u95dc\u9375\u8abf\u63a7\u56e0\u5b50\uff0cPTPs\u5f71\u97ff\u7d30\u80de\u751f\u9577\uff0c\u5206\u5316\uff0c\u9077\u79fb\u548c\u4ee3\u8b1d\u3002\u67d0\u4e9bPTP\u7684\u529f\u80fd\u969c\u7919\u8207\u5e7e\u7a2e\u4eba\u985e\u75be\u75c5\u6709\u95dc\u3002\u4f8b\u5982\uff0c\u7d30\u80de\u5167\u916a\u6c28\u9178\u78f7\u9178\u9176PTP1B\u662f\u80f0\u5cf6\u7d20\u4fe1\u865f\u50b3\u5c0e\u7d1a\u806f\u7684\u8ca0\u8abf\u7bc0\u56e0\u5b50\uff0c\u4e26\u4e14\u4e5f\u88ab\u8b49\u660e\u5728\u4eba\u4e73\u817a\u764c\uff0c\u7d50\u8178\u764c\u548c\u5375\u5de2\u764c\u4e2d\u986f\u8457\u904e\u8868\u9054\u3002 BioVision\u7684PTPase\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u53ef\u4ee5\u5feb\u901f\u6e2c\u91cfPTPase\u7684\u6d3b\u6027\uff0c\u5229\u7528\u8f49\u67d3\u6210\u9ad8\u87a2\u5149\u7522\u7269\uff08Ex \/ Em = 368\/460 nm\uff09\u7684\u87a2\u5149\u86cb\u767d\u78f7\u9178\u9176\u5e95\u7269\u3002\u63d0\u4f9b\u5ee3\u8b5cPTPase\u6291\u88fd\u5291\u7528\u65bc\u9a57\u8b49\u8907\u96dc\u751f\u7269\u57fa\u8cea\u4e2d\u7684\u6bd4\u6d3b\u6027\uff0c\u5176\u4e2d\u7d72\u6c28\u9178\/\u8607\u6c28\u9178\u78f7\u9178\u9176\u53ef\u80fd\u6709\u52a9\u65bc\u5e95\u7269\u4ee3\u8b1d\u3002\u8207ELISAs\u6216\u57fa\u65bc\u5b54\u96c0\u77f3\u7da0\u8272\u7684\u65b9\u6cd5\u4e0d\u540c\uff0c\u6e2c\u5b9a\u6cd5\u662f\u5747\u52fb\u7684\uff0c\u9023\u7e8c\u7684\uff0c\u4e26\u4e14\u4e0d\u9700\u8981\u5fa9\u96dc\u7684\u6a23\u54c1\u8655\u7406\u6216\u812b\u9e7d\u4ee5\u6d88\u9664\u6e38\u96e2\u78f7\u9178\u9e7d\u3002\u8a72\u6e2c\u5b9a\u64cd\u4f5c\u7c21\u55ae\uff0c\u9ad8\u901a\u91cf\u9069\u61c9\u6027\uff0c\u53ef\u6aa2\u6e2c\u81f3\u5c110.1 mU PTPase\u6d3b\u6027\u3002<\/p>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Cat # +Size<\/strong><\/td>\n<td>K829-100<\/td>\n<\/tr>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>100 assays<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Summary<\/strong><\/td>\n<td>\u2022 Detection method- Fluorescence (368\/440)<br \/>\n\u2022 Species reactivity- Mammalian<br \/>\n\u2022 Applications- The assay is designed to measure PTPase activity in biological samples with detection sensitivity ~100 \u00b5U<\/td>\n<\/tr>\n<tr>\n<td><strong>Detection Method<\/strong><\/td>\n<td>Fluorescence (368\/460 nm)<\/td>\n<\/tr>\n<tr>\n<td><strong>Species Reactivity<\/strong><\/td>\n<td>Mammalian<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>Rapid assessment of PTPase activity in biological samples or recombinant PTPase preparations<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>\u2022 Simple procedure; takes less than 1 hour<br \/>\n\u2022 Fast and convenient<br \/>\n\u2022 The kit is more sensitive than colorimetric assays using phosphate detection.<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 PTPase Assay Buffer<br \/>\n\u2022 Disulfide Reducing Agent (DTT)<br \/>\n\u2022 Fluorescent Standard<br \/>\n\u2022 PTPase Inhibitor (Suramin)<br \/>\n\u2022 PTPase Substrate<br \/>\n\u2022 PTPase Positive Control<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u00a0\u86cb\u767d\u916a\u6c28\u9178\u78f7\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\uff09 | \u8ca8\u865fK829 Protein Tyrosine Phosphat [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[468],"tags":[3168],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14283"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14283"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14283\/revisions"}],"predecessor-version":[{"id":14287,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14283\/revisions\/14287"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14283"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14283"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14283"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}